Francesca De Marchis
Role: Senior Researcher
Section: Researchers and Technologists
Division: Perugia
Tel: (39) 075-5014810
E-mail: francesca.demarchis@ibbr.cnr.it
Date of birth:17/01/1973
Place of work: Institute of Biosciences and Bioresources (IBBR), CNR, via Madonna Alta 130, 06128 Perugia, Italy
Telephone: 0755014810
E-mail: francesca.demarchis@ibbr.cnr.it
WORKING EXPERIENCE
- (2023-at present) Senior Researcher at IBBR - CNR, via Madonna Alta 130, 06128 Perugia, Italy
- (2012-2022) Researcher at IBBR - CNR, via Madonna Alta 130, 06128 Perugia, Italy
- (2000-2012) research fellow, term contracts at IBBR - CNR, via Madonna Alta 130, 06128 Perugia, Italy. Type of employment or sector: Genetics and Biology and plant biotechnology
- (1999-2000) Research fellowship at the Experimental Zooprophylactic Institute of Umbria and Marche . Type of employment or sector: production of a vaccine against classical swine fever in plants.
EDUCATION AND TRAINING
- (2011-2014) PhD in "Biology and Ecology", University of Perugia
- (1992-1997) Degree in Biological Sciences, University of Perugia
RESEARCH ACTIVITY
Genetic, biology and plant biotechnology
SCIENTIFIC ACTIVITIES OF INTEREST
- Chloroplast biotechnology
Genetic engineering of tobacco chloroplast in order to accumulate proteins of agronomic, pharmaceutical and industrial interest. Transformation of sugar beet and unicellular alga Chlamydomonas reinhardtii chloroplasts. Studies on the stability of recombinant proteins within the chloroplast and. Exploiting of plastids for the synthesis of vegetable biopolymers of protein origin. Study of alternative selection markers for chloroplast trasformation.
- Non-food crops
Production of proteins for industrial and pharmaceutical use in photosynthetic organisms. Search for vegetable molecules to be used as adjuvants for antibiotic therapy and anti-bacterial biofilm action.
- Protein secretion
Analysis of the different aspects of secretory proteins traffic. Study of the mechanisms that regulate the unconventional trafficking of proteins within the plant cell. Mechanisms that control the synthesis, folding and transport of proteins in the plant cell
Scopus H index: 17 WOS H index 16
Perugia April 2024
Selected Publications
(full list available at CNR People)
A biotechnological approach for the production of new protein bioplastics
De Marchis F, Vanzolini T, Maricchiolo E, Bellucci M, Menotta M, Di Mambro T, Aluigi A, Zattoni A, Roda B, Marassi V, Crinelli R, Pompa A
Year: 2024
ORIGIN OF RECOGNITION COMPLEX 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
Bellucci M, Caceres ME, Paolocci F, Vega JM, Ortiz JP, Ceccarelli M, De Marchis F, Pupilli F
Year: 2023
Genetic transformation of plant cells as a possible strategy for the production of bioactive compounds or biopolymers
Maricchiolo E, Bellucci M, De Marchis F, Fraternale D, Paolocci F, Zattoni A, Roda B, Marassi V, Pompa A
Year: 2022
Unconventional Pathways of Protein Secretion: Mammals vs. Plants
Maricchiolo E, Panfili E, Pompa A, De Marchis F, Bellucci M, Pallotta MT
Year: 2022
Human Indoleamine 2.3-dioxygenase 1 (IDO1) Expressed in Plant Cells Induces Kynurenine Production
Bellucci M, Pompa A, De Marcos Lousa C, Panfili E, Orecchini E, Maricchiolo E, Fraternale D, Orabona C, De Marchis F, Pallotta MT
Year: 2021
Plastid transformation in Sugar Beet: an important industrial crop
De Marchis F, Bellucci M
Year: 2021
Perspectives on protein biopolymers: miniaturized flow field-flow fractionation-assisted characterization of a single-cysteine mutated phaseolin expressed in transplastomic tobacco plants
Marassi V, De Marchis F, Roda B, Bellucci M, Capecchi A, Reschiglian P, Pompa A, Zattoni A
Year: 2021
Class IA PI3Ks regulate subcellular and functional dynamics of IDO1
Iacono A, Pompa A, De Marchis F, Panfili E, Greco FA, Coletti A, Orabona C, Volpi C, Belladonna ML, Mondanelli G, Albini E, Vacca C, Gargaro M, Fallarino F, Bianchi R, De Marcos Lousa C, Mazza EM, Bicciato S, Proietti E, Milano F, Martelli MP, Iamandii IM, Graupera Garcia-Amila M, Llena Sopena J, Hawkins P, Suire S, Okkenhaug K, Stark A, Grassi F, Bellucci M, Puccetti P, Santambrogio L, Macchiarulo A, Grohmann U, Pallotta MT
Year: 2020
New antimicrobial devices to prevent waterborne diseases
Palmieri G, Cammarano A, Agrillo B, Proroga YT, Gogliettino M, Balestrieri M, De Stefano L, Battisti M, De Martino S, Mercurio ME, Cummaro A, Nicolais L
Year: 2020
Transfer of a mutant plant glutamate 1-semialdehyde aminotransferase gene from the nuclear to the plastid genome confers gabaculine resistance in tobacco
Bellucci M, De Marchis F, Pompa A, Micheli M, Gardi T, Rosellini D
Year: 2019
Synthesis, purification and analysis of new plant biopolymers
De Marchis F, Bellucci M, Aluigi A, Zattoni A, Roda B, Marassi V, Morosinotto T, Pompa A
Year: 2019
Unconventional protein secretion in plant cells: three cases study
De Marchis F, Bellucci M, Pallotta MT, Iacono A, Grohmann U, Pompa A
Year: 2019
Host-endosymbiont co-evolution shaped chloroplast translational regulation
De Marchis F, Pompa A, Ballottari M, Bellucci M
Year: 2019
A single cystein-enriched phaseolin expressed in transplastomic tobacco plants accumulates as a biopolymer
Capecchi A, De Marchis F, Bellucci M, Fallarino F, Zattoni A, Marassi V, Roda B, Pompa A
Year: 2018
Expression of CLAVATA3 fusions indicates rapid intracellular processing and a role of ERAD
De Marchis F, Colanero S, Klein EM, Mainieri D, Prota VM, Bellucci M, Pagliuca G, Zironi E, Gazzotti T, Vitale A, Pompa A
Year: 2018
Erratum to “Expression of CLAVATA3 fusions indicates rapid intracellular processing and a role of ERAD” [Plant Sci. 271 (2018) 67-80]
De Marchis F, Colanero S, Klein EM, Mainieri D, Prota VM, Bellucci M, Pagliuca G, Zironi E, Gazzotti T, Vitale A, Pompa A
Year: 2018
Relationship between functions and intracellular localization of the immune checkpoint target indoleamine 2.3-dioxygenase 1
Iacono A, Pompa A, De Marchis F, Bellucci M, Grassi F, Grohmann U, Pallotta MT
Year: 2018
Characterization of a single cystein-enriched phaseolin expressed in transplastomic tobacco plants
Zattoni A, Capecchi A, De Marchis F, Bellucci M, Fallarino F, Marassi V, Roda B, Pompa A
Year: 2018
The endoplasmic reticulum is a hub to sort proteins toward unconventional traffic pathways and endosymbiotic organelles
Bellucci M, De Marchis F, Pompa A
Year: 2017
Expression of the plant GSA gene from the plastid genome
Bellucci M, De Marchis F, Pompa A, Micheli M, Veronesi F, Rosellini D
Year: 2017
Unconventional Transport Routes of Soluble and Membrane Proteins and Their Role in Developmental Biology
Pompa A, De Marchis F, Pallotta MT, Benitez-Alfonso Y, Jones A, Schipper K, Moreau K, Zárský V, Di Sansebastiano GP, Bellucci M
Year: 2017
Transient expression of clv3-gfp indicates rapid intracellular processing of clv3 and a role of endoplasmin in its synthesis
Pompa A, Klein EM, Mainieri D, Prota VM, De Marchis F, Vitale A
Year: 2017
Expression of human FVIII coagulation factor in plants
Scattini G, De Marchis F, Bellucci M, Matino D, Fallarino F, Pompa A
Year: 2017
Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation
De Marchis F, Bellucci M, Pompa A
Year: 2016
Chemical secretory pathway modulation in plant protoplasts
De Marchis F, Pompa A, Bellucci M
Year: 2016
Overexpression of the olive acyl carrier protein gene (OeACP1) produces alterations in fatty acid composition of tobacco leaves
De Marchis F, Valeri MC, Pompa A, Bouveret E, Alagna F, Grisan S, Stanzione V, Mariotti R, Cultrera N, Baldoni L, Bellucci M
Year: 2016
Olive fruits infested with olive fly larvae respond with an ethylene burst and the emission of specific volatiles
Alagna F, Kallenbach M, Pompa A, De Marchis F, Rao R, Baldwin IT, Bonaventure G, Baldoni L
Year: 2015
A mutant Synechococcus gene encoding glutamate 1-semialdehyde aminotransferase confers gabaculine resistance when expressed in tobacco plastids
Bellucci M, De Marchis F, Ferradini N, Pompa A, Veronesi F, Rosellini D
Year: 2015
Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation
De Marchis F, Bellucci M, Pompa A
Year: 2015
Increased oleic acid content in tobacco leaves due to the expression of an olive acyl carrier protein
De Marchis F, Valeri MC, Pompa A, Bouveret E, Alagna F, Grisan S, Stanzione V, Mariotti R, Cultrera N, Baldoni L, Bellucci M
Year: 2015
An autoregulatory mechanism modulates translation of heterologous proteins in tobacco chloroplast
Pompa A, Bellucci M, De Marchis F
Year: 2015
Isolation and molecular characterization of three acyl carrier protein genes in olive (Olea europaea L.)
Cultrera NGM, Alagna F, Mariotti R, De Marchis F, Pompa A, Bellucci M, Baldoni L
Year: 2014
Plastid Transformation in Sugar Beet: Beta vulgaris
De Marchis F, Bellucci M
Year: 2014
Obtainment of fertile and genetically stable transplastomic sugar beet plants over three generations
De Marchis F, Pompa A, Bellucci M
Year: 2014
Traffic of Human α-Mannosidase in Plant Cells Suggests the Presence of a New Endoplasmic Reticulum-to-Vacuole Pathway without Involving the Golgi Complex
De Marchis F, Bellucci M, Pompa A
Year: 2013
Unconventional pathways of secretory plant proteins from the endoplasmic reticulum to the vacuole bypassing the Golgi complex
De Marchis F, Bellucci M, Pompa A
Year: 2013
Overexpression of an olive acyl carrier protein (ACP) in tobacco tissuues.
De Marchis F, Pompa A, Cultrera N, Mariotti R, Alagna F, Baldoni L, Bellucci M
Year: 2013
Plastid proteostasis and heterologous protein accumulation in transplastomic plants
De Marchis F, Pompa A, Bellucci M
Year: 2012
Human a-mannosidase produced in transgenic tobacco plants is processed in human alpha-mannosidosis cell lines
De Marchis F, Balducci C, Pompa A, Hilde R, Stensland MF, Guaragno M, Pagiotti R, Menghini AR, Persichetti E, Beccari T, Bellucci M
Year: 2011
A plant secretory signal peptide targets plastome-encoded recombinant proteins to the thylakoid
De Marchis F, Pompa A, Mannucci R, Morosinotto T, Bellucci M
Year: 2011
Enzyme Replacement Therapy: Production of Human ?-Mannosidase in Transgenic Tobacco Plants
De Marchis Francesca, Balducci Chiara, Pagiotti Rita, Arcioni Sergio, Beccari Tommaso, Bellucci Michele
Year: 2010
An engineered C-terminal disulfide bond can partially replace the phaseolin vacuolar sorting signal
Pompa A, De Marchis F, Vitale A, Arcioni S, Bellucci M
Year: 2010
Endoplasmic reticulum signal peptide enhances folding of a chimeric protein when expressed in chloroplast
Pompa Andrea, De Marchis Francesca, Mannucci Roberta, Bellucci Michele
Year: 2010
Genetic transformation of the sugar beet plastome
De Marchis F, Wang Y, Stevanato P, Arcioni S, Bellucci M
Year: 2009
Agrobacterium mediated genetic transformation of alfalfa
Bellucci M, De Marchis F, Arcioni S
Year: 2008
The human immunodeficiency virus antigen Nef forms protein bodies in leaves of transgenic tobacco when fused to zeolin
De Virgilio M, De Marchis F, Bellucci M, Mainieri D, Rossi M, Benvenuto E, Arcioni S, Vitale A
Year: 2008
Protein domains involved in assembly in the endoplasmic reticulum promote vacuolar delivery when fused to secretory GFP, indicating a protein quality control pathway for degradation in the plant vacuole
Foresti O, De Marchis F, de Virgilio M, Klein EM, Arcioni S, Bellucci M, Vitale A
Year: 2008
Zeolin is a recombinant storage protein that can be used to produce value-added proteins in alfalfa (Medicago sativa L.)
Bellucci M, De Marchis F, Arcioni S
Year: 2007
Zeolin is a recombinant storage protein with different solubility and stability properties according to its localization in the endoplasmic reticulum or in the chloroplast
Bellucci M, De Marchis F, Nicoletti I, Arcioni S
Year: 2007
Cytoplasm and chloroplasts are not suitable subcellular locations for ß-zein accumulation in transgenic plants
Bellucci M, De Marchis F, Mannucci R, Bock R, Arcioni S
Year: 2005
Transformation of Lotus corniculatus plants with Escherichia coli asparagines synthetase A: effect on nitrogen assimilation and plant development
Bellucci M, Ederli L, De Marchis F, Pasqualini S, Arcioni S
Year: 2004
Zeolin, a new recombinant storage protein constructed using maize à‚Æ’×-zein and bean phaseolin
Mainieri D, Rossi M, Archinti M, Bellucci M, De Marchis F, Vavassori S, Pompa A, Arcioni S, Vitale A
Year: 2004
Jellyfish green fluorescent protein as a useful reporter in Medicago sativa L. for transient expression and stable transformation
Bellucci Michele, De Marchis Francesca, Mannucci Roberta, Arcioni Sergio
Year: 2003
Analisi della diffusione ambientale di polline transgenico di leguminose foraggere
De Marchis F, Bellucci M, Arcioni S
Year: 2003
Measuring gene flow from two birdsfoot trefoil (Lotus corniculatus) field trials using transgenes as tracer markers
De Marchis Francesca, Bellucci Michele, Arcioni Sergio
Year: 2003
