TuberID

Database for morphological and molecular identification of Tuber species

Tuber borchii

*
Ascocarp
*
Spores
*
Mycorrhizae (ECM)
*
Cystidia
*
Mantle

(Click on the above pictures to enlarge them)

Taxon

Species ID
16
Species Name
Tuber borchii
Clade
Puberulum
Index Fungorum Record
118774
MycoBank ID
123148
Fungal Names ID
118774
Catalogue of Life Record
59HXP
GBIF Record
5258463
Synonyms
-
Edible
Y
Authorship
Vittad.
Author Names
Vittadini C.
Reference
Monogr. Tuberac. (Milano): 44
First Description (Year)
1831

Ascocarp

Odour
Pleasant, sweetish
Ascocarp Form
tuberiform, lobed or knotty
Ascocarp Size (cm)
2-3 to 7

Peridium

External Morphology (Peridium)
Smooth or slightly pubescent
Peridium Color
dirty-white, ochre-brown to reddish brown
Wart Morphology
-
Wart Size (cm)
-

Gleba

Gleba Color at Maturity
whitish, beige to reddish brown
Gleba Aspect
marbled with whitish veins, wide and untidily arranged

Spores

Spore Form
ellipsoidal and subglobose
Spore Minimum Length (μm)
20
Spore Maximum Length (μm)
25
Spore Minimum Diameter (μm)
40
Spore Maximum Diameter (μm)
48
Spore color
brown-yellow to reddish brown
Spore ornamentation
reticulate-alveolate with very dense meshes almost regularly polygonal
Spore ornamentation height (µm)
-
Spore ornamentation diameter (µm)
5-6

Mycorrhiza (ECM)

ECM Form
cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)
ECM color (young)
yellow
ECM color (mature)
ochre-brown
Cystidia form
needle like, not ramified , sometimes with basal or median septa.
Cystidia color
colorless, hyaline
Cystidia Length (µm)
50-117
Cystidia Basal Diameter (µm)
2-4.5
Cystidia Position
whole surface
Mantle Cells Form
epidermoid
Mantle cell density
(in a 20x20µm square)
3-8

Hosts

Host #1
Host Latin Name
Quercus cerris
Catalogue of Life Record
4R4KH
GBIF Record
2880580
Host #2
Host Latin Name
Quercus robur
Catalogue of Life Record
4R5YN
GBIF Record
2878688
Host #3
Host Latin Name
Corylus avellana
Catalogue of Life Record
YQ55
GBIF Record
2875979
Host #5
Host Latin Name
Quercus pubescens
Catalogue of Life Record
4R5VW
GBIF Record
2881283
Host #6
Host Latin Name
Quercus suber
Catalogue of Life Record
4R678
GBIF Record
2879411
Host #7
Host Latin Name
Quercus ilex
Catalogue of Life Record
793PC
GBIF Record
2879098
Host #8
Host Latin Name
Carpinus betulus
Catalogue of Life Record
RG23
GBIF Record
2875818
Host #9
Host Latin Name
Ostrya carpinifolia
Catalogue of Life Record
6TCXD
GBIF Record
5332305
Host #12
Host Latin Name
Pinus nigra
Catalogue of Life Record
4J2D5
GBIF Record
5284809
Host #13
Host Latin Name
Pinus pinea
Catalogue of Life Record
77KSK
GBIF Record
5285165
Host #14
Host Latin Name
Pinus sylvestris
Catalogue of Life Record
4J2J5
GBIF Record
5285637
Host #15
Host Latin Name
Pinus halepensis
Catalogue of Life Record
4J27Y
GBIF Record
5285604
Host #23
Host Latin Name
Cistus incanus
Catalogue of Life Record
VLTH
GBIF Record
2874028
Host #24
Host Latin Name
Cistus salviifolius
Catalogue of Life Record
VLQL
GBIF Record
2874027
Host #25
Host Latin Name
Cistus albidus
Catalogue of Life Record
VLHX
GBIF Record
7331435

Molecular Identification Methods

Method #27 - Marker: ITS
Forward Primer
(TboI) TGTATGGGATGCCCTATCGGACT
Melting Temp (°C)
55
Reverse Primer
(TboII) CTATTACCACGGTCAACTTC
PCR Product Size (bps)
397
MgCl2 Conc.
1.5 mM
Additives in mixture
-
PCR cycles
94 °C 5 min, 30 cycles (94 °C 20 s, 55 °C for 15s, 72 °C for 30 s), final extension 72 °C 7 min.
Reference
Amicucci A, Zambonelli A, Giomaro G, Potenza L, Stocchi V (1998). Identification of ectomycorrhizal fungi of the genus Tuber by species-specific ITS primers. Molecular Ecology, vol. 7, pp. 273-277. - doi: https://www.researchgate.net/publication/230548556
Method #28 - Marker: ITS
Forward Primer
(TBA) TGCCCTATCGGACTCCCAAG
Melting Temp (°C)
65
Reverse Primer
(TBB) GCTCAGAACATGACTTGGAG
PCR Product Size (bps)
432
MgCl2 Conc.
-
Additives in mixture
-
PCR cycles
94 °C 5 min, 40 cycles (94 °C 45 s, 65 °C for 45s, 72 °C for 45 s), final extension 72 °C 5 min.
Reference
Mello, A., Garnero, L., & Bonfante, P. (1999). Specific PCR-primers as a reliable tool for the detection of white truffles in mycorrhizal roots. New Phytologist, vol. 141, pp. 511-516. - doi: https://doi.org/10.1046/j.1469-8137.1999.00356.x
Method #29 - Marker: ITS
Forward Primer
(rTboII) GAAGTTGACCGTGGTAATAG
Melting Temp (°C)
63
Reverse Primer
(ITS4) TCCTCCGCTTATTGATATGC
PCR Product Size (bps)
185
MgCl2 Conc.
6.7 mM
Additives in mixture
-
PCR cycles
94 °C 5 min, 30 cycles (94 °C 15 s, 63 °C for 15s, 72 °C for 30 s), final extension 72 °C 7 min.
Reference
Amicucci A, Guidi C, Zambonelli A, Potenza L, Stocchi V (2000). Multiplex PCR for the identification of white Tuber species. FEMS Microbiology Letters, vol. 189, pp. 265-269. - doi: https://doi.org/10.1111/j.1574-6968.2000.tb09241.x

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This project has received funding from European Union's Horizon 2020 research and innovation program under Grant Agreement #101007623.

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