Characterization of EVs by proteomic and lipidomic analyses to investigate the roles of extracellular vesicles

Kwang Pyo Kim 
Department of Applied Chemistry, Kyung Hee University - Corea

April 28, 2023 (11:00-12:00)
Webinar Link: https://meet.goto.com/694540357

Abstract: In this presentation, we focus on the application of mass spectrometry to understand the molecular complexity of plant-derived extracellular vesicles (EVs) and present the analysis of EVs from ginseng as an example of this mass spectrometry application. While EVs derived from mammalian or bacterial cells are well characterized, the secretion, uptake, physical characteristics, and vesicular components of plant-derived EVs are poorly understood. The molecular complexity of plant-derived EVs necessitates high-throughput analytical platforms for detailed analysis. Recently, mass spectrometry and bioinformatics tools have enabled detailed proteomic, lipidomic, and metabolomic analyses of EVs. Our lipidomic analysis of ginseng-derived EVs revealed significant increases in diacylglycerols, phosphatidylcholine, phosphatidylethanolamine, lysophosphatidylcholine, and sphingomyelin, which are unique vesicular properties. Furthermore, we investigated the effects of ginseng-derived EVs on human dermal fibroblasts and ultraviolet B radiation-treated human melanocytes. Our results demonstrated that ginseng-derived EV treatments improved the replicative senescent or senescence-associated pigmented phenotypes by downregulating senescence-associated molecules and/or melanogenesis-related proteins. Our study provides new insights into the molecular complexity and biological effects of plant-derived EVs and may pave the way for future therapeutic applications as bioactive and sustainable nanomaterials for improving tissue conditions and targeted delivery of active constituents

Author's Info: http://fsb.khu.ac.kr/member/professor.htm