TuberID

Database for morphological and molecular identification of Tuber species

Tuber aestivum var. uncinatum

(Click on the above pictures to enlarge them)

Taxon

Species ID
4
Species Name
Tuber aestivum var. uncinatum
Clade
Aestivum
Index Fungorum Record
653555
MycoBank ID
431465
Fungal Names ID
318194
Catalogue of Life Record
TW4J9
GBIF Record
5499338
Synonyms
Tuber aestivum, Tuber aestivum f. uncinatum
Edible
Y
Authorship
(Chatin) I.R. Hall, P.K. Buchanan, Wang & Cole
Author Names
Chatin G.A., Hall I.R., Buchanan P.K., P.K., Wan Y., Cole
Reference
Edible and Poisonous Mushrooms (Christchurch)
First Description (Year)
1995

Ascocarp

Odour
Pleasant
Ascocarp Form
generally subglobose
Ascocarp Size (cm)
-

Peridium

External Morphology (Peridium)
Warty with big warts of very variable size
Peridium Color
black
Wart Morphology
big, hard, black warts,pyramidal and irregularly polygonal at the base, with evident horizontal striae in the surface
Wart Size (cm)
4-7

Gleba

Gleba Color at Maturity
ochre , hazel-brown to dark-brown
Gleba Aspect
fleshy and marbled, with thin, meandriform veins

Spores

Spore Form
ellipsoidal to subglobose
Spore Minimum Length (μm)
18
Spore Maximum Length (μm)
22
Spore Minimum Diameter (μm)
25
Spore Maximum Diameter (μm)
30
Spore color
yellow to light brown
Spore ornamentation
reticulate-alveolate with poligonal irregular meshes
Spore ornamentation height (µm)
3-5
Spore ornamentation diameter (µm)
4-10

Mycorrhiza (ECM)

ECM Form
-
ECM color (young)
-
ECM color (mature)
-
Cystidia form
-
Cystidia color
-
Cystidia Length (µm)
-
Cystidia Basal Diameter (µm)
-
Cystidia Position
-
Mantle Cells Form
-
Mantle cell density
(in a 20x20µm square)
-

Hosts

Host #1
Host Latin Name
Quercus cerris
Catalogue of Life Record
4R4KH
GBIF Record
2880580
Host #2
Host Latin Name
Quercus robur
Catalogue of Life Record
4R5YN
GBIF Record
2878688
Host #3
Host Latin Name
Corylus avellana
Catalogue of Life Record
YQ55
GBIF Record
2875979
Host #4
Host Latin Name
Fagus sylvatica
Catalogue of Life Record
3DSK5
GBIF Record
2882316
Host #5
Host Latin Name
Quercus pubescens
Catalogue of Life Record
4R5VW
GBIF Record
2881283
Host #7
Host Latin Name
Quercus ilex
Catalogue of Life Record
793PC
GBIF Record
2879098
Host #9
Host Latin Name
Ostrya carpinifolia
Catalogue of Life Record
6TCXD
GBIF Record
5332305
Host #10
Host Latin Name
Tilia cordata
Catalogue of Life Record
56WPP
GBIF Record
3152047
Host #11
Host Latin Name
Tilia platyphyllos
Catalogue of Life Record
7CC2T
GBIF Record
3152048
Host #12
Host Latin Name
Pinus nigra
Catalogue of Life Record
4J2D5
GBIF Record
5284809
Host #13
Host Latin Name
Pinus pinea
Catalogue of Life Record
77KSK
GBIF Record
5285165
Host #14
Host Latin Name
Pinus sylvestris
Catalogue of Life Record
4J2J5
GBIF Record
5285637
Host #15
Host Latin Name
Pinus halepensis
Catalogue of Life Record
4J27Y
GBIF Record
5285604

Molecular Identification Methods

Method #11 - Marker: ITS
Forward Primer
(UncI) TGGGCCGCCGAAAACTTG
Melting Temp (°C)
59
Reverse Primer
(UncII) CTGACGAGATGCCCCGGA
PCR Product Size (bps)
405
MgCl2 Conc.
-
Additives in mixture
-
PCR cycles
27 cycles: 95 °C 1 min, 59 °C 45 s,72 °C 45 s
Reference
Mello A, Cantisani A, Vizzini A, Bonfante P (2002). Genetic variability of Tuber uncinatum and its relatedness to other black truffles.. Environmental Microbiology, vol. 4, pp. 584-594. - doi: https://doi.org/10.1046/j.1462-2920.2002.00343.x
Method #13 - Marker: ITS
Forward Primer
(Tu1sekvF ) AGAGCACCAAACCACAG
Melting Temp (°C)
52,59,63.5
Reverse Primer
(Tu2sekvR) ACCACAGCGTCTACCAA
PCR Product Size (bps)
496-502
MgCl2 Conc.
5 mM
Additives in mixture
-
PCR cycles
95°C, 4min, 34 or 40* cycles (95°C, 60 or 40s*; 52, 59 or 63.5°C, 45 or 40s*, 72°C, 45 or 40s*), 72°C for 5min
Reference
Gryndler M, Hršelová H, Soukupová L, Streiblová E, Valda S, Borovička J, Gryndlerová H, Gažo J, Miko M (2011). Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers: molecular detection of Tuber aestivum. FEMS Microbiology Letters, vol. 318, pp. 84-91. - doi: https://doi.org/10.1111/j.1574-6968.2011.02243.x

© 2025 CNR-IBBR. All rights reserved.

All the material on these pages is released under the license Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

This project has received funding from European Union's Horizon 2020 research and innovation program under Grant Agreement #101007623.

Contacts:

Andrea Rubini, CNR-IBBR, Perugia (andrea.rubini@cnr.it)
Claudia Riccioni, CNR-IBBR, Perugia (claudia.riccioni@cnr.it)
Beatrice Belfiori, CNR-IBBR, Perugia (beatrice.belfiori@cnr.it)

Web Manager:

Gabriele Bucci, CNR-IBBR, Sesto Fiorentino, FI (gabriele.bucci@cnr.it)