TuberID

Taxon

Species ID

Species Name

Tuber aestivum

Clade

Aestivum

Index Fungorum Record

218597

MycoBank ID

159974

Fungal Names ID

218597

Catalogue of Life Record

TW4HM

GBIF Record

5258469

Synonyms

Tuber aestivum var. uncinatum, Tuber aestivum f. uncinatum

Edible

Authorship

Vittad.

Author Names

Vittadini C.

Reference

Monogr. Tuberac. (Milano): 44

First Description (Year)

1831

Ascocarp

Odour

Weak, mushroom like to more pleasant at maturity

Ascocarp Form

generally subglobose

Ascocarp Size (cm)

1-7

Peridium

External Morphology (Peridium)

Warty with big warts of very variable size

Peridium Color

black

Wart Morphology

big, hard, black warts,pyramidal and irregularly polygonal at the base, with evident horizontal striae in the surface

Wart Size (cm)

4-7

Gleba

Gleba Color at Maturity

white-ochre , hazel-brown to dark-brown

Gleba Aspect

fleshy and marbled, with thin, meandriform veins

Spores

Spore Form

ellipsoidal to subglobose

Spore Minimum Length (μm)

Spore Maximum Length (μm)

Spore Minimum Diameter (μm)

Spore Maximum Diameter (μm)

Spore color

yellow to light brown

Spore ornamentation

reticulate-alveolate with poligonal irregular meshes

Spore ornamentation height (µm)

3-5

Spore ornamentation diameter (µm)

4-10

Mycorrhiza (ECM)

ECM Form

cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)

ECM color (young)

yellow-ochre

ECM color (mature)

brown

Cystidia form

very long, not ramified, curly, forming a web of mycelium around the tip

Cystidia color

yellow-ochre

Cystidia Length (µm)

110-600

Cystidia Basal Diameter (µm)

2-4

Cystidia Position

Method #10 - Marker: ITS

Forward Primer

(UncI) TGGGCCGCCGAAAACTTG

Melting Temp (°C)

Reverse Primer

(UncII) CTGACGAGATGCCCCGGA

PCR Product Size (bps)

405

MgCl₂ Conc.

Additives in mixture

PCR cycles

27 cycles: 95 °C 1 min, 59 °C 45 s,72 °C 45 s

Reference

Mello A, Cantisani A, Vizzini A, Bonfante P (2002). Genetic variability of Tuber uncinatum and its relatedness to other black truffles.. Environmental Microbiology, vol. 4, pp. 584-594. - doi: https://doi.org/10.1046/j.1462-2920.2002.00343.x

Method #12 - Marker: ITS

Forward Primer

(Tu1sekvF ) AGAGCACCAAACCACAG

Melting Temp (°C)

52,59,63.5

Reverse Primer

(Tu2sekvR) ACCACAGCGTCTACCAA

PCR Product Size (bps)

496-502

MgCl₂ Conc.

5 mM

Additives in mixture

PCR cycles

95°C, 4min, 34 or 40* cycles (95°C, 60 or 40s*; 52, 59 or 63.5°C, 45 or 40s*, 72°C, 45 or 40s*), 72°C for 5min

Reference

Gryndler M, Hršelová H, Soukupová L, Streiblová E, Valda S, Borovička J, Gryndlerová H, Gažo J, Miko M (2011). Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers: molecular detection of Tuber aestivum. FEMS Microbiology Letters, vol. 318, pp. 84-91. - doi: https://doi.org/10.1111/j.1574-6968.2011.02243.x

**Database for morphological and molecular identification of Tuber species**

Tuber aestivum

Taxon

Ascocarp

Peridium

Gleba

Spores

Mycorrhiza (ECM)

Hosts

Host #1

Host #2

Host #3

Host #4

Host #5

Host #9

Host #10

Host #11

Host #12

Host #13

Host #14

Host #15

Molecular Identification Methods

Method #10 - Marker: ITS

Method #12 - Marker: ITS

Contents