In vitro propagation of ash (Fraxinus spp.) by somatic embryogenesis
Capuana M
In: "Protocols for Micropropagation of Selected Economically-Important Horticultural Plants" (Lambardi M, Ozudogru EA, Jain SM eds). Humana Press-Springer, Dordrecht, pp. 213-222. ISBN 978-1-62703-073-1) (2012)
Induction of somatic embryogenesis is described in common ash (Fraxinus excelsior L.). Embryogenictissues are obtained from immature zygotic embryos and cultured on a modified Murashige and Skoogmedium containing 8.8 mM 2.4-dichlorophenoxyacetic acid and 4.4 mM benzyl-adenine. Embryogenictissue is subcultured and multiplied on medium supplemented with reduced concentration of plantgrowth hormones. Somatic embryos develop and mature by transfer to hormone-free medium and subsequentculture on medium containing low amount of benzyladenine. Somatic embryo germination andconversion are enhanced by cold storage at 4°C and successive transfer onto Woody Plant Medium(WPM). Fully developed plantlets are then transferred to pots and acclimatized in the greenhouseequipped with a mist system.
