TuberID

Taxon

Species ID

Species Name

Tuber macrosporum

Clade

Aestivum

Index Fungorum Record

190290

MycoBank ID

232265

Fungal Names ID

190290

Catalogue of Life Record

59HZQ

GBIF Record

5258460

Synonyms

Edible

Authorship

Vittad.

Author Names

Vittadini C.

Reference

Monogr. Tuberac. (Milano): 44

First Description (Year)

1831

Ascocarp

Odour

Intense, garlic-like, reminding of Tuber magnatum

Ascocarp Form

subglobose or irregular

Ascocarp Size (cm)

2-5

Peridium

External Morphology (Peridium)

Warty, warts distributed unevenly, sometimes smooth

Peridium Color

blackish, brown-reddish and with rusty fissures

Wart Morphology

small, flattened, iregularly polygonal, ridged

Wart Size (cm)

0.5-2

Gleba

Gleba Color at Maturity

grey brown, brown-lilac to purple-brown

Gleba Aspect

marbled with thick, interrupted white veins

Spores

Spore Form

ellipsoidal, very big

Spore Minimum Length (μm)

Spore Maximum Length (μm)

Spore Minimum Diameter (μm)

Spore Maximum Diameter (μm)

Spore color

yellow-brown

Spore ornamentation

alveolate, alveols with closed meshes

Spore ornamentation height (µm)

2-4

Spore ornamentation diameter (µm)

4-10

Mycorrhiza (ECM)

ECM Form

cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)

ECM color (young)

light yellow

ECM color (mature)

dark ochre or brownish

Cystidia form

long, sinuous, with thick walls and branched at various angles, septate with sometimes an elargment at the septa position

Cystidia color

ligth yellow to strong ochre

Cystidia Length (µm)

80-350

Cystidia Basal Diameter (µm)

2-3

Cystidia Position

apical, whole surface

Mantle Cells Form

epidermoid

Mantle cell density
(in a 20x20µm square)

5-10

Hosts

Host #1

Host Latin Name

Quercus cerris

Catalogue of Life Record

4R4KH

GBIF Record

2880580

Host #2

Host Latin Name

Quercus robur

Catalogue of Life Record

4R5YN

GBIF Record

2878688

Host #3

Host Latin Name

Corylus avellana

Catalogue of Life Record

YQ55

GBIF Record

2875979

Molecular Identification Methods

Method #9 - Marker: ITS

Forward Primer

(Tmacr For) CGTCGCTCATCAAAGCAGTC

Melting Temp (°C)

Reverse Primer

(Tmacr Rev) CCGCCAGTACCACCAGGAG

PCR Product Size (bps)

248

MgCl₂ Conc.

1.5 mM

Additives in mixture

PCR cycles

3 min at 94°C, 33 cycles: 94 °C 20 s, 61 °C 20 s,72 °C 20 s

Reference

Benucci GMN, Raggi L, Di Massimo G, Baciarelli‐Falini L, Bencivenga M, Falcinelli M, Albertini E (2011). Species‐specific primers for the identification of the ectomycorrhizal fungus Tuber macrosporum Vittad.. Molecular Ecology Resources, vol. 11, pp. 378-381. - doi: https://doi.org/10.1111/j.1755-0998.2010.02915.x

**Database for morphological and molecular identification of Tuber species**

Tuber macrosporum

Taxon

Ascocarp

Peridium

Gleba

Spores

Mycorrhiza (ECM)

Hosts

Host #1

Host #2

Host #3

Molecular Identification Methods

Method #9 - Marker: ITS

Contents