Score: 4.18
#242 - SMAEuNetbiol - A network-biology based approach for the development of SMN-independent treatments
We propose a network-biology based approach to detect and test SMN-independent treatment options. The approval of Spinraza has been a milestone for the treatment of SMA the last years. However, still there are a number of non-responders and many patients have been symptomatic for years. This is a significant problem since a delayed intervention with Spinraza reduces its therapeutic benefit. Since SMN has already been enhanced in Spinraza-treated patients other complementary approaches have to be taken into account. Those are termed SMN-independent approaches. Previously, we and others reported a number of changed signaling pathways with differing potentials as SMN-independent treatments targets. However, signaling pathways act in a network and this network character has been neglected in the SMA-field so far. Thus, we present novel data on a systems-biology approach towards altered signaling in SMA. We used presymptomatic and onset Taiwanese SMA-mice to generate a network of pathways allowing an informed decision for highly connected targets. Those are predicted to redress the equilibrium of the whole network. We will employ a C. elegans SMA model to screen through the network by multiple rescue and phenocopying experiments allowing us to identify the most potent targets. A validation of these targets on the functional level in C. elegans and an identification of the affected cell-type within the spinal cord of Taiwanese SMA-mice, will allow us to design specific AAV-based treatment approaches. A targeted treatment of the affected cell type in the spinal cord will reduce off-target effects in other cells or tissues. This is important to prevent side-effects a pre-requisite for a now milder clinical phenotype. We will test one target identified by this pipeline in a pre-clinical trial combined with Spinraza. However, our network-biology based pipeline already allowed us to identify B-Raf as a possible target for an SMN-independent approach. B-Raf is an important neurotrophic signaling hub, which is pre-symptomatically down-regulated in spinal cord neurons. Thus we will intrathecally co-administer an AAV2/1-syn-B-Raf0 x Flag together with the ASO-10-27 (the pharmacological active substance in Spinraza) to Taiwanese SMA-mice.
Kind: project - Date: Sep 01, 2020
Score: 3.93
#1871 - In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag
Visone V, Han W, Perugino G, del Monaco G, She Q, Rossi M, Valenti A, Ciaramella M
Kind: pub - Date: Nov 30, 2017
Score: 3.56
#1921 - Unconventional Transport Routes of Soluble and Membrane Proteins and Their Role in Developmental Biology
Pompa A, De Marchis F, Pallotta MT, Benitez-Alfonso Y, Jones A, Schipper K, Moreau K, Zárský V, Di Sansebastiano GP, Bellucci M
Kind: pub - Date: Nov 30, 2017
Score: 3.51
#1991 - Misidentified human gene functions with mouse models: the case of the retinoblastoma gene family in senescence
Alessio N, Capasso S, Ferone A, Di Bernardo G, Cipollaro M, Casale F, Peluso G, Giordano A, Galderisi U
Kind: pub - Date: Jan 18, 2018
Score: 3.24
#1410 - Structural Biology of Bacterial Haemophores
Ascenzi P, di Masi A, Leboffe L, Frangipani E, Nardini M, Verde C, Visca P
Kind: pub - Date: Apr 08, 2016
Score: 3.22
#2841 - Recent developments in the synthesis of oligosaccharides by hyperthermophilic glycosidases
Moracci M, Cobucci-Ponzano B, Perugino G, Giordano A, Trincone A, Rossi M
Kind: pub - Date: May 26, 2020
Score: 2.79
#2272 - The DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) of Caenorhabditis elegans is involved in meiosis and early development under physiological conditions
Serpe M, Forenza C, Adamo A, Russo N, Perugino G, Ciaramella M, Valenti A
Kind: pub - Date: Jul 07, 2019
Score: 2.48
#3282 - Nanoalgosomes: Introducing extracellular vesicles produced by microalgae
Adamo G, Fierli D, Romancino DP, Picciotto S, Barone ME, Aranyos A, Božič D, Morsbach S, Raccosta S, Stanly C, Paganini C, Gai M, Cusimano A, Martorana V, Noto R, Carrotta R, Librizzi F, Randazzo L, Parkes R, Capasso Palmiero U, Rao E, Paterna A, Santonicola P, Iglič A, Corcuera L, Kisslinger A, Schiavi ED, Liguori GL, Landfester K, Kralj-Iglič V, Arosio P, Pocsfalvi G, Touzet N, Manno M, Bongiovanni A
Kind: pub - Date: Sep 06, 2021
Score: 2.42
#170 - Nutri-C@rgo - Characterization of plant secreted nanovesicles
Introduction and study aims Discovered 30 years ago, mammalian extracellular vesicles (EVs), including exosomes, microsomes and apoptotic bodies have shown to be implicated in a novel form of cell-cell communication; and thus represent an important and rapidly growing research field in biology and translational sciences. More recently, plants were also shown to secrete membrane-bound particles. Today, two classes of EVs have been isolated from plants: apoplastic vesicles from rice, sunflower and tomato; and exosome-like particles from fruit juices such as grapes and grapefruits. It is well established that a plant-derived diet has great influence on regulation of homeostasis of the cells lining the digestive system. Deregulation of intestinal epithelial cell homeostasis, for example, mediates chronic inflammation (like inflammatory bowel diseases), increased propensity to develop infections and cancer. In a recent study, Mu et al reported that edible plant derived exosome-like nanoparticles can be taken up by intestinal macrophages and stem cells where they induce anti-inflammatory processes. Interspecies communication between plant and mammalian cells through nanoparticles is a new and very interesting field which opens up new avenues for further studies. In contrast to the mammalian EVs there is a limited information available about the bio-cargo of plant derives nanoparticles. Here we propose to study the molecular cargo of EVs isolated from fruit juices of the citrus family. The molecular data will be used to answer the following questions: i.) what is the molecular cargo of citrus derived EVs ii) what are the differences among EVs isolated from different citrus species iii.) how much does the protein and lipid cargo of plant EVs differ from that of mammalian cells and, iv.) what are the possible interlocutors involved in the crosstalk between plant EVs and mammalian cells The obtained data sets will be deposited into two manually curated web-based databases, EVpedia and Vesiclepedia, that are publicly available and at the moment lack such data. Motivation for co-operation The two research groups, Mass Spectrometry and Proteomics Laboratory at IBBR-OUS-Napoli and Core Technologies Centre of the Hungarian Academy of Sciences (CTC) are working in complementary fields, and the principal investigators (PIs) have a long-standing collaboration. PIs already led joint projects in the past and have published together. Recently, they have been collaborating in the characterization of mammalian EVs using mass spectrometry and chromatography-based techniques. They both have relevant expertise in the food sector as well.PIs recently launched collaborations with the research group at IBBR-OUS Perugia, Italy and the research group of Cell- and Immunobiology of the Department of Genetics, Semmelweis University, Budapest, Hungary on the exploitation of extracellular vesicles. The expertise of the principal and partner investigators and their collaborators in the exosome field is well-documented by an existing patent application, scientific papers and book chapters published in the last five years. The relevant expertise and the available instrumentation put the CNR-MTA team, the proponent of this project, in an excellent position to establish joint research collaboration through NutriC@rgo. The ultimate aim of the project is to set up a collaborative network in order to prepare successful applications of European collaborative research projects under Horizon2020 collaborative Research and Innovation projects and research networks (COST actions)
Kind: project - Date: Sep 23, 2016
Score: 2.41
#286 - Non disponibile - Identificazione di estratti naturali con azione neuroprotettiva per lo sviluppo di nutraceutici.
Il progetto è mirato all’identificazione e caratterizzazione di molecole naturali, con azione neuroprotettiva. Il progetto si focalizza su tematiche di interesse per l’industria nutraceutica, per la produzione di alimenti funzionali con capacità di neuroprotezione e di prevenzione di malattie neurodegenerative. Anche l’industria farmaceutica può trarne giovamento, per la produzione di farmaci innovativi per le malattie neurodegenerative. Le malattie neurodegenerative di origine genetica, pur essendo rare, causano gravi costi socio-economici e sono caratterizzate da una cattiva prognosi. Inoltre l’Italia, per l’elevata longevità della sua popolazione, ha la più alta proporzione al mondo di ultrasessantacinquenni (oltre il 22%). L’allungamento della vita è associato ad un aumento delle spese per la salute, dato che la prevalenza di malattie aumenta con l’età e, dopo gli ottant’anni, circa il 50% della popolazione ha due o più patologie croniche, di cui le più frequenti sono quelle neurodegenerative. Per questi motivi l’identificazione di nuove molecole neuroprotettive è di grande interesse per il mercato e rientra nelle tematiche previste dalla Strategia Nazionale di Specializzazione Intelligente (SNSI), approvata dalla Commissione Europea, ed in particolare nell’area tematica nazionale Salute, alimentazione, qualità della vita, nonchè nelle tematiche Horizon 2020 previste dal Work Programme 2018-2020 (Health, demographic change and wellbeing) e Horizon Europe 2021 (Goal n.3 good health and well-being), nell’ambito degli obiettivi di crescita sostenibile delle Nazioni Unite. La presente proposta prevede lo svolgimento dell’attività di ricerca in 24 mesi nel laboratorio del dott. Di Schiavi presso l’IBBR (Napoli), istituto CNR convenzionato con l’Università della Campania; 6 mesi nell’azienda Arterra Bioscience S.p.A. (Napoli); e 6 mesi presso l’Institute of Neuroanatomy and Cell Biology del Hannover Medical School (Germania). Nello specifico, il laboratorio del dott. Di Schiavi (IBBR) si impegna nel fare apprendere al dottorando le tecniche per lo studio in vivo degli effetti di estratti, miscele e molecole naturali sul modello C.elegans. Arterra Bioscience si impegna per formarlo sui temi della validazione delle biomolecole identificate presso l’IBBR, mediante biosaggi di attività su cellule umane in coltura. L’Institute of Neuroanatomy and Cell Biology si impegna perchè apprenda le più recenti tecniche utilizzando modelli cellulari di roditori di malattie neurodegenerative per la validazione delle biomolecole identificate presso l’IBBR. Il percorso formativo proposto assicurerà al dottorando di acquisire competenze in una tematica di grande interesse in ambito di Innovazione & Ricerca, come testimoniato dal crescente interesse di molte aziende che operano nel settore biomedico, nutraceutico, cosmeceutico e della Green-Pharmacy.
Kind: project - Date: Nov 01, 2021
Score: 2.29
#897 - Périgord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis
Francis M, Kohler A, Murat C, Balestrini R, Coutinho PM, Jaillon O, Montanini B, Morin E, Noel, Percudani R, Porcel B, Rubini A, Amicucci A, Amselem J, Anthouard V, Arcioni S, Artiguenave F, Aury JM, Ballario P, Bolchi A, et al
Kind: pub - Date: Feb 09, 2015
Score: 2.15
#3497 - Editorial: A Systems View of Plant Cellular Communication
Di Silvestre D, Tadini L, Trotta A, Valledor L, Salekdeh GH, Jorrin Novo JV
Kind: pub - Date: Sep 09, 2022
Score: 1.91
#1368 - Myeloma cells can corrupt senescent mesenchymal stromal cells and impair their anti-tumor activity
Ozcan S, Alessio N, Acar MB, Toprak G, Gonen ZB, Peluso G, Galderisi U
Kind: pub - Date: Feb 10, 2016
Score: 1.89
#59 - Resolving the black box of PFAS toxicty: Using a whole-body organism to explore and define toxic endpoints
In vitro cell-based assays lack the ability to quantitatively link whole-body adverse outcomes with human-relevant molecular events that have been perturbed by chemicals at a cellular level. To “resolve the black box” it is more informative to integrate a whole organism 3R approach with an in vitro toxicity testing approach. The nematode Caenorhabditis elegans is ideal for this, not least because it has high sequence similarity to humans with no ethical constraints. In my talk, I will introduce the advantages of working with C. elegans and demonstrate its applicability to toxicological testing using a group of man-made chemicals, Per- and polyfluorinated alkylated substances (PFAS). Despite PFAS manufacture being banned in the EU, elevated levels of the chemicals are still detected in the environment and in the population, the health relevant effects of PFAS exposure in unclear and the specific mechanism(s) of PFAS toxicity has yet to be fully elucidated. We have therefore initiated a multi-disciplinary research line to explore how to firmly place C. elegans in the regulatory toxicity testing pipeline, making use of advances in molecular biology, computational modelling and analytical chemistry
Kind: webinars - Date: Oct 26, 2023
Score: 1.86
#223 - XXXXX - Journey to the cold and back: comparative genomics and transcriptomics in Antarctic and sub-Antarctic notothenioids
Notothenioids represent a large group of phylogenetically related fish that evolved in the progressively cooling Antarctic waters and show remarkable biochemical and physiological modifications compared to temperate teleosts. Oxygen transport is one of the major physiological processes that underwent extraordinary changes in notothenioids, reaching the extreme of complete loss of hemoglobin in the “white-blooded” icefish. While notothenioids are mostly endemic to the Southern Ocean, a few species have secondarily migrated to sub-Antarctic waters, returning to environmental conditions similar to those experienced by marine fish living in a temperate environment as their ancestors. The main objective of the project is to produce an unprecedented set of whole-transcriptome sequence data across notothenioid species as well different physiological/ecological conditions. Results will be used in a comparative approach to investigate the role of gene expression and protein-coding gene evolution in the adaptation of notothenioids to the Antarctic marine environment. To this end, the project combines genomics with comparative transcriptomics targeting key notothenioid species, which are most diverse in terms of evolutionary history (temperate/sub-Antarctic outgroups), geographic distribution (high Antarctic vs secondarily sub-Antarctic), and physiological/ecological features (red-blooded vs hemoglobinless). The project will gather a highly multidisciplinary team of scientists who will integrate cutting-edge genomics, bioinformatics, and statistics with in-depth knowledge of fish physiology, mitochondrial biology, and Antarctic biology toward a comprehensive analysis on the evolution of Antarctic fish and their response to changing environmental conditions. Collaborations with several international research groups will be established or continued to ensure optimal sampling and data interpretation. Strong links also exist with other project proposals in the current call, allowing further integration and synergy.
Kind: project - Date: Nov 14, 2017
Score: 1.86
#2065 - Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe
Rossi F, Morrone C, Massarotti A, Ferraris DM, Valenti A, Perugino G, Miggiano R
Kind: pub - Date: Jun 20, 2018
Score: 1.85
#1126 - The Arabidopsis AtRGGA RNA binding protein regulates tolerance to salt and drought stress.
Ambrosone A, Batelli G, Nurcato R, Aurilia V, Punzo P, Bangarusamy DK, Ruberti I, Sassi M, Leone A, Costa A, Grillo S
Kind: pub - Date: Mar 25, 2015
Score: 1.49
#2056 - The launch of a European network of research infrastructure facilities for Industrial Biotechnology (IBISBA 1.0)
Launch of a Horizon2020-Infraia starting community project to support industrial biotechnology. A pan-european community of infrastructure to support industrial biotechnology. On the 01/12/2017, the Ibisba 1.0 project was launched. This project entitled Industrial Biotechnology Innovation and Synthetic Biology Accelerator 1.0, aims to create a coordinated network of research infrastructure facilities that support industrial biotechnology
Kind: news - Date: May 25, 2018
Score: 1.43
#227 - XXXXXX - Neurodegeneration versus Neuroprotection: Role of estrogen-induced neuroglobin expression
The role of globins in the brain seems to be pivotal to support nervous system functions during temporary period of hypoxia, which may follow environmental challenges and pathologic insults, therefore they avoid serious damages in the nervous system. Only in 2000, the first vertebrate nerve globin, named neuroglobin (Ngb), has been identified in neuronal tissues of mice and humans. Since its identification, several evidences highlighted a protective role of Ngb over-expression against neurodegeneration by preventing neuronal apoptosis and mitochondrial damage. Thus, the increase of Ngb levels could have a therapeutic benefit in brain disorders. Because mammalian Ngb is an intracellular protein and does not cross cell membranes, the administration of Ngb is not a feasible therapeutic strategy. Recent results indicate that the sex steroid hormone 17beta-estradiol (E2) is an endogenous modulator of Ngb, increasing Ngb levels of about 300% in different neuronal populations. This conspicuous effect is at the root of the well known E2 protective effects on the brain. The eight research Units proposing this project represent a joint research group synergistically focused to discover agents influencing the expression levels of Ngb and its role in altered neuronal cells in order to obtain new drugs modulating its expression which may be useful in the treatment and prevention of neurological diseases. Therefore, the main goals of this three-years-long project are: i) the characterization of mechanisms underlying Ngb overexpression in the brain; ii) the clarification of Ngb functions in the brain; iii) the elucidation of Ngb involvement in models of neurodegeneration; and iv) the design and production of new molecules inducing Ngb overexpression. Beyond the interest from a fundamental biology viewpoint, this study will uncover novel proteins able to bind to Ngb and estrogen receptors or pathways in which Ngb and estrogen receptors are involved. These new pathways can be potential targets for the development of drugs for the prevention and treatment of specific neurological diseases including Huntington’s disease and glaucoma. From a basic science point of view, the present research program is expected to produce a large amount of data on the role played by Ngb on neurodegenerative diseases and on the role played by estrogen in the brain. Another of the main aims of this grant proposal is the rational design of molecules which can modulate estrogen receptor signalling and Ngb levels. This will be done either by modifying the pre-existing natural compounds known to bind estrogen receptors and to modulate their activities or by synthesizing novel compounds (non-peptidic and peptidic). A critical mass of Italian scientists with diverse expertise (ranging from chemistry, biochemistry, pathology, and neurology) and diverse experimental approaches (molecular, cellular, bioinformatics, and clinical approaches) is involved in the present project. Furthermore, the partners of the present proposal have already established contacts and collaborations with seven European laboratories generating a Consortium that will achieve added value by sharing skills, different perspectives, expertise, and resources, guaranteeing a constructive discussion and collaboration between different ways to approach problems and find solutions. This critical mass also contributes to the trans-disciplinarily approach required by such an ambitious project which is availing itself of recent cutting-hedge knowledge-based and technological advances ranging from proteomics to phage-display and bioinformatics techniques. We believe that this assortment of people will ensure the experience necessary to achieve our goals.
Kind: project - Date: Feb 01, 2013
Score: 1.37
#244 - Giorgia Batelli(personal page)
Role: Researcher Section: Researchers and Technologists Position Researcher at IBBR/CNR Work experience 2003-2007: PhD Student at the University of Naples, Federico II 2005-2006: Exchange student at the University of California, Riverside laboratories of Prof. Jian-Kang Zhu 2008 - 2009: Post-doctoral fellow at the University of Turin, laboratory of Prof. Andrea Schubert and Claudio Lovisolo 2009-2011: Post-doctoral fellow at the King Abdullah University of Science and Technology (KAUST) and University of California, Riverside (UCR), laboratories of Prof. Jian-Kang Zhu 2011- : Researcher at IGV (renamed IBBR from 2013), Research Division of Portici, CNR. Education: Batchelor Degree: University of Naples, Federico II, 2003 PhD: University of Naples, Federico II, 2007 Research Interests The stress group at CNR-IBBR in Portici is interested in understanding the molecular and genetic aspects of plant abiotic stress responses, with a focus on post-transcriptional and post-translational mechanisms leading to stress tolerance in Arabidopsis thaliana and Solanaceous species. Identifying genetic and epigenetic regulation of reproductive pathways is the focus of research aimed at creating new tools for plant breeding. Technologies including forward and reverse genetics, RNA silencing, omics combined with single-cell are used in the current projects dealing with the following topics: Isolation and functional analysis of genes involved in drought and salt stress responses in arabidopsis and tomato Ecotype-specific drought stress responses in tolerant/sensitive genotypes of tomato
Kind: personal - Date: Jul 25, 2014
Score: 1.34
#1986 - Every OGT is illuminated… by fluorescent and synchrotron lights
Miggiano R, Valenti A, Rossi F, Rizzi M, Perugino G, Ciaramella M
Kind: pub - Date: Jan 18, 2018
Score: .97
#34 - IBBR webinars / New insights in bone biology from exome sequencing of rare skeletal diseases
IBBR webinars (Jan 13, 2021) Eleonora Palagano, IBBR/UOS Sesto Fiorentino - Whole exome sequencing (WES) is a powerful tool to identify new molecules involved in skeletal homeostasis. In particular we used WES to establish the molecular diagnosis of two particular skeletal diseases: osteopetrosis and the acrofrontofacionasal dysostosis 1 (AFFND1). The osteopetroses are a group of rare bone diseases characterized by increased bone density due to the failure in bone resorption. Due to their genetic heterogeneity, WES represents a valuable strategy to identify the genetic defect and to help in the differential diagnosis. Regarding AFFND1, this is an extremely rare syndrome, comprising facial and skeletal abnormalities, short stature and intellectual disability. WES found a novel truncating mutation in the neuroblastoma-amplified sequence (NBAS) gene in two patients. This mutation impaired NBAS functions in HEK293T cells overexpressing the truncated NBAS protein. Furthermore, we demonstrated that NBAS expression in mouse embryos was compatible with a role in bone and brain development and that the depletion of endogenous z-nbas in fish embryos resulted in defective morphogenesis of chondrogenic cranial skeletal elements. Overall, we provided evidence supporting the hypothesis of a causative role of the mutated NBAS gene in the pathogenesis of AFFND1. In conclusion, we effectively exploited WES in the genetic diagnosis of rare skeletal diseases. We also highlighted potential limitations of this approach, specifically with respect to deep intronic mutations and synonymous changes, and underlined the importance to complement WES with analysis at the transcript level and functional validation.
Kind: video - Date: Jan 13, 2021
Score: .95
#318 - Simone Grisan(personal page)
Born in Udine, on 4th september 1979, since 2006 engaged in research activity in the areas of plant pathology, biodiversity of endophytic community, plant/pathogen interaction, adopting microbiological and molecular methodology including innovative technology...
Kind: personal - Date: Sep 16, 2014
Score: .89
#410 - Fulvio Pupilli(personal page)
Actual Research sectors Analysis of plant reproductive system through molecular approaches. Actually Dr.Pupilli is studying apomixis, that is a clonal plant reproduction by seeds. The overall objective of the research is the isolation of the genetic determinants of apomixis in a wild apomictic species Paspalum simplex with the perspective to develop an apomixis system for crops. Main achievements are: i) characterization and mapping of the apomixis locus, ii) establishing synteny relationships with rice genome, iii) isolation of candidate genes through partial sequencing of apomixis-linked BAC and comparative transcriptomic analysis. Recent Scientific Activities. A) Induction of new variability by somatic hybridization B) Molecular markers of the analysis of natural and inducted genetic A) During the first phase of his career Dr. Pupilli obtained and characterized several somatic hybrid plants combining the genome of alfalfa with those of M.coerulea (S+C), M.arborea (S+A) and M.falcata (S+F). B) After a stage at the MPI of Cologne, Dr. Pupilli used molecular markers to study the genome composition of somatic hybrids and the genetic variability in forage and turf plant populations. Page in preparation)
Kind: personal - Date: Oct 21, 2014
Score: .88
#3 - New insights in bone biology from exome sequencing of rare skeletal diseases
Whole exome sequencing (WES) is a powerful tool to identify new molecules involved in skeletal homeostasis. In particular we used WES to establish the molecular diagnosis of two particular skeletal diseases: osteopetrosis and the acrofrontofacionasal dysostosis 1 (AFFND1). The osteopetroses are a group of rare bone diseases characterized by increased bone density due to the failure in bone resorption. Due to their genetic heterogeneity, WES represents a valuable strategy to identify the genetic defect and to help in the differential diagnosis. Regarding AFFND1, this is an extremely rare syndrome, comprising facial and skeletal abnormalities, short stature and intellectual disability. WES found a novel truncating mutation in the neuroblastoma-amplified sequence (NBAS) gene in two patients. This mutation impaired NBAS functions in HEK293T cells overexpressing the truncated NBAS protein. Furthermore, we demonstrated that NBAS expression in mouse embryos was compatible with a role in bone and brain development and that the depletion of endogenous z-nbas in fish embryos resulted in defective morphogenesis of chondrogenic cranial skeletal elements. Overall, we provided evidence supporting the hypothesis of a causative role of the mutated NBAS gene in the pathogenesis of AFFND1. In conclusion, we effectively exploited WES in the genetic diagnosis of rare skeletal diseases. We also highlighted potential limitations of this approach, specifically with respect to deep intronic mutations and synonymous changes, and underlined the importance to complement WES with analysis at the transcript level and functional validation
Kind: webinars - Date: Jan 13, 2021
Score: .82
#340 - Silvana Grandillo(personal page)
Main Scientific Interests Development of mapped genetic resources in tomato, with special emphasis given to populations of marker-defined introgression lines (ILs) originating from interspecific crosses, for a more efficient exploitation of the natural biodiversity stored in unadapted germplasm. Current applications of these genetic resources include QTL analyses for fruit quality traits and integrative systems biology and genomic approaches to gain further insights into the networks regulating key fruit metabolic pathways underlying tomato flavour and nutritional quality. Identification and functional characterization of key genes involved in nutritional and merceological quality in pepper. Metabolic engineering of secondary metabolite pathways for the production of health-related compounds.
Kind: personal - Date: Oct 03, 2014
Score: .65
#2584 - Vincenzo D’Amelia(personal page)
Main research topics Since I started my scientific career, I have been fascinated by plant metabolites and, in particular, the genetics behind their control. I am keenly interested in unlocking the molecular regulation of these extraordinary natural compounds to better understand their role in plant cells and also in exploiting them for human needs.
Kind: personal - Date: Apr 08, 2020
Score: .60
#378 - Roberto Defez(personal page)
Molecular plant-microbe crosstalk, nitrogen fixation, homone regulation, legumes, cereals
Kind: personal - Date: Oct 16, 2014
Score: .58
#394 - Francesca Bagnoli(personal page)
Positions Present: researcher at CNR IBBR Firenze, Italy since October 2014 Previous: researcher at CNR IPSP Firenze, Italy November 2011- September 2014 2002 - Ph.D. in Agricultural and Forest Genetics at the University of Florence, discussing a thesis:...
Kind: personal - Date: Oct 17, 2014
Score: .57
#1753 - "Alimentazione oggi: tra nutraceutica ed epigenetica" - Napoli, Città della Scienza, 03/05/2017
L’incontro è promosso dall’Accademia Nazionale delle Scienze detta dei XL in collaborazione con il CNR, Università di Napoli Federico II e IDIS Città della Scienza nell’’ambito del ciclo di incontri su ‘Scienza e tecnica nell’’Antropocene: rischi e benefici’, momenti di riflessione sullo sviluppo di un ‘buon antropocene’.
Kind: news - Date: Apr 27, 2017
Score: .47
#72 - Maria Stefania Grillo(personal page)
Current appointment: Head of the Portici Division of the IBBR/CNR. Research Interest My research group has long experience on the applications of biotechnologies to plant breeding of vegetables crops. Research activities are focused on the identification, characterization and transfer of genes involved in the plant response to environmental stresses. We are using functional genomics approaches in Solanacee and Arabidopsis through large scale transcriptome analyses in response to osmotic and temperature stress, and characterization of crucial gene functions by means of forward and reverse genetics approaches. I am also involved in the development of molecular and biotech tools for the use of plant as biofactories for the production of bio-molecules of industrial value.
Kind: personal - Date: Dec 09, 2013
Score: .47
#413 - Clemente Capasso(personal page)
Clemente Capasso is a researcher at the National Research Council, Italy. His research interest from 1992 to date has been the study of the structure/function relationship of both native and recombinant proteins from terrestrial, marine organisms and pathogenic microorganisms. This allowed him to acquire extensive knowledge of most protein purification procedures as well as kinetic, chemical-physical, and structural characterization of enzymes. He has written 13 book chapters and published over 150 papers. He acquired an intense training in Italian and international laboratories. He is the Associate Editor of Journal of Enzyme Inhibition and Medicinal Chemistry as well as a member of the Editorial Board of Expert Opinion on Therapeutic Patents, Current Enzyme Inhibition, and International Journal of Molecular Science.
Kind: personal - Date: Oct 22, 2014
Score: .25
#2672 - Alessandra Ruggiero(personal page)
Principali competenze: Manipolazione di DNA ed RNA: Tecniche del DNA ricombinante, clonaggi; purificazione di DNA ed RNA (da cellule e tessuti, animali e vegetali); PCR, RT-PCR, qPCR; Mutagenesi sito specifica, Sequenziamento ed analisi di sequenze di DNA ed RNA; Clonaggio molecolare da lieviti, roditori, batteri e piante; Trasformazione genetica stabile e transiente di specie vegetali; Southern e northern blotting; • Espressione, purificazione ed analisi di proteine: Sistemi di espressione proteica in lievito, batteri, piante (piante intere e colture cellulari), Yeast 2-hybrid assay, elettroforesi di proteine e Western blotting; • Approcci di Forward Genetics: Generazione di mutanti TDNA ed EMS; Screening di fenotipi mutanti; Isolamento di mutazioni attraverso TAIL PCR; Complementazione di mutanti; Trasformazione di pianta mediate da Agrobacterium; • Approcci di Reverse Genetics: Analisi In silico di espressione genica e proteica, knockdown mediante RNAi; • Colture in vitro: Esperienza in colture cellulari (animali e vegetali); trasfezione di linee cellulari; tecniche di immunoistochimica e immunocitochimica; Tecniche di lavoro e propagazione in ambiente sterile. • Colture in vivo: Allevamento di specie vegetali in pieno campo ed in serra, in condizioni controllo ed in condizioni di stress idrico e/o salino; • Tecniche di fisiologia vegetale: Misurazioni fisiologiche di stress ossidativo e deficit idrico; Misurazione parametri biochimici (valutazione ABA e prolina) su tessuti differenti in diverse specie vegetali. Esperienza nell’uso di strumenti per la fluorescenza e fotosintesi, porometri. • Studi in vivo con animali di piccola taglia: Gestione delle colonie di topi (accoppiamenti e svezzamenti anche di transgenici); Genotipizzazione, dissezione e analisi di tessuti. In ambito informatico: Ottima conoscenza dei sistemi operativi Microsoft Windows e MacOsX; del pacchetto completo Microsoft Office; dei programmi di visualizzazione grafica (Adobe PhotoShop). Ottima capacità di utilizzo di motori di ricerca (PubMed, Medline) della rete internet e di banche dati (PDB, sequenze genomiche). In ambito bioinformatico: Analisi avanzata di sequenze di DNA e di proteine mediante l’uso di pacchetti software disponibili in rete (Blast, Prosite, pFAM e altri) e nell’utilizzo di banche dati di interesse biologico. Buona conoscenza delle tecnologie genomiche avanzate e bioinformatiche applicate al miglioramento genetico di specie vegetali, in particolare per l’assemblaggio delle sequenze; mappaggio delle reads lungo un genoma di riferimento; annotazione di sequenze genomiche; consultazione ed utilizzo di sequenze genomiche annotate; analisi di dati RNA-Seq di campioni di RNA vegetale: assemblaggio ed annotazione de novo di trascrittomi e digital gene expression profiling utilizzando i principali software disponibili in rete.
Kind: personal - Date: May 01, 2020
