TuberID

Database for morphological and molecular identification of Tuber species

Tuber brumale

*
Ascocarp
*
Spores

(Click on the above pictures to enlarge them)

Taxon

Species ID
10
Species Name
Tuber brumale
Clade
Melanosporum
Index Fungorum Record
228019
MycoBank ID
241947
Fungal Names ID
228019
Catalogue of Life Record
59HXS
GBIF Record
5258455
Synonyms
Tuber moschatum, Tuber brumale var. moschatum
Edible
Y
Authorship
Vittad.
Author Names
Vittadini C.
Reference
Monogr. Tuberac. (Milano): 44
First Description (Year)
1831

Ascocarp

Odour
strong, variable, pleasant, musky
Ascocarp Form
subglobose to irregular
Ascocarp Size (cm)
0.6-4

Peridium

External Morphology (Peridium)
warty
Peridium Color
greyish to black
Wart Morphology
penta- or hexagonal flat warts, easily detachable
Wart Size (cm)
1.4-2.4

Gleba

Gleba Color at Maturity
greyish, pale sepia
Gleba Aspect
marbled, with few broad white veins

Spores

Spore Form
ellipsoidal
Spore Minimum Length (μm)
16
Spore Maximum Length (μm)
19
Spore Minimum Diameter (μm)
25
Spore Maximum Diameter (μm)
33
Spore color
yellowish-brown
Spore ornamentation
spines, needle like, rigid and sharp
Spore ornamentation height (µm)
3–7
Spore ornamentation diameter (µm)
-

Mycorrhiza (ECM)

ECM Form
cylindrical or club shaped with rounded end. Simple or ramified. Monopodial pinnate or monopodial piramidal (Angiosperm host). Dichotomous (Gymnosperm host)
ECM color (young)
ochre
ECM color (mature)
brown
Cystidia form
needle like, not ramified, with enlarged base and sometimes with a basal septum
Cystidia color
yellow
Cystidia Length (µm)
20-155
Cystidia Basal Diameter (µm)
3-6
Cystidia Position
prevalently apical
Mantle Cells Form
epidermoid
Mantle cell density
(in a 20x20µm square)
4-8

Hosts

Host #1
Host Latin Name
Quercus cerris
Catalogue of Life Record
4R4KH
GBIF Record
2880580
Host #2
Host Latin Name
Quercus robur
Catalogue of Life Record
4R5YN
GBIF Record
2878688
Host #3
Host Latin Name
Corylus avellana
Catalogue of Life Record
YQ55
GBIF Record
2875979
Host #4
Host Latin Name
Fagus sylvatica
Catalogue of Life Record
3DSK5
GBIF Record
2882316
Host #5
Host Latin Name
Quercus pubescens
Catalogue of Life Record
4R5VW
GBIF Record
2881283
Host #9
Host Latin Name
Ostrya carpinifolia
Catalogue of Life Record
6TCXD
GBIF Record
5332305
Host #10
Host Latin Name
Tilia cordata
Catalogue of Life Record
56WPP
GBIF Record
3152047
Host #11
Host Latin Name
Tilia platyphyllos
Catalogue of Life Record
7CC2T
GBIF Record
3152048
Host #12
Host Latin Name
Pinus nigra
Catalogue of Life Record
4J2D5
GBIF Record
5284809
Host #13
Host Latin Name
Pinus pinea
Catalogue of Life Record
77KSK
GBIF Record
5285165
Host #14
Host Latin Name
Pinus sylvestris
Catalogue of Life Record
4J2J5
GBIF Record
5285637
Host #15
Host Latin Name
Pinus halepensis
Catalogue of Life Record
4J27Y
GBIF Record
5285604

Molecular Identification Methods

Method #20 - Marker: ITS
Forward Primer
(ITSB) CAATGTCAGAGCCAATCTAATGC
Melting Temp (°C)
63
Reverse Primer
(ITS4LNG) TGATATGCTTAAGTTCAGCGGG
PCR Product Size (bps)
700
MgCl2 Conc.
4 mM
Additives in mixture
-
PCR cycles
95 °C 3 min, 23 cycles (94 °C 30 s, 63 °C for 30 s, 72 °C for 45 s), final extension 72 °C 7 min.
Reference
Paolocci F, Rubini A, Granetti B, Arcioni S (1999). Rapid molecular approach for a reliable identification of Tuber spp. ectomycorrhizae. . FEMS Microbiology Ecology, vol. 28, pp. 23-30. - doi: https://doi.org/10.1111/j.1574-6941.1999.tb00557.x

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All the material on these pages is released under the license Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

This project has received funding from European Union's Horizon 2020 research and innovation program under Grant Agreement #101007623.

Contacts:

Andrea Rubini, CNR-IBBR, Perugia (andrea.rubini@cnr.it)
Claudia Riccioni, CNR-IBBR, Perugia (claudia.riccioni@cnr.it)
Beatrice Belfiori, CNR-IBBR, Perugia (beatrice.belfiori@cnr.it)

Web Manager:

Gabriele Bucci, CNR-IBBR, Sesto Fiorentino, FI (gabriele.bucci@cnr.it)