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Protein-controlled programmable nucleic acid nanodevices for sensing application

Alessandro Porchetta 
Università degli Studi di Roma - Tor Vergata - Italy

November 09, 2022 (11:30-12:30)
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Abstract: Integrating dynamic nucleic acid (NA)-based devices with protein-controlled actuation will expand our ability to process molecular information. In the past few years, we have developed different strategies to control simple NA-based systems using proteins and antibodies [1]. Most of them take advantage of the engineering of synthetic switches, aptamers and more complex reactions that convert specific protein-binding events into measurable outputs [2]. Recently, we have reported on synthetic switches capable of transducing repair enzyme activity into measurable FRET signal changes [3]. Motivated by the above results, we aim now at developing the potential of our molecular systems in performing further programmable complex tasks by combining functional nucleic acids with CRISPR-based systems. [1] Porchetta A et al. Programmable nucleic acid nanoswitches for the rapid, single-step detection of antibodies in bodily fluids. Journal of the American Chemical Society. 2018 Jan 24;140(3):947-53. [2] Rossetti M et al. Harnessing effective molarity to design an electrochemical dna-based platform for clinically relevant antibody detection. Angewandte Chemie. 2020 Aug 24;132(35):15083-8. [3] Farag N et al. Folding-upon-Repair DNA Nanoswitches for Monitoring the Activity of DNA Repair Enzymes. Angewandte Chemie. 2021 Mar 22;133(13):7359-65

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Istituto di Bioscienze e Biorisorse (IBBR/CNR)
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