TuberID

Taxon

Species ID

Species Name

Tuber indicum

Clade

Melanosporum

Index Fungorum Record

188017

MycoBank ID

334557

Fungal Names ID

188017

Catalogue of Life Record

TW5LV

GBIF Record

5499365

Synonyms

Edible

Authorship

Cooke & Massee

Author Names

Cooke M.C, Massee G.E.

Reference

Grevillea 20 (no. 95): 67

First Description (Year)

1851

Ascocarp

Odour

pleasant

Ascocarp Form

globose to ellipsoidal

Ascocarp Size (cm)

2-3

Peridium

External Morphology (Peridium)

warty

Peridium Color

brown-black

Wart Morphology

irregularly polygonal, pentagonal or exagonal depressed, with splits along the sides

Wart Size (cm)

2-3

Gleba

Gleba Color at Maturity

purplish black

Gleba Aspect

marbled with numerous, very thin, whitish veins.

Spores

Spore Form

ellipsoidal, occasionally subglobose

Spore Minimum Length (μm)

Spore Maximum Length (μm)

Spore Minimum Diameter (μm)

Spore Maximum Diameter (μm)

Spore color

pale brown to dark brown

Spore ornamentation

spines wide at the base, blunt and slightly hooked at the apices, free, broader than T. melanosporum

Spore ornamentation height (µm)

3-5

Spore ornamentation diameter (µm)

Mycorrhiza (ECM)

ECM Form

cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)

ECM color (young)

pale-yellow

ECM color (mature)

brown-dark brown

Cystidia form

Simple or branched, smooth, septate, slightly tortuous, pale brown, some with right angle ramification

Cystidia color

pale brown

Cystidia Length (µm)

up to 390

Cystidia Basal Diameter (µm)

1.7–3.4

Cystidia Position

Mantle Cells Form

epidermoid

Mantle cell density
(in a 20x20µm square)

3-5

Hosts

Host #26

Host Latin Name

Pinus armandii

Catalogue of Life Record

4J22Q

GBIF Record

5285203

Host #27

Host Latin Name

Castanea mollissima

Catalogue of Life Record

RNTN

GBIF Record

5333237

Molecular Identification Methods

Method #21 - Marker: ITS

Forward Primer

(ITSCHCH) AACAACAGACTTTGTAAAGGGTTG

Melting Temp (°C)

Reverse Primer

(ITS4LNG) TGATATGCTTAAGTTCAGCGGG

PCR Product Size (bps)

140

MgCl₂ Conc.

4 mM

Additives in mixture

PCR cycles

95 °C 3 min, 23 cycles (94 °C 30 s, 63 °C for 30 s, 72 °C for 45 s), final extension 72 °C 7 min.

Reference

Paolocci F, Rubini A, Granetti B, Arcioni S (1999). Rapid molecular approach for a reliable identification of Tuber spp. ectomycorrhizae. . FEMS Microbiology Ecology, vol. 28, pp. 23-30. - doi: https://doi.org/10.1111/j.1574-6941.1999.tb00557.x

Method #22 - Marker: ITS

Forward Primer

(Indi-fw) AACAACAGACTTTGTAAAGGGTT

Melting Temp (°C)

Reverse Primer

(ITS4LNG) TGATATGCTTAAGTTCAGCGGG

PCR Product Size (bps)

146

MgCl₂ Conc.

Additives in mixture

PCR cycles

95 °C 5 min, 30 cycles (95 °C 20 s, 60 °C for 45 s), final extension 72 °C 10 min.

Reference

Schelm S, Siemt M, Pfeiffer J, Lang C, Tichy H-V, Fischer M (2020). Food authentication: identification and quantitation of different Tuber species via capillary gel electrophoresis and real-time PCR.. Food , vol. 9, pp. 501. - doi: https://doi.org/10.3390/foods9040501

**Database for morphological and molecular identification of Tuber species**

Tuber indicum

Taxon

Ascocarp

Peridium

Gleba

Spores

Mycorrhiza (ECM)

Hosts

Host #26

Host #27

Molecular Identification Methods

Method #21 - Marker: ITS

Method #22 - Marker: ITS

Contents