TuberID

Database for morphological and molecular identification of Tuber species

Tuber puberulum

*
Ascocarp
*
Spores

(Click on the above pictures to enlarge them)

Taxon

Species ID
15
Species Name
Tuber puberulum
Clade
Puberulum
Index Fungorum Record
147428
MycoBank ID
28127
Fungal Names ID
147428
Catalogue of Life Record
59J36
GBIF Record
5258475
Synonyms
-
Edible
Y
Authorship
Berk. & Broome
Author Names
Berkeley M.J, Broome C.E.
Reference
Ann. Mag. nat. Hist., Ser. 1 18: 81
First Description (Year)
1846

Ascocarp

Odour
-
Ascocarp Form
globose, often with a basal cavity
Ascocarp Size (cm)
2-5

Peridium

External Morphology (Peridium)
smooth, pubescent
Peridium Color
grey-ochre to grey-hazel with dark spots
Wart Morphology
-
Wart Size (cm)
-

Gleba

Gleba Color at Maturity
yellow to brown-reddish
Gleba Aspect
marbled with withish veins irradiating from the base

Spores

Spore Form
spherical or subspherical
Spore Minimum Length (μm)
23
Spore Maximum Length (μm)
33
Spore Minimum Diameter (μm)
23
Spore Maximum Diameter (μm)
33
Spore color
yellow-brown
Spore ornamentation
reticulate-alveolate with almost regular poligonal meshes
Spore ornamentation height (µm)
-
Spore ornamentation diameter (µm)
3.5-7.5

Mycorrhiza (ECM)

ECM Form
cylindrical, staight, simple or ramified, monopodial-pyramidal (Angiosperm host). Dichotomous (Gymnosperm host)
ECM color (young)
whitish
ECM color (mature)
brown-yellow
Cystidia form
awl-shaped, bristle like, septated, not ramified
Cystidia color
colourless, hyaline
Cystidia Length (µm)
80-120
Cystidia Basal Diameter (µm)
3.8-4.2
Cystidia Position
whole surface
Mantle Cells Form
epidermoid
Mantle cell density
(in a 20x20µm square)
5-11

Hosts

Host #1
Host Latin Name
Quercus cerris
Catalogue of Life Record
4R4KH
GBIF Record
2880580
Host #2
Host Latin Name
Quercus robur
Catalogue of Life Record
4R5YN
GBIF Record
2878688
Host #3
Host Latin Name
Corylus avellana
Catalogue of Life Record
YQ55
GBIF Record
2875979
Host #5
Host Latin Name
Quercus pubescens
Catalogue of Life Record
4R5VW
GBIF Record
2881283
Host #6
Host Latin Name
Quercus suber
Catalogue of Life Record
4R678
GBIF Record
2879411
Host #7
Host Latin Name
Quercus ilex
Catalogue of Life Record
793PC
GBIF Record
2879098
Host #8
Host Latin Name
Carpinus betulus
Catalogue of Life Record
RG23
GBIF Record
2875818
Host #9
Host Latin Name
Ostrya carpinifolia
Catalogue of Life Record
6TCXD
GBIF Record
5332305
Host #12
Host Latin Name
Pinus nigra
Catalogue of Life Record
4J2D5
GBIF Record
5284809
Host #13
Host Latin Name
Pinus pinea
Catalogue of Life Record
77KSK
GBIF Record
5285165
Host #14
Host Latin Name
Pinus sylvestris
Catalogue of Life Record
4J2J5
GBIF Record
5285637
Host #15
Host Latin Name
Pinus halepensis
Catalogue of Life Record
4J27Y
GBIF Record
5285604
Host #23
Host Latin Name
Cistus incanus
Catalogue of Life Record
VLTH
GBIF Record
2874028
Host #24
Host Latin Name
Cistus salviifolius
Catalogue of Life Record
VLQL
GBIF Record
2874027
Host #25
Host Latin Name
Cistus albidus
Catalogue of Life Record
VLHX
GBIF Record
7331435

Molecular Identification Methods

Method #25 - Marker: ITS
Forward Primer
(TpuI) TCTGTTACCAGGGTCCACATT
Melting Temp (°C)
55
Reverse Primer
(TpuII) GGCTTCTGGGTTGAGGTGTTT
PCR Product Size (bps)
368
MgCl2 Conc.
1.5 mM
Additives in mixture
-
PCR cycles
94 °C 5 min, 30 cycles (94 °C 15 s, 55 °C for 30s), final extension 72 °C 7 min.
Reference
Amicucci A, Zambonelli A, Giomaro G, Potenza L, Stocchi V (1998). Identification of ectomycorrhizal fungi of the genus Tuber by species-specific ITS primers. Molecular Ecology, vol. 7, pp. 273-277. - doi: https://www.researchgate.net/publication/230548556
Method #26 - Marker: ITS
Forward Primer
(TpuI) TCTGTTACCAGGGTCCACATT
Melting Temp (°C)
55
Reverse Primer
(ITS4) TCCTCCGCTTATTGATATGC
PCR Product Size (bps)
560
MgCl2 Conc.
6.7 mM
Additives in mixture
-
PCR cycles
94 °C 5 min, 30 cycles (94 °C 15 s, 63 °C for 15s, 72 °C for 30 s), final extension 72 °C 7 min.
Reference
Amicucci A, Guidi C, Zambonelli A, Potenza L, Stocchi V (2000). Multiplex PCR for the identification of white Tuber species. FEMS Microbiology Letters, vol. 189, pp. 265-269. - doi: https://doi.org/10.1111/j.1574-6968.2000.tb09241.x

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This project has received funding from European Union's Horizon 2020 research and innovation program under Grant Agreement #101007623.

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Beatrice Belfiori, CNR-IBBR, Perugia (beatrice.belfiori@cnr.it)

Web Manager:

Gabriele Bucci, CNR-IBBR, Sesto Fiorentino, FI (gabriele.bucci@cnr.it)