National Research Council of Italy

Institute of Biosciences and BioResources

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Maddalena De Virgilio

Role: Researcher
Section: Researchers and Technologists
Division: Bari
Tel: (39) 0805583400-214


Permanent researcher at the Institute of Biosciences and Bioresources (former Institute of Plant Genetics), CNR, Bari, Italy.


December 2008: permanent position as researcher at Institute of Plant Genetics of the National Research Council of Bari, Italy.

September 2004: research fellow at at CNR-IBBA-Milano; project within Pharmaplanta consortium: “Recombinant Pharmaceuticals from Plants for Human Health”.

November 2000: research associate at Department of Cell Biology- Division of Vascular Biology- The Scripps Research Institute- La Jolla-California USA; on “Detection in living cells of the interactions involving integrins cytoplasmic domains by Biolouminescence Resonance Energy Transfer and Bimolecular Fluorescence Complementation”.

September 1999: research fellow at Dipartimento di Medicina Sperimentale e Diagnostica-Sezione di Patologia Generale-Università degli Studi di Ferrara; on “Study on the molecular mechanisms responsable for redox potential of living cells. Role of signal transduction in the control of cell proliferation”.

June 1999: PhD on ““ER associated degradation of a short-lived glycoprotein from the lumen of the endoplasmic reticulum: the role of ubiquitination and N-linked glycans” at Department of Molecular Genetics-University and Biocenter Vienna, Austria.

April 1994: EMBO fellowship at Uppsala Genetic Center- Department of Cell Research-Uppsala-Sweden on “Study on the expression of trypsin protease inhibitors in white mustard by in situ hybridization”.

March 1994: Professional qualification in Biology

November 1992: Graduated in Biology at the University of Bari


Citizen science


Biodiversity census at the urban coast.

Participation to H2020 MARINA Project (Marine Knowledge Sharing Platform for Federating Responsible Research and Innovation Communities). Coordinator: Dr. Fernando Ferri at Institute for Research on Population and Social Policies.


de Virgilio M., Cifarelli S., Garofoli G., Massari V, Nitti A, Degryse B. Toxic algal blooms at the urban coast: a citizens’ point of view. Act in Conference GECOMARS 2020-International workshop on ecosystem based management. 4-5 February 2020. Marseille-France. Vie et Milieu . In press

de Virgilio M., Cifarelli S., De Gennaro P, Garofoli G., Degryse B. A first attempt of citizen science in the genetic monitoring of a Posidonia oceanica meadow in the Italian Southern Adriatic Sea. Journal for Nature Conservation, 2020, 56: 125826

de Virgilio M., Degryse B. Harnessing the Destructive Power of Ricin to Fight Human Cancer. Chapter in book. Publisher: Bentham Science. 2014. DOI: 10.2174/9781608058785114010016

de Virgilio M., Silvestris F. Urokinase receptor (uPAR) ligand based recombinant toxins for human cancer therapy. Current Pharmaceutical Design, 2011 17. Doi : 10.2174/138161211796718170

de Virgilio M., Lombardi L., Calandro R., Frabbrini M.S. Ribosome-Inactivating Proteins: from Plant Defense to Tumor Attack. Tossine (Basilea), 2010 2: 2699-737. Doi: 10.3390 / tossine2112699.

Avesani L., Vitale A., Pedrazzini L., de Virgilio M., Pompa A., Barbante A., Gecchele E., Dominaci P., Morandini F., Brozzetti A., Falorni A., Pezzetti M. Recombinant human GAD65 accumulates to high levels in transgenic tobacco plants when expressed as an enzymatically inactive mutant. Plant Biotechnol J., 2010 8: 862-72. Doi: 10.1111/j.1467-7652.2010.00514.x.

de Virgilio, Francesca De Marchis, Michele Bellucci, Davide Mainieri,Marika Rossi, Eugenio Benvenuto, Sergio Arcioni and Alessandro Vitale. The Human Immunodeficiency Virus Antigen Nef Forms Protein Bodies in Leaves of Transgenic Tobacco When Fused to Zeolin. J. Exp. Bot. 2008 59: 2815-29. Doi: 10.1093 / jxb / ern143

de Virgilio M., Kiosses W.B., and Shattil S.J.Proximal, selective, and dynamic interactions between integrin {alpha}IIb{beta}3 and protein tyrosine kinases in living cells. J Cell Biol. 2004 165: 305-311. Doi: 10.1083 / jcb.200402064.

Foresti O., Frigerio L., Holkeri H., de Virgilio M, Vavassori S., and A Vitale. A phaseolin domain involved directly in trimer assembly is a determinant for binding by the chaperone BiP.Plant Cell. 2003 15: 2464-75. Doi: 10.1105 / tpc.013052.

Buensuceso C., de Virgilio M., and Shattil S.J. Detection of integrin alpha IIbbeta 3 clustering in living cells.J Biol Chem. 2003 278:15217-24. Doi: 10.1074/jbc.M213234200.

Chiesa A., Rapizzi E., Tosello V., Pinton P., de Virgilio M., Fogarty K.E., and Rizzuto R. Recombinant aequorin and green fluorescent protein as valuable tools in the study of cell signalling. Biochem J. 2001 355:1-12. Doi: 10.1042/0264-6021:3550001 .

de Virgilio M., Kitzmuller C., Schwaiger E., Klein M, Kreibich G., and Ivessa N.E. Degradation of a short-lived glycoprotein from the lumen of the endoplasmic reticulum: the role of N-linked glycans and the unfolded protein response. Mol Biol Cell. 1999 10:4059-73.Doi: 10.1091 / mbc.10.12.4059.

Frigerio L., de Virgilio M., Prada A., Faoro F., and Vitale A. Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide.Plant Cell. 1998 10:1031-42. Doi:

de Virgilio M., Weninger H., and Ivessa N.E. Ubiquitination is required for the retro-translocation of a short-lived luminal endoplasmic reticulum glycoprotein to the cytosol for degradation by the proteasome. J Biol Chem. 1998 273: 734-43. Doi: 10.1074 / jbc.273.16.9734

Pedrazzini E., Giovinazzo G., Bielli A., de Virgilio M., Frigerio L., Pesca M., Faoro F., Bollini R., Cerotti A., and Vitale A.Protein quality control along the route to the plant vacuole. Plant Cell 1997 9:1869-80. Doi: 10.1105 / tpc.9.10.1869

Ceci L.R. Spoto N., de Virgilio M., Gallerani R. The gene coding for the mustard trypsin inhibitor-2 is discontinuous and wound-inducible. FEBS Lett.1995 363: 179-81. Doi: 10.1016/0014-5793(95)00386-n.


Selected Publications

(full list available at CNR People)

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