TuberID

Database for morphological and molecular identification of Tuber species

Tuber bituminatum

*
Ascocarp
*
Spores

(Click on the above pictures to enlarge them)

Taxon

Species ID
6
Species Name
Tuber bituminatum
Clade
Aestivum
Index Fungorum Record
196523
MycoBank ID
233831
Fungal Names ID
196523
Catalogue of Life Record
59HXJ
GBIF Record
5258474
Synonyms
-
Edible
Y
Authorship
Berk. & Broome
Author Names
Berkeley M.J, Broome C.E.
Reference
Ann. Mag. nat. Hist., Ser. 1 18: 81
First Description (Year)
1851

Ascocarp

Odour
From phenolic to very pleasant
Ascocarp Form
-
Ascocarp Size (cm)
-

Peridium

External Morphology (Peridium)
warty, sometimes with excavate base
Peridium Color
black
Wart Morphology
flat pyramidal with 4-6 sides
Wart Size (cm)
3

Gleba

Gleba Color at Maturity
brown to dark orange
Gleba Aspect
marbled with whitish sterile veins, meandering or radiating from the base

Spores

Spore Form
ellipsoidal, sometimes globose or fusiform
Spore Minimum Length (μm)
26
Spore Maximum Length (μm)
54
Spore Minimum Diameter (μm)
29
Spore Maximum Diameter (μm)
70
Spore color
yellow to yellow-orange
Spore ornamentation
pseudoreticula with incomplete and irregular meshes
Spore ornamentation height (µm)
2.0–9.5
Spore ornamentation diameter (µm)
2.0–10

Mycorrhiza (ECM)

ECM Form
-
ECM color (young)
-
ECM color (mature)
-
Cystidia form
-
Cystidia color
-
Cystidia Length (µm)
-
Cystidia Basal Diameter (µm)
-
Cystidia Position
-
Mantle Cells Form
-
Mantle cell density
(in a 20x20µm square)
-

Hosts

Host #1
Host Latin Name
Quercus cerris
Catalogue of Life Record
4R4KH
GBIF Record
2880580
Host #2
Host Latin Name
Quercus robur
Catalogue of Life Record
4R5YN
GBIF Record
2878688
Host #3
Host Latin Name
Corylus avellana
Catalogue of Life Record
YQ55
GBIF Record
2875979
Host #4
Host Latin Name
Fagus sylvatica
Catalogue of Life Record
3DSK5
GBIF Record
2882316
Host #5
Host Latin Name
Quercus pubescens
Catalogue of Life Record
4R5VW
GBIF Record
2881283
Host #7
Host Latin Name
Quercus ilex
Catalogue of Life Record
793PC
GBIF Record
2879098
Host #9
Host Latin Name
Ostrya carpinifolia
Catalogue of Life Record
6TCXD
GBIF Record
5332305
Host #10
Host Latin Name
Tilia cordata
Catalogue of Life Record
56WPP
GBIF Record
3152047
Host #11
Host Latin Name
Tilia platyphyllos
Catalogue of Life Record
7CC2T
GBIF Record
3152048
Host #12
Host Latin Name
Pinus nigra
Catalogue of Life Record
4J2D5
GBIF Record
5284809
Host #13
Host Latin Name
Pinus pinea
Catalogue of Life Record
77KSK
GBIF Record
5285165
Host #14
Host Latin Name
Pinus sylvestris
Catalogue of Life Record
4J2J5
GBIF Record
5285637
Host #15
Host Latin Name
Pinus halepensis
Catalogue of Life Record
4J27Y
GBIF Record
5285604

Molecular Identification Methods

Method #15 - Marker: ITS
Forward Primer
(CladeII_f) CGTGAACACACTTTGGACA
Melting Temp (°C)
53
Reverse Primer
(CladeII_r) CTGCCTTACGCTAATTACAAC
PCR Product Size (bps)
372
MgCl2 Conc.
-
Additives in mixture
BSA (0.8 mg/ml)
PCR cycles
95 °C 6 min, 40 cycles (94 °C 45 s, 53 °C 30 s, 72 °C 45 s), final extension 72 °C 7 min.
Reference
Leonardi M., Salvi D., Iotti M., Rana G. L., Paz-Conde A., Pacioni G. (2021). Multilocus phylogeography of the Tuber mesentericum complex unearths three highly divergent cryptic species. Journal of Fungi, vol. 7, pp. 1090. - doi: https://doi.org/10.3390/jof7121090

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This project has received funding from European Union's Horizon 2020 research and innovation program under Grant Agreement #101007623.

Contacts:

Andrea Rubini, CNR-IBBR, Perugia (andrea.rubini@cnr.it)
Claudia Riccioni, CNR-IBBR, Perugia (claudia.riccioni@cnr.it)
Beatrice Belfiori, CNR-IBBR, Perugia (beatrice.belfiori@cnr.it)

Web Manager:

Gabriele Bucci, CNR-IBBR, Sesto Fiorentino, FI (gabriele.bucci@cnr.it)