TuberID

Taxon

Species ID

Species Name

Tuber suave

Clade

Aestivum

Index Fungorum Record

842206

MycoBank ID

590499

Fungal Names ID

842206

Catalogue of Life Record

9ZGX8

GBIF Record

11839716

Synonyms

Edible

Authorship

Pacioni & M. Leonardi

Author Names

Pacioni G., Leonardi M.

Reference

J. Fungi 1(12, no. 1090)

First Description (Year)

2021

Ascocarp

Odour

Complex, intense, and pleasant

Ascocarp Form

Ascocarp Size (cm)

Peridium

External Morphology (Peridium)

warty, with flat or excavate base

Peridium Color

black

Wart Morphology

flat pyramidal with 4-6 side, without orizontal striae

Wart Size (cm)

2-3

Gleba

Gleba Color at Maturity

deep brown

Gleba Aspect

marbled with white, meandering, rather wide sterile veins

Spores

Spore Form

ellipsoidal, sometimes globose or slightly elongated

Spore Minimum Length (μm)

Spore Maximum Length (μm)

Spore Minimum Diameter (μm)

Spore Maximum Diameter (μm)

Spore color

yellowish or burnt orange

Spore ornamentation

reticulum of complete polygonal alveolae with 4-6 sides

Spore ornamentation height (µm)

2.0–7.0

Spore ornamentation diameter (µm)

2.0–10

Mycorrhiza (ECM)

ECM Form

ECM color (young)

ECM color (mature)

Cystidia form

Cystidia color

Cystidia Length (µm)

Cystidia Basal Diameter (µm)

Cystidia Position

Mantle Cells Form

Mantle cell density
(in a 20x20µm square)

Hosts

Host #5

Host Latin Name

Quercus pubescens

Catalogue of Life Record

4R5VW

GBIF Record

2881283

Host #7

Host Latin Name

Quercus ilex

Catalogue of Life Record

793PC

GBIF Record

2879098

Host #28

Host Latin Name

Quercus coccifera

Catalogue of Life Record

4R4M7

GBIF Record

2879175

Molecular Identification Methods

Method #16 - Marker: ITS

Forward Primer

(CladeIII_f) ACTTGGTAAACTGAAGCAGAC

Melting Temp (°C)

Reverse Primer

(CladeIII_r) CTTCACGCCGATTACAACG

PCR Product Size (bps)

365

MgCl₂ Conc.

Additives in mixture

BSA (0.8 mg/ml)

PCR cycles

95 °C 6 min, 40 cycles (94 °C 45 s, 53 °C 30 s, 72 °C 45 s), final extension 72 °C 7 min.

Reference

Leonardi M., Salvi D., Iotti M., Rana G. L., Paz-Conde A., Pacioni G. (2021). Multilocus phylogeography of the Tuber mesentericum complex unearths three highly divergent cryptic species. Journal of Fungi, vol. 7, pp. 1090. - doi: https://doi.org/10.3390/jof7121090

**Database for morphological and molecular identification of Tuber species**

Tuber suave

Taxon

Ascocarp

Peridium

Gleba

Spores

Mycorrhiza (ECM)

Hosts

Host #5

Host #7

Host #28

Molecular Identification Methods

Method #16 - Marker: ITS

Contents