Immacolata Fiume
Role: CTER-IV
Section: Technicians
Division: Naples
Tel: (39) 081-6132585-081-6132570
E-mail: immacolata.fiume@ibbr.cnr.it
URL: http://ibbr.cnr.it
EDUCATION AND TRAINING
1989 Diploma in Biological chemistry, “Istituto Tecnico Industriale Statale “Francesco Giordani”, Naples, Italy.
1992-1994 Study grant International Mass Spectrometry Centre of CNR, Naples, Italy.
WORKING EXPERIENCE
- from 1th of January 2014 Mass Spectrometry and Proteomic Laboratory-Institute of Biosciences and Bioresources, CNR - UOS of Naples, Italy
- 2002-2012 Mass Spectrometry and Proteomic Laboratory-Institute of Protein Biochemistry, CNR - Naples, Italy
- 1994-2002 Intitute of Food Science and Technology, CNR - Avellino, Italy
- 1990-1994 International Mass Spectrometry Centre, CNR - Naples, Italy
FIELD OF RESEARCH
- Protein Biochemistry;
- Protein purification and characterization;
- Mass Spectrometry and Proteomics;
- Phosphoproteomics;
- Glycoproteomics;
- ITRAQ and ICAT labeling for quantitative mass spectrometry analysis of proteins;
- Exploiting exosomes for biomarker discovery studies;
- Proteomics charecterizations of cell secretome and secretion mechanisms;
- Identification of new diagnostic/prognostic biomarkers, and drug targets for the development of new therapeutic approaches
- Development of new mass spectrometry-based analytical methods.
INVOLVEMENT IN RESEARCH PROJECTS
- “Quantitative proteomics for the identification of differentially expressed proteins in cells treated with Daunomycin bioconjugates” CNR-MTA Agreement CNR/MTA agreement -
- 2010/2012.
- “Identification of reliable urinary biomarkers of Diabetic Nephrology by means of powerful and complementary proteomics strategyies” Pogetto Società Italiana di Nefrologia (SIN) - Ricercando 2011.
- “Nuovi networks molecolari per il controllo dell’omeostasi energetica: implicazioni per il diabete di tipo 2 e l’obesità”, MIUR 2010-2013
TECHNICAL PROFICIENCY
- Isoelectrofocusing, different elecrophoresis techniques (1D & 2D PAGE), Western blotting, , Gaschromatography, High Performance Liquid Chromatography (RP, HILIC, HPAE), Mass Spectrometry (FAB-MS, GC-MS, MALDI-TOF, LC-MS/MS, SELDI-TOFMS), Enzymatic assays.
- Proteomic bioinformatics: Scaffold, Mascot.
Selected Publications
(full list available at CNR People)
Protein Biocargo and Anti-Inflammatory Effect of Tomato Fruit-Derived Nanovesicles Separated by Density Gradient Ultracentrifugation and Loaded with Curcumin
Mammadova R, Maggio S, Fiume I, Bokka R, Moubarak M, Gellén G, Schlosser G, Adamo G, Bongiovanni A, Trepiccione F, Guescini M, Pocsfalvi G
Year: 2023
Bilateral project CNR-CINVESTAV mEVChip: A microfluidic device to assess the anti-inflammatory effects of ginger-derived nanovesicles on mammalian cells
Pocsfalvi G, Fiume I, Ramos AP, J-L G-C, Amador Hernandez J, Rodriguez R
Year: 2021
Pursuing mechanisms of extracellular vesicle formation. Effects of sample processing
Božič D, Hočevar M, Kononenko V, Jeran M, Štibler U, Fiume I, Pajnič M, PaÄàen L, Kogej K, Drobne D, Iglič A, Pocsfalvi G, Kralj-Iglič V
Year: 2020
Plant roots release small extracellular vesicles with antifungal activity
De Palma M, Ambrosone A, Leone A, Del Gaudio P, Ruocco M, Turiák L, Bokka R, Fiume I, Tucci M, Pocsfalvi G
Year: 2020
Potential allergenicity of Medicago sativa investigated by a combined IgE-binding inhibition, proteomics and in silico approach
Yakhlef M, Giangrieco I, Ciardiello MA, Fiume I, Mari A, Souiki L, Pocsfalvi G
Year: 2020
Bacterial IAA-delivery into Medicago root nodules triggers a balanced stimulation of C and N metabolism leading to biomass increase
Defez R, Andreozzi A, Romano S, Pocsfalvi G, Fiume I, Esposito R, Angelini C, Bianco C
Year: 2019
Bacterial IAA-Delivery into Medicago Root Nodules Triggers a Balanced Stimulation of C and N Metabolism Leading to a Biomass Increase
Defez R, Andreozzi A, Romano S, Pocsfalvi G, Fiume I, Esposito R, Angelini C, Bianco C
Year: 2019
Dissection of protein cargo of Citrus fruit juice sac cells-derived vesicles reveals heterogeneous transport and extracellular vesicles subpopulations
Pocsfalvi G, Turiák L, Ambrosone A, Del Gaudio P, Puska G, Fiume I, Silvestre T, Vékey K
Year: 2018
Chromatography and its hyphenation to mass spectrometry for extracellular vesicle analysis
Pocsfalvi G, Stanly C, Fiume I, Vékey K
Year: 2016
Mass spectrometry of extracellular vesicles
Pocsfalvi G, Stanly C, Vilasi A, Fiume I, Capasso G, Turiák L, Buzas EI, Vékey K
Year: 2016
Isolation and characterisation of a novel alpha-amylase from the extreme haloarchaeon Haloterrigena turkmenica
Santorelli M, Maurelli L, Pocsfalvi G, Fiume I, Squillaci G, La Cara F, Del Monaco G, Morana A
Year: 2016
Isolation of exosome-like vesicles from plants by ultracentrifugation on sucrose/deuterium oxide (D2O) density cushions
Stanly C, Fiume I, Capasso G, Pocsfalvi G
Year: 2016
Urinary extracellular vesicles as reservoirs of altered proteins during the pathogenesis of polycystic kidney disease
Pocsfalvi G, Raj DA, Fiume I, Vilasi A, Trepiccione F, Capasso G
Year: 2015
Surface-exposed Glycoproteins of Hyperthermophilic Sulfolobus solfataricus P2 Show a Common N-Glycosylation Profile
Palmieri G, Balestrieri M, Peter-Katalinic J, Pohlentz G, Rossi M, Fiume I, Pocsfalvi G
Year: 2013
Enrichment specificity of micro and nano-sized titanium and zirconium dioxides particles in phosphopeptide mapping
Vilasi A, Fiume I, Pace P, Rossi M, Pocsfalvi G
Year: 2013
Urinary exosomes for protein biomarker research
Amal Raj DA, Fiume I, Capasso G, Pocsfalvi G
Year: 2012
Structural and Functional Insights into Aeropyrum pernix OppA, a Member of a Novel Archaeal OppA Subfamily
Balestrieri M, Gogliettino M, Fiume I, Pocsfalvi G, Catara G, Rossi M, Palmieri G
Year: 2011