TuberID

Database for morphological and molecular identification of Tuber species

Tuber rufum

*
Ascocarp
*
Spores

(Click on the above pictures to enlarge them)

Taxon

Species ID
14
Species Name
Tuber rufum
Clade
Rufum
Index Fungorum Record
250184
MycoBank ID
123143
Fungal Names ID
250184
Catalogue of Life Record
59J3D
GBIF Record
7900498
Synonyms
-
Edible
N
Authorship
Pollini
Author Names
Pollini
Reference
Giorn. Fis. Chim. Storia Nat. Med. Arti 9: 182
First Description (Year)
1816

Ascocarp

Odour
unpleasant
Ascocarp Form
subglobose to irregular
Ascocarp Size (cm)
1-3

Peridium

External Morphology (Peridium)
smooth to minutely verrucose
Peridium Color
reddish-ochre to brown-red
Wart Morphology
very small, flat, polygonal
Wart Size (cm)
-

Gleba

Gleba Color at Maturity
greyish, grey-pink to grey-brown
Gleba Aspect
firm, cartilagineous, with lighter veins, cottony white or translucid

Spores

Spore Form
ellipsoidal-roundish
Spore Minimum Length (μm)
16
Spore Maximum Length (μm)
24
Spore Minimum Diameter (μm)
24
Spore Maximum Diameter (μm)
30
Spore color
yellow-brown
Spore ornamentation
spines, regular, dense, sometimes slightly curved
Spore ornamentation height (µm)
2-3
Spore ornamentation diameter (µm)
-

Mycorrhiza (ECM)

ECM Form
cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)
ECM color (young)
brown-ochre
ECM color (mature)
dark-brown
Cystidia form
absent
Cystidia color
-
Cystidia Length (µm)
-
Cystidia Basal Diameter (µm)
-
Cystidia Position
-
Mantle Cells Form
epidermoid
Mantle cell density
(in a 20x20µm square)
5-10

Hosts

Host #1
Host Latin Name
Quercus cerris
Catalogue of Life Record
4R4KH
GBIF Record
2880580
Host #2
Host Latin Name
Quercus robur
Catalogue of Life Record
4R5YN
GBIF Record
2878688
Host #3
Host Latin Name
Corylus avellana
Catalogue of Life Record
YQ55
GBIF Record
2875979
Host #5
Host Latin Name
Quercus pubescens
Catalogue of Life Record
4R5VW
GBIF Record
2881283
Host #6
Host Latin Name
Quercus suber
Catalogue of Life Record
4R678
GBIF Record
2879411
Host #7
Host Latin Name
Quercus ilex
Catalogue of Life Record
793PC
GBIF Record
2879098
Host #8
Host Latin Name
Carpinus betulus
Catalogue of Life Record
RG23
GBIF Record
2875818
Host #9
Host Latin Name
Ostrya carpinifolia
Catalogue of Life Record
6TCXD
GBIF Record
5332305
Host #10
Host Latin Name
Tilia cordata
Catalogue of Life Record
56WPP
GBIF Record
3152047
Host #11
Host Latin Name
Tilia platyphyllos
Catalogue of Life Record
7CC2T
GBIF Record
3152048
Host #12
Host Latin Name
Pinus nigra
Catalogue of Life Record
4J2D5
GBIF Record
5284809
Host #13
Host Latin Name
Pinus pinea
Catalogue of Life Record
77KSK
GBIF Record
5285165
Host #14
Host Latin Name
Pinus sylvestris
Catalogue of Life Record
4J2J5
GBIF Record
5285637
Host #15
Host Latin Name
Pinus halepensis
Catalogue of Life Record
4J27Y
GBIF Record
5285604

Molecular Identification Methods

Method #23 - Marker: ITS
Forward Primer
(Ru1f) TGCTTTCCCAGGTGGTTGG
Melting Temp (°C)
63
Reverse Primer
(ITS4) TCCTCCGCTTATTGATATGC
PCR Product Size (bps)
566
MgCl2 Conc.
4 mM
Additives in mixture
-
PCR cycles
94 °C 3 min, 30 cycles (94 °C 20 s, 63 °C for 20s, 72 °C for 30 s), final extension 72 °C 10 min.
Reference
Iotti M, Amicucci A, Bonito G, Bonuso E, Stocchi V, Zambonelli A (2007). Selection of a set of specific primers for the identification of Tuber rufum: a truffle species with high genetic variability. . FEMS Microbiology Letters , vol. 277, pp. 223-231. - doi: https://doi.org/10.1111/j.1574-6968.2007.00963.x
Method #24 - Marker: ITS
Forward Primer
(Ru2f) TTGC TTTCCCAGGGAATTGG
Melting Temp (°C)
63
Reverse Primer
(ITS4) TCCTCCGCTTATTGATATGC
PCR Product Size (bps)
572
MgCl2 Conc.
4 mM
Additives in mixture
-
PCR cycles
94 °C 3 min, 30 cycles (94 °C 20 s, 63 °C for 20s, 72 °C for 30 s), final extension 72 °C 10 min.
Reference
Iotti M, Amicucci A, Bonito G, Bonuso E, Stocchi V, Zambonelli A (2007). Selection of a set of specific primers for the identification of Tuber rufum: a truffle species with high genetic variability. . FEMS Microbiology Letters , vol. 277, pp. 223-231. - doi: https://doi.org/10.1111/j.1574-6968.2007.00963.x

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This project has received funding from European Union's Horizon 2020 research and innovation program under Grant Agreement #101007623.

Contacts:

Andrea Rubini, CNR-IBBR, Perugia (andrea.rubini@cnr.it)
Claudia Riccioni, CNR-IBBR, Perugia (claudia.riccioni@cnr.it)
Beatrice Belfiori, CNR-IBBR, Perugia (beatrice.belfiori@cnr.it)

Web Manager:

Gabriele Bucci, CNR-IBBR, Sesto Fiorentino, FI (gabriele.bucci@cnr.it)