National Research Council of Italy

Institute of Biosciences and BioResources

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IBBR publication #1091

Effects of cryopreservation on germinability of olive (olea europea L.) pollen

Alba V, Bisignano V, Alba E, De Stradis A, Polignano GB

Genetic resources and crop evolution 58 (7): 977-982. (2011)
doi: 10.1007/s10722-011-9736-z

Long term storage of viable pollen is important for bank germplasm constitution to preserve resources that can be used in breeding programs, biotechnologies and genetic engineering. Pollen from 12 olive (Olea europaea L.) cultivars was stored for 1 year in liquid nitrogen at -196°C. The morphology of pollen grains and germination rates on fresh and long term stored pollen were observed. Results on in vitro pollen germinability, both before and after cryopreservation, showed highly significant responses among the 12 cultivars. The relationship between germinability and pollen grain size did not reveal any significant relationships in both treatments. Our findings will contribute to the improvement of olive pollen preservation and lead to find a more efficient method for its long term storage, while retaining its germinability

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