Anna Valenti

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POSITION

Permanent position as Researcher at Institute of Biosciences and BioResources- National Research Council of Italy 


WORK EXPERIENCE

dates (12/2012 - to now) Permanent position as researcher

Institute of Biosciences and BioResources- National Research Council of Italy 


▪ Structure and function of proteins and enzymes involved in DNA metabolism in mesophilic and thermophilic microorganisms. Mechanisms of genome stability and repair in model organisms. Nanopores application to study DNA-protein interaction and DNA structure.

Sector Molecular Biology and Biochemistry

dates (03/2012 - 12/2012) Temporary position as researcher

Institute of Protein Biochemistry- National Research Council of Italy 


▪ Structure and function of proteins and enzymes involved in DNA metabolism in mesophilic and thermophilic microorganisms

Sector Molecular Biology and Biochemistry

dates (10/2008 - 01/2012) Post-doctoral fellow

Institute of Protein Biochemistry- National Research Council of Italy 


▪ Structure and function of proteins and enzymes involved in DNA metabolism (topoisomerases, helicases, DNA binding proteins, translesion DNA polymerases)

Sector Molecular Biology and Biochemistry

dates (10/2008 - 01/2012) Research fellow and PhD student

Institute of Protein Biochemistry- National Research Council of Italy 


▪ Mechanisms of genome stability and repair in microorganisms

Business or sector Molecular Biology and Biochemistry

EDUCATION AND TRAINING

dates (from 11/2004 - to 11/2007) PhD degree in Applied Biology

Date of graduation 07/02/2008

Institution: University of Naples “Federico II”

Title of Thesis: " Risposta UV in Sulfolobus solfataricus: regolazione della girasi inversa da danno al DNA"

dates (from 11/1996 - to 03/2004) Degree in Biology

Date of graduation 29/03/2004

Institution University of Naples “Federico II”

Title of Thesis: "La girasi inversa dell’Archeon termofilo Sulfolobus solfataricus"

dates (from 09/1991 - to 06/1996) High school diploma

Date of graduation 06/1996

Liceo Scientifico- A. Romita- Campobasso

Organisational / managerial skills/leadership

currently Principal investigator of Research Unit of reasearch project FIRB- Ministero Italiano dell’Università e Ricerca

Technical skills and competence

-Purification, biochemical analysis and engineering of proteins and enzymes involved in DNA metabolism (topoisomerases, helicases, DNA binding proteins, DNA polymerases);


-Immobilization of protein on nanopore material

-Production of DNA substrates with specific structure and topology


-Analysis of DNA topology

-In vivo and in vitro protein-protein interaction techniques (co-immunoprecipitation, pull-down, south-western blot, FRET, Surface plasmon resonance)

- In vivo and in vitro DNA-protein interaction techniques (EMSA, footprinting, crosslinking, chromatin immunoprecipitation)
- qualitative and quantitative gene expression analysis (western blot, northern blot, real-time RT-PCR)


- manipulation of bacteria, yeast and nematodes

PUBLICATIONS

Visone V., Vettone A., Serpe M., VALENTI A., Perugino G., Rossi M. and Maria Ciaramella. (2014) Chromatin structure and dynamics in hot environment: architectural proteins and DNA topoisomerases of thermophilic archaea. Review. INTERNATION JOURNAL OF MOLECULAR SCIENCES in press

Vettone A., Perugino G, Rossi M, VALENTI A, Ciaramella M. Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase. (2014) Review Extremophiles. Epub ahead of print

Jamroze A, Perugino G, VALENTI A, Rashid N, Rossi M, Akhtar M, Ciaramella M. (2013) The reverse gyrase from Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities. THE JOURNAL OF BIOLOGICAL CHEMISTRY

VALENTI A., De Felice M, Perugino G, Bizard A, Nadal M, Rossi M, Ciaramella M. (2012). Synergic and opposing activities of thermophilic RecQ-like helicase and Topoisomerase 3 in Holliday junction processing and replication fork stabilization. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

Perugino G, Vettone A, Illiano G, VALENTI A., Ferrara MC, Rossi M, Ciaramella M. (2012). Activity and Regulation of Archaeal DNA Alkyltransferase: conserved protein involved in repair of DNA alkylation damage. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

VALENTI A., Perugino G, Rossi M, Ciaramella M. (2011). Positive supercoiling in thermophiles and mesophiles: of the good and evil. BIOCHEMICAL SOCIETY TRANSACTIONS, ISSN: 0300-5127

VALENTI A., Perugino G, Varriale A, D’Auria S, Rossi M, Ciaramella M. (2010). The archaeal topoisomerase reverse gyrase is a helix-destabilizing protein that unwinds four-way DNA junctions. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

VALENTI A., Perugino G, Nohmi T, Rossi M, Ciaramella M. (2009). Inhibition of translesion DNA polymerase by archaeal reverse gyrase. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Perugino G, VALENTI A., D’amaro A, Rossi M, Ciaramella M. (2009). Reverse gyrase and genome stability in hyperthermophilic organisms. BIOCHEMICAL SOCIETY TRANSACTIONS, ISSN: 0300-5127

D’Amaro A, VALENTI A., Napoli A, Rossi M, Ciaramella M. (2008). The prefoldin of the crenarchaeon Sulfolobus solfataricus. PROTEIN AND PEPTIDE LETTERS, ISSN: 0929-8665

VALENTI A., Perugino G, D’Amaro A, Cacace A, Napoli A, Rossi M, Ciaramella M. (2008). Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Romano V, Napoli A, Salerno V, VALENTI A., Rossi M, Ciaramella M. (2007). Lack of strand-specific repair of UV-induced DNA lesions in three genes of the archaeon Sulfolobus solfataricus. JOURNAL OF MOLECULAR BIOLOGY, ISSN: 0022-2836

VALENTI A., Napoli A, di Simone C, Ferrara MC, Romano V, Rossi M and Ciaramella M (2006). Reverse gyrase and DNA damage response in thermophilic Archaea . ITALIAN JOURNAL OF BIOCHEMISTRY, ISSN: 0021-2938

D’Amaro A, VALENTI A., Napoli A, Rossi M and Ciaramella M (2006). Characterization of a thermophilic molecular chaperone: The prefoldin of the crenarchaeon Sulfolobus solfataricus. ITALIAN JOURNAL OF BIOCHEMISTRY, ISSN: 0021-2938

VALENTI A., Napoli A, Ferrara MC, Nadal M, Rossi M, Ciaramella M. (2006). Selective degradation of reverse gyrase and DNA fragmentation induced by alkylating agent in the archaeon Sulfolobus solfataricus. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Napoli A, VALENTI A., Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M. (2005). Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Napoli A, VALENTI A., Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M. (2004). Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

PROJECTS

-FIRB-Futurio in Ricerca (2013-2016): Materiali Nanoporosi: lavagnE autoasseMblAnti per lo sTudio della struttura e delle Interazioni del DNA (NEMATIC). PI of Research Unit: Anna Valenti

-TELETHON (2012-2014): New pharmacological targets in Fanconi Anemia. PI of Research Unit: Maria Ciaramella

-AIRC (2011-2013): Cisplatin sensitivity/tolerance/resistance genetic pathways in hereditary cancer predisposition. PI of Research Unit: Maria Ciaramella

Selected Publications

(full list available at CNR People)

In vivo analysis of FANCD2 recruitment at meiotic DNA breaks in Caenorhabditis elegans

Germoglio M, Valenti A, Gallo I, Forenza C, Santonicola P, Silva N, Adamo A

Year: 2020

A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures

Mattossovich R, Merlo R, Fontana A, d’ippolito G, Terns MP, Watts EA, Valenti A, Perugino G

Year: 2020

O6-alkylguanine-DNA Alkyltransferases in Microbes Living on the Edge: From Stability to Applicability

Mattossovich R, Merlo R, Miggiano R, Valenti A, Perugino G

Year: 2020

The SNAP- tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate

Merlo R, Caprioglio D, Cillo M, Valenti A, Mattossovich R, Morrone C, Massarotti A, Rossi F, Miggiano R, Leonardi A, Minassi A, Perugino G

Year: 2020

Topoisomerases inhibition and DNA binding mode of daunomycin-oligoarginine conjugate

Visone V, Szabó I, Perugino G, Hudecz F, Bánóczi Z, Valenti A

Year: 2020

New insights into structural and functional roles of Indole-3-acetic acid (IAA): Changes in DNA Topology and gene expression in Bacteria

Defez R, Valenti A, Andreozzi A, Romano S, Ciaramella M, Pesaresi P, Forlani S, Bianco C

Year: 2019

Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling- protein-tag system (ASL tag )

Del Prete S, Merlo R, Valenti A, Mattossovich R, Rossi M, Carginale V, Supuran CT, Perugino G, Capasso C

Year: 2019

An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane

Merlo R, Del Prete S, Valenti A, Mattossovich R, Carginale V, Supuran CT, Capasso C, Perugino G

Year: 2019

The DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) of Caenorhabditis elegans is involved in meiosis and early development under physiological conditions

Serpe M, Forenza C, Adamo A, Russo N, Perugino G, Ciaramella M, Valenti A

Year: 2019

Structure and properties of DNA molecules over the full range of biologically relevant supercoiling states

Bettotti P, Visone V, Lunelli L, Perugino G, Ciaramella M, Valenti A

Year: 2018

Crystal structure of a thermophilic O⁶-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe

Rossi F, Morrone C, Massarotti A, Ferraris DM, Valenti A, Perugino G, Miggiano R

Year: 2018

Every OGT is illuminated… by fluorescent and synchrotron lights

Miggiano R, Valenti A, Rossi F, Rizzi M, Perugino G, Ciaramella M

Year: 2017

Interdomain interactions rearrangements control the reaction steps of a thermostable DNA alkyltransferase

Morrone C, Miggiano R, Serpe M, Massarotti A, Valenti A, Del Monaco G, Rossi M, Rossi F, Rizzi M, Perugino G, Ciaramella M

Year: 2017

In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag

Visone V, Han W, Perugino G, del Monaco G, She Q, Rossi M, Valenti A, Ciaramella M

Year: 2017

A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering

Vettone A, Serpe M, Hidalgo A, Berenguer J, Del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G

Year: 2016

Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein

Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M

Year: 2015

Massively Systematic Transcript End Readout, “MASTER”: Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields

Vvedenskaya I, Zhang Y, Goldman S, Valenti A, Visone V, Taylor D, Ebright R, Nickels B

Year: 2015

Genome caretakers from hyperthermophilic crenarchaea

Ciaramella M, Perugino G, Rossi M, Serpe M, Valenti A, Vettone A, Visone V

Year: 2014

The reverse gyrase form Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities

Jamroze A, Perugino G, Valenti A, Rashid N, Rossi M, Akhtar M, Ciaramella M

Year: 2014

Activity and regulation of archeal DNA alkyltransferase involved in repair of DNA alkylation damage

Perugino G, Serpe M, Vettone A, Valenti A, Ciaramella M

Year: 2014

A novel flexible protein tag for thermophiles: the Sulfolobus solfataricus DNA-alkyltransferase protein

Perugino G, Vettone A, Serpe M, Valenti A, Berenguer JC, Huertas AH, Rossi M, Ciaramella M

Year: 2014

Detection of DNA-protein interaction and structural modifications by nanopore-based microarrays.

Valenti A, Bettotti P, Ciaramella M, Chiari M, Cretich M, Gagni P, Perugino G, Vettone A, Visone V

Year: 2014

Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase

Vettone A, Perugino G, Rossi M, Valenti A, Ciaramella M

Year: 2014

A new analytical approach to study DNA protein interactions and modifications

Visone V, Bettotti P, Ciaramella M, Chiari M, Cretich M, Gagni P, Perugino G, Valenti A, Vettone A

Year: 2014

Chromatin structure and dynamics in hot environment: architectural proteins and DNA topoisomerases of thermophilic archaea

Visone V, Vettone A, Serpe M, Valenti A, Perugino G, Rossi M, Ciaramella M

Year: 2014