TuberID

Taxon

Species ID

Species Name

Tuber mesentericum

Clade

Aestivum

Index Fungorum Record

180551

MycoBank ID

229865

Fungal Names ID

180551

Catalogue of Life Record

59HZZ

GBIF Record

5258471

Synonyms

Edible

Authorship

Vittad.

Author Names

Vittadini C.

Reference

Monogr. Tuberac. (Milano): 44

First Description (Year)

1831

Ascocarp

Odour

Pleasant, like solvent (similar to iodine)

Ascocarp Form

generally subglobose

Ascocarp Size (cm)

2-3

Peridium

External Morphology (Peridium)

warty, with uniform warts, with excavate base

Peridium Color

black

Wart Morphology

flat pyramidal warts with 3-6 sides, with fine horizontal striae.

Wart Size (cm)

Gleba

Gleba Color at Maturity

brown to dark-orange or dark red.

Gleba Aspect

marbled with whitish sterile veins, meandriform and often radiating from the base

Spores

Spore Form

ellipsoidal, rarely globose or elongated

Spore Minimum Length (μm)

Spore Maximum Length (μm)

Spore Minimum Diameter (μm)

Spore Maximum Diameter (μm)

Spore color

yellow to yellow-orange

Spore ornamentation

reticulated, with regular meshes

Spore ornamentation height (µm)

2-8

Spore ornamentation diameter (µm)

1.5-10

Mycorrhiza (ECM)

ECM Form

cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)

ECM color (young)

yellow-ochre

ECM color (mature)

brown

Cystidia form

very long, not ramified, less curly than in T. aestivum, forming a web of mycelium around the tip

Cystidia color

yellow-ochre

Cystidia Length (µm)

200-800

Cystidia Basal Diameter (µm)

2-4

Cystidia Position

Method #14 - Marker: ITS

Forward Primer

(CladeI_f) ATAACGAGAAGTCTGAACC

Melting Temp (°C)

Reverse Primer

(CladeI_r) CTGCTCTACGCTTATCACA

PCR Product Size (bps)

357

MgCl₂ Conc.

Additives in mixture

BSA (0.8 mg/ml)

PCR cycles

95 °C 6 min, 40 cycles (94 °C 45 s, 53 °C 30 s, 72 °C 45 s), final extension 72 °C 7 min.

Reference

Leonardi M., Salvi D., Iotti M., Rana G. L., Paz-Conde A., Pacioni G. (2021). Multilocus phylogeography of the Tuber mesentericum complex unearths three highly divergent cryptic species. Journal of Fungi, vol. 7, pp. 1090. - doi: https://doi.org/10.3390/jof7121090

**Database for morphological and molecular identification of Tuber species**

Tuber mesentericum

Taxon

Ascocarp

Peridium

Gleba

Spores

Mycorrhiza (ECM)

Hosts

Host #1

Host #2

Host #3

Host #4

Host #5

Host #7

Host #9

Host #10

Host #11

Host #12

Host #13

Host #14

Host #15

Molecular Identification Methods

Method #14 - Marker: ITS

Contents