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Institute of Biosciences and Bioresources

National Research Council of Italy

Maurizio Tamburrini

Role: Senior Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132712
E-mail: maurizio.tamburrini@ibbr.cnr.it


Curriculum Vitae

Date of birth: October 7, 1958

1982. Degree in Biological Sciences.

1983. Awarded a fellowship of the National Research Council of Italy (CNR).

1983 to 1987. Awarded a fellowship for a Research Doctorate Course in Biochemical Sciences, Universities of Naples and Bari, Italy.

1984 to date. Member of the Italian National Biologists Order.

1985 to date. Member of the Italian Society of Biochemistry and Molecular Biology.

1988. Research Doctor in Biochemical Sciences.

1988. Post-doctoral position at the Roche Institute of Molecular Biology, Nutley, New Jersey, USA.

1989 to 1997. Researcher at the CNR Institute of Protein Biochemistry and Enzymology, Naples.

1990-1991 and 1993-1994. Member of the Sixth and of the Ninth Italian Antarctic Expeditions, Terra Nova Bay Scientific Station, Antarctica.

1990 and 1993. Guest Scientist at the New Zealand Scientific Antarctic Station “Scott Base”.

1991. Visiting Investigator at the Leigh Marine Laboratory, University of Auckland, New Zealand.

1992. Member of the Antarctic Expedition ANT X/3, Weddell Sea, Antarctica, on board R/V “Polarstern”, Alfred Wegener Institut, Bremerhaven, Germany.

1997 to 2013. Senior Researcher at the CNR Institute of Protein Biochemistry, Naples.

2014 to date. Senior Researcher at the CNR Institute of Biosciences and BioResources, UOS Naples.

Research interests

Study of the structural and functional properties of food and environmental proteins and peptides, and of their physiological role and impact on human health. Protein purification from the natural sources and identification. Protein characterisation, including elucidation of the primary structure and of post-translational modifications by amino acid sequencing and mass spectrometry, and study of the secondary and tertiary structure by circular dichroism spectroscopy and bioinformatic tools. Functional characterisation, including kinetic studies of enzymatic activities.

Current research topics

  • Identification and purification of new allergenic proteins from their natural food or environmental sources.
  • Structural and immunological characterisation of the molecules under investigation.
  • Analysis of the protein and allergen profile in different foods.
  • Stability of specific allergens under different environmental/experimental conditions, including heat stability and gastrointestinal digestion.
  • Influence of several factors (ripening stage, post-harvest treatments, cultivar, etc) on the variation of the protein and allergen profiles of plant foods.
  • Study of nutraceutical peptides from plant food. Currently our attention is focused on kissper, a peptide isolated from green kiwifruit and showing ion channeling activity in sinthetic membranes, as well as antinflammatory effect on human intestinal cells from patients suffering from Crohn’s disease.

Investigation of the protein biological activities, of their possible effects on human health and of their biomedical application involves collaboration with research groups having appropriate expertise in the fields of 3D-structure determination, physiology and clinics.

Current main collaborations

Prof. Adriano Mari, Associated Centers for Molecular Allergology (CAAM), Rome

(www.caam-allergy.com/)

Prof. Maksymilian Chruszcz, Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, USA

(www.chem.sc.edu/)

Prof. Delia Picone, Department of Chemical Sciences, University of Naples Federico II, Napoli

(www.docenti.unina.it/delia.picone)

Dr Silvia Pasquariello, C.R.A. Research Unit on Fruit Trees, Caserta

(http://sito.entecra.it/portale/cra_dati_istituto.php?id=233&lingua=EN)

Prof. Carolina Ciacci, Gastrointestinal Unit, Department of Medicine and Surgery, University of Salerno

(www.unisa.it/dipartimenti/dipmedchi/index)

Related publications

Tuppo L., Spadaccini R., Alessandri C., Wienk H., Boelens R., Giangrieco I., Tamburrini M., Mari A., Picone D., Ciardiello M.A. (2014) Structure, stability, and IgE binding of the peach allergen Peamaclein (Pru p 7). Biopolymers: Peptide Science 102(5):416-425, doi: 10.1002/bip.22530.

Ciacci C., Russo I., Bucci C., Iovino P., Pellegrini L., Giangrieco I., Tamburrini M., Ciardiello M.A. (2014) The Kiwifruit Peptide Kissper Displays Anti-Inflammatory and Antioxidant Effects in In Vitro And Ex Vivo Human Intestinal Models. Clin. Exp. Immunol. 175(3):476-484.

Ciardiello M.A., Tamburrini M., Liso M., Crescenzo R., Rafaiani C., Mari A. (2013) Food allergen profiling: A big challenge. Food Research International 54:1033-1041.

Tuppo L., Alessandri C., Pomponi D., Picone D., Tamburrini M., Ferrara R., Petriccione M., Mangone I., Palazzo P., Liso M., Giangrieco I., Crescenzo R., Bernardi M.L., Zennaro D., Helmer-Citterich M., Mari A., Ciardiello M.A. (2013) Peamaclein - a new peach allergenic protein: similarities, differences and misleading features compared to Pru p 3. Clin. Exp. Allergy 43:128-140.

Palazzo P., Tuppo L., Giangrieco I., Bernardi M.L., Rafaiani C., Crescenzo R., Tamburrini M., Zuzzi S., Alessandri C., Mari A., Ciardiello M.A. (2013) Prevalence and peculiarities of IgE reactivity to kiwifruit pectin methylesterase and its inhibitor, Act d 7 and Act d 6, in subjects allergic to kiwifruit. Food Research International 53:24-30.

Pasquariello M.S., Palazzo P., Tuppo L., Liso M., Petriccione M., Rega P., Tartaglia A., Tamburrini M., Alessandri C., Ciardiello M.A., Mari A. (2012) Analysis of the potential allergenicity of traditional apple cultivars by Multiplex Biochip-Based Immunoassay. Food Chemistry 135(1):219-227.

Giangrieco I., Rafaiani C., Liso M., Palazzo P., Pomponi D., Tuppo L., Crescenzo R., Tamburrini M., Mari A., Ciardiello M.A. (2012) Allergens in allergy diagnosis: a glimpse at emerging new concepts and methodologies. Translational Medicine @ UniSa 4:27-33.

Bernardi M.L., Giangrieco I., Camardella L., Ferrara R., Palazzo P., Panico M.R., Crescenzo R., Carratore V., Zennaro D., Liso M., Santoro M., Zuzzi S., Tamburrini M., Ciardiello M.A., Mari A. (2011) Allergenic lipid transfer proteins from plant-derived foods do not immunologically and clinically behave homogeneously: the kiwifruit LTP as a model. PLoS One 6(11):e27856.

Mari A., Ciardiello M.A., Tamburrini M., Rasi C., Palazzo P. (2010) Proteomic analysis in the identification of allergenic molecules. Expert Rev. Proteomics 7(5):723-734.

Bernardi M.L., Picone D., Tuppo L., Giangrieco I., Petrella G., Palazzo P., Ferrara R., Tamburrini M., Mari A., Ciardiello M.A. (2010) Physico-chemical features of the environment affect the protein conformation and the immunoglobulin E reactivity of kiwellin (Act d 5). Clin. Exp. Allergy 40:1819-1826.

Ciardiello M.A., Palazzo P., Bernardi M.L., Carratore V., Giangrieco I., Longo V., Melis M., Tamburrini M., Zennaro D., Mari A., Colombo P. (2010) Biochemical, immunological and clinical characterization of a crossreactive nonspecific lipid transfer protein 1 from mulberry. Allergy 65(5):597-605.

Ciardiello M.A., Giangrieco I., Tuppo L., Tamburrini M., Buccheri M., Palazzo P., Bernardi M.L., Ferrara R., Mari A. (2009) Influence of the natural ripening stage, cold storage, and ethylene treatment on the protein and IgE-binding profiles of green and gold kiwi fruit extracts. J. Agric. Food Chem. 57(4):1565-1571.

Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V., Tamburrini M., Mari A., Ciardiello M.A. (2008) Kiwellin, a modular protein from green and gold kiwi fruits: evidence of in vivo and in vitro processing and IgE binding. J. Agric. Food Chem. 56:3812-3817.

Ciardiello M.A., Meleleo D., Saviano G., Crescenzo R., Carratore V., Camardella L., Gallucci E., Micelli S., Tancredi T., Picone D., Tamburrini M. (2008) Kissper, a kiwi fruit peptide with channel-like activity: structural and functional features. J. Pept. Sci. 14(6):742-754.

Ciardiello M.A., D’avino R., Amoresano A., Tuppo L., Carpentieri A., Carratore V., Tamburrini M., Giovane A., Pucci P., Camardella L. (2008) The peculiar structural features of kiwi fruit pectin methylesterase: Amino acid sequence, oligosaccharides structure, and modeling of the interaction with its natural proteinaceous inhibitor. Proteins 71(1):195-206.

Buccheri M., Carratore V., Camardella L., Tamburrini M., Ciardiello M.A. (2006) Valutazione della biodiversità in alcuni fruttiferi mediterranei mediante analisi del proteoma. Italus Hortus 13 (2):190-193.

Tamburrini M., Cerasuolo I., Carratore V., Stanziola A.A., Zofra S., Romano L., Camardella L., Ciardiello M.A. (2005) Kiwellin, a novel protein from kiwi fruit. Purification, biochemical characterization and identification as an allergen. Protein J. 24:423-429.

Ciardiello M.A., Tamburrini M., Tuppo L., Carratore V., Giovane A., Mattei B., Camardella L. (2004) Pectin methylesterase from kiwi and kaki fruits: purification, characterization and role of pH in the enzyme regulation and interaction with the kiwi proteinaceous inhibitor. J. Agric. Food Chem. 52:7700-7703.

Giovane A., Servillo L., Balestrieri C., Raiola A., D’Avino R., Tamburrini M., Ciardiello M.A., Camardella L. (2004) Pectin methylesterase inhibitor. Biochim. Biophys. Acta 1696(2):245-252.

Scognamiglio M.A., Ciardiello M.A., Tamburrini M., Carratore V., Rausch T., Camardella L. (2003) The plant invertase inhibitor shares structural properties and disulfide bridges arrangement with the pectin methylesterase inhibitor. J. Prot. Chem. 22(3):363-369.

 

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