Anna Valenti
Role: Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132496
E-mail: anna.valenti@ibbr.cnr.it
POSITION
Permanent position as Researcher at Institute of Biosciences and BioResources- National Research Council of Italy
WORK EXPERIENCE
dates (12/2012 - to now) Permanent position as researcher
Institute of Biosciences and BioResources- National Research Council of Italy
▪ Structure and function of proteins and enzymes involved in DNA metabolism in mesophilic and thermophilic microorganisms. Mechanisms of genome stability and repair in model organisms. Nanopores application to study DNA-protein interaction and DNA structure.
Sector Molecular Biology and Biochemistry
dates (03/2012 - 12/2012) Temporary position as researcher
Institute of Protein Biochemistry- National Research Council of Italy
▪ Structure and function of proteins and enzymes involved in DNA metabolism in mesophilic and thermophilic microorganisms
Sector Molecular Biology and Biochemistry
dates (10/2008 - 01/2012) Post-doctoral fellow
Institute of Protein Biochemistry- National Research Council of Italy
▪ Structure and function of proteins and enzymes involved in DNA metabolism (topoisomerases, helicases, DNA binding proteins, translesion DNA polymerases)
Sector Molecular Biology and Biochemistry
dates (10/2008 - 01/2012) Research fellow and PhD student
Institute of Protein Biochemistry- National Research Council of Italy
▪ Mechanisms of genome stability and repair in microorganisms
Business or sector Molecular Biology and Biochemistry
EDUCATION AND TRAINING
dates (from 11/2004 - to 11/2007) PhD degree in Applied Biology
Date of graduation 07/02/2008
Institution: University of Naples “Federico II”
Title of Thesis: " Risposta UV in Sulfolobus solfataricus: regolazione della girasi inversa da danno al DNA"
dates (from 11/1996 - to 03/2004) Degree in Biology
Date of graduation 29/03/2004
Institution University of Naples “Federico II”
Title of Thesis: "La girasi inversa dell’Archeon termofilo Sulfolobus solfataricus"
dates (from 09/1991 - to 06/1996) High school diploma
Date of graduation 06/1996
Liceo Scientifico- A. Romita- Campobasso
Organisational / managerial skills/leadership
currently Principal investigator of Research Unit of reasearch project FIRB- Ministero Italiano dell’Università e Ricerca
Technical skills and competence
-Purification, biochemical analysis and engineering of proteins and enzymes involved in DNA metabolism (topoisomerases, helicases, DNA binding proteins, DNA polymerases);
-Immobilization of protein on nanopore material
-Production of DNA substrates with specific structure and topology
-Analysis of DNA topology
-In vivo and in vitro protein-protein interaction techniques (co-immunoprecipitation, pull-down, south-western blot, FRET, Surface plasmon resonance)
- In vivo and in vitro DNA-protein interaction techniques (EMSA, footprinting, crosslinking, chromatin immunoprecipitation) - qualitative and quantitative gene expression analysis (western blot, northern blot, real-time RT-PCR)
- manipulation of bacteria, yeast and nematodes
PUBLICATIONS
Visone V., Vettone A., Serpe M., VALENTI A., Perugino G., Rossi M. and Maria Ciaramella. (2014) Chromatin structure and dynamics in hot environment: architectural proteins and DNA topoisomerases of thermophilic archaea. Review. INTERNATION JOURNAL OF MOLECULAR SCIENCES in press
Vettone A., Perugino G, Rossi M, VALENTI A, Ciaramella M. Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase. (2014) Review Extremophiles. Epub ahead of print
Jamroze A, Perugino G, VALENTI A, Rashid N, Rossi M, Akhtar M, Ciaramella M. (2013) The reverse gyrase from Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities. THE JOURNAL OF BIOLOGICAL CHEMISTRY
VALENTI A., De Felice M, Perugino G, Bizard A, Nadal M, Rossi M, Ciaramella M. (2012). Synergic and opposing activities of thermophilic RecQ-like helicase and Topoisomerase 3 in Holliday junction processing and replication fork stabilization. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258
Perugino G, Vettone A, Illiano G, VALENTI A., Ferrara MC, Rossi M, Ciaramella M. (2012). Activity and Regulation of Archaeal DNA Alkyltransferase: conserved protein involved in repair of DNA alkylation damage. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258
VALENTI A., Perugino G, Rossi M, Ciaramella M. (2011). Positive supercoiling in thermophiles and mesophiles: of the good and evil. BIOCHEMICAL SOCIETY TRANSACTIONS, ISSN: 0300-5127
VALENTI A., Perugino G, Varriale A, D’Auria S, Rossi M, Ciaramella M. (2010). The archaeal topoisomerase reverse gyrase is a helix-destabilizing protein that unwinds four-way DNA junctions. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258
VALENTI A., Perugino G, Nohmi T, Rossi M, Ciaramella M. (2009). Inhibition of translesion DNA polymerase by archaeal reverse gyrase. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048
Perugino G, VALENTI A., D’amaro A, Rossi M, Ciaramella M. (2009). Reverse gyrase and genome stability in hyperthermophilic organisms. BIOCHEMICAL SOCIETY TRANSACTIONS, ISSN: 0300-5127
D’Amaro A, VALENTI A., Napoli A, Rossi M, Ciaramella M. (2008). The prefoldin of the crenarchaeon Sulfolobus solfataricus. PROTEIN AND PEPTIDE LETTERS, ISSN: 0929-8665
VALENTI A., Perugino G, D’Amaro A, Cacace A, Napoli A, Rossi M, Ciaramella M. (2008). Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048
Romano V, Napoli A, Salerno V, VALENTI A., Rossi M, Ciaramella M. (2007). Lack of strand-specific repair of UV-induced DNA lesions in three genes of the archaeon Sulfolobus solfataricus. JOURNAL OF MOLECULAR BIOLOGY, ISSN: 0022-2836
VALENTI A., Napoli A, di Simone C, Ferrara MC, Romano V, Rossi M and Ciaramella M (2006). Reverse gyrase and DNA damage response in thermophilic Archaea . ITALIAN JOURNAL OF BIOCHEMISTRY, ISSN: 0021-2938
D’Amaro A, VALENTI A., Napoli A, Rossi M and Ciaramella M (2006). Characterization of a thermophilic molecular chaperone: The prefoldin of the crenarchaeon Sulfolobus solfataricus. ITALIAN JOURNAL OF BIOCHEMISTRY, ISSN: 0021-2938
VALENTI A., Napoli A, Ferrara MC, Nadal M, Rossi M, Ciaramella M. (2006). Selective degradation of reverse gyrase and DNA fragmentation induced by alkylating agent in the archaeon Sulfolobus solfataricus. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048
Napoli A, VALENTI A., Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M. (2005). Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048
Napoli A, VALENTI A., Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M. (2004). Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258
PROJECTS
-FIRB-Futurio in Ricerca (2013-2016): Materiali Nanoporosi: lavagnE autoasseMblAnti per lo sTudio della struttura e delle Interazioni del DNA (NEMATIC). PI of Research Unit: Anna Valenti
-TELETHON (2012-2014): New pharmacological targets in Fanconi Anemia. PI of Research Unit: Maria Ciaramella
-AIRC (2011-2013): Cisplatin sensitivity/tolerance/resistance genetic pathways in hereditary cancer predisposition. PI of Research Unit: Maria Ciaramella
Selected Publications
(full list available at CNR People)
In vivo analysis of FANCD2 recruitment at meiotic DNA breaks in Caenorhabditis elegans
Germoglio M, Valenti A, Gallo I, Forenza C, Santonicola P, Silva N, Adamo A
Year: 2020
A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures
Mattossovich R, Merlo R, Fontana A, d’ippolito G, Terns MP, Watts EA, Valenti A, Perugino G
Year: 2020
O6-alkylguanine-DNA Alkyltransferases in Microbes Living on the Edge: From Stability to Applicability
Mattossovich R, Merlo R, Miggiano R, Valenti A, Perugino G
Year: 2020
The SNAP- tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate
Merlo R, Caprioglio D, Cillo M, Valenti A, Mattossovich R, Morrone C, Massarotti A, Rossi F, Miggiano R, Leonardi A, Minassi A, Perugino G
Year: 2020
Topoisomerases inhibition and DNA binding mode of daunomycin-oligoarginine conjugate
Visone V, Szabó I, Perugino G, Hudecz F, Bánóczi Z, Valenti A
Year: 2020
New insights into structural and functional roles of Indole-3-acetic acid (IAA): Changes in DNA Topology and gene expression in Bacteria
Defez R, Valenti A, Andreozzi A, Romano S, Ciaramella M, Pesaresi P, Forlani S, Bianco C
Year: 2019
Thermostability enhancement of the α-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling- protein-tag system (ASL tag )
Del Prete S, Merlo R, Valenti A, Mattossovich R, Rossi M, Carginale V, Supuran CT, Perugino G, Capasso C
Year: 2019
An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane
Merlo R, Del Prete S, Valenti A, Mattossovich R, Carginale V, Supuran CT, Capasso C, Perugino G
Year: 2019
The DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) of Caenorhabditis elegans is involved in meiosis and early development under physiological conditions
Serpe M, Forenza C, Adamo A, Russo N, Perugino G, Ciaramella M, Valenti A
Year: 2019
Structure and properties of DNA molecules over the full range of biologically relevant supercoiling states
Bettotti P, Visone V, Lunelli L, Perugino G, Ciaramella M, Valenti A
Year: 2018
Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe
Rossi F, Morrone C, Massarotti A, Ferraris DM, Valenti A, Perugino G, Miggiano R
Year: 2018
Every OGT is illuminated… by fluorescent and synchrotron lights
Miggiano R, Valenti A, Rossi F, Rizzi M, Perugino G, Ciaramella M
Year: 2017
Interdomain interactions rearrangements control the reaction steps of a thermostable DNA alkyltransferase
Morrone C, Miggiano R, Serpe M, Massarotti A, Valenti A, Del Monaco G, Rossi M, Rossi F, Rizzi M, Perugino G, Ciaramella M
Year: 2017
In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag
Visone V, Han W, Perugino G, del Monaco G, She Q, Rossi M, Valenti A, Ciaramella M
Year: 2017
A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA- alkyl-transferase by protein engineering
Vettone A, Serpe M, Hidalgo A, Berenguer J, Del Monaco G, Valenti A, Rossi M, Ciaramella M, Perugino G
Year: 2016
Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein
Perugino G, Miggiano R, Serpe M, Vettone A, Valenti A, Lahiri S, Rossi F, Rossi M, Rizzi M, Ciaramella M
Year: 2015
Massively Systematic Transcript End Readout, “MASTER”: Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields
Vvedenskaya I, Zhang Y, Goldman S, Valenti A, Visone V, Taylor D, Ebright R, Nickels B
Year: 2015
Genome caretakers from hyperthermophilic crenarchaea
Ciaramella M, Perugino G, Rossi M, Serpe M, Valenti A, Vettone A, Visone V
Year: 2014
The reverse gyrase form Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities
Jamroze A, Perugino G, Valenti A, Rashid N, Rossi M, Akhtar M, Ciaramella M
Year: 2014
Activity and regulation of archeal DNA alkyltransferase involved in repair of DNA alkylation damage
Perugino G, Serpe M, Vettone A, Valenti A, Ciaramella M
Year: 2014
A novel flexible protein tag for thermophiles: the Sulfolobus solfataricus DNA-alkyltransferase protein
Perugino G, Vettone A, Serpe M, Valenti A, Berenguer JC, Huertas AH, Rossi M, Ciaramella M
Year: 2014
Detection of DNA-protein interaction and structural modifications by nanopore-based microarrays.
Valenti A, Bettotti P, Ciaramella M, Chiari M, Cretich M, Gagni P, Perugino G, Vettone A, Visone V
Year: 2014
Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase
Vettone A, Perugino G, Rossi M, Valenti A, Ciaramella M
Year: 2014
A new analytical approach to study DNA protein interactions and modifications
Visone V, Bettotti P, Ciaramella M, Chiari M, Cretich M, Gagni P, Perugino G, Valenti A, Vettone A
Year: 2014
Chromatin structure and dynamics in hot environment: architectural proteins and DNA topoisomerases of thermophilic archaea
Visone V, Vettone A, Serpe M, Valenti A, Perugino G, Rossi M, Ciaramella M
Year: 2014