Wilma Sabetta

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Permanent Researcher at CNR IBBR Bari, Italy, since July 2019

Professional experiences and training

2019-June 2019 - Post-Doc research contract on the project INNONETWORK DOMINA APULIAE "Women, wine, age: Apulian native grapes with high antioxidant content for a healthier aging". Transcriptomic analysis of the most promising Apulian grapes with high antioxidant content and identification of genes involved in polyphenols metabolic pathway. University of Bari, Department of Biosciences, Biotechnologies and Biopharmaceuticals (Di.B.B.B.). Area: Plant genetics and breeding.

2017-2018 - Contract as freelance doctor for the "Italian Variety Club" Network: Coordination of genetic analysis (Marker Assisted Selection) and data processing in the framework for seedless degree evaluation in new grape cultivars. University of Bari Aldo Moro, Department of Soil, Plant and Food Sciences (Di.S.S.P.A.). Area: Plant genetics and breeding.

2017 - Qualification license as biologist at University of Bari "Aldo Moro".

2016-2017 - Coordinated and continued collaboration on the CLUSTER project Support to Regional Technological Clusters: "Innovation of processes and products in the sector of sparkling wines from native Apulian grape (IProViSP)". Molecular characterization of autochthonous grapes, transcriptomic analysis of the best varieties, development of a Knowledge Portal and result divulgation. SINAGRI University Spin Off, Bari. Area: Plant genetics and breeding.

2015 - Coordinated and continued collaboration for the development of suitable protocols for plant food tracking and tracing by means of molecular markers (SSR). SINAGRI University Spin Off, Bari. Area: Plant genetics and breeding.

2015 - Coordinated and continued collaboration on the multidisciplinary project Re.Ge.Fru.P: Genetic analysis of the Apulian fruit germplasm and molecular data processing. SINAGRI University Spin Off, Bari. Area: Plant genetics and breeding.

2012-2015 - Post-Doc research contract on the FIRB project "Study of cyclic nucleotide role in plant defence responses to biotic stresses". Genetic and proteomic analysis of model species, such as Arabidopsis thaliana and tobacco cellular line Bright Yellow-2 (TBY-2), wild-type and transgenic, in basal and stress conditions (biotic and abiotic); manipulation, cloning and expression of genes of interest; expression and purification of exogenous proteins; bioinformatic analysis of differentially expressed genes and transcription factors. Institute of Biosciences and BioResources (IBBR), Bari, and Max Planck Institute, Golm-Potsdam (Berlin, Germany). Area: Plant Genomics, Proteomics and Physiology.

2009-2012 - Post-Doc research contract on the PS59 Strategic Project: EcoTILLING Analysis of olive collections; molecular characterization of plant material by SNP, SSR, RAPD, AFLP and TRAP markers; tracking and tracing of olive and cereal food chains by molecular technologies; in vitro culture of olive immature embryos and plant explants; map populations and molecular/phenotypic analysis; genetic transformation of wheat immature embryos by Agrobacterium tumefaciens and plant regeneration; management of field activities. University of Bari Aldo Moro, Department of Soil, Plant and Food Sciences (Di.S.S.P.A.). Area: Plant Genetics and Breeding, Functional Genomics.

2005-2009 - PhD in Molecular Breeding and Pathology of Crops and Forest Species, thesis entitled: "Detection of EMS-induced point mutations by TILLING technology in barley and sunflower genes involved in BaYMV Complex resistance and oil quality components". Development of Helianthus annuus TILLING platform; identification, manipulation and characterization of sunflower and barley genes; screening of Hordeum vulgare TILLING collection. University of Bari, Department of Soil, Plant and Food Sciences (DISSPA) and Leibnitz Institute of Plant Genetics and Crop Plant Research (IPK) (Gatersleben, Germany). Area: Plant Genetics and Breeding, Functional Genomics.

2005 - Project collaboration: Protein in vitro expression by the RTS 500 (Rapid Translation System) and protein analysis by SDS-PAGE, affinity-, gel filtration- and anion exchange-chromatography. University of Salento, Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.). Area: Plant Physiology.

2004 - Graduation in Crop Plant Biotechnology, thesis entitled: "Molecular characterization of yeast antagonists of post-harvest pome fruit pathogens by AFLP markers". University of Turin, Department of Valorization and Protection of Agroforestry Resources (Di.Va.P.R.A.). Area: Plant Pathology, Biotechnology and Genetics.

2003-2004 - Project collaboration: Conservation of microbial isolates and their classification by RFLP-PCR on ribosomal DNA. University of Turin, Department of Valorization and Protection of Agroforestry Resources (Di.Va.P.R.A.). Area: Plant Pathology and Biotechnology.

Main research topics

• Molecular characterization, valorisation and use of plant genetic resources
• Molecular breeding of crop species
• Gene isolation and functional characterization
• Genetic engineering of crop and model species
• Genomics, transcriptomics and proteomics
• Tracking and tracing in food chains
• Reverse genetics (TILLING and EcoTILLING)
• Main species of interest: olive, grape, sunflower, wheat

Other activities

• Member of SINAGRI University Spin-off s.r.l. - "Advanced services for sustainability and innovation in agricultural and rural areas". LTD, vat number: 07331290721; sinagri.it
• Active attendee in several seminars, refresher courses and programmatic meetings in national and international research institutes
• Speaker at several national and international conferences
• Reviewer of numerous scientific publications and proposals
• Supervisor of several students for Master and Specialist Degrees at University of Bari "Aldo Moro"
• Organization and management of teamwork, laboratory needs and field activities.

Responsibility in Research Projects

• Title: INOGRAPE - Evaluation of possible genetic sources potentially useful for table grape breeding (JointProject 2018)

Role: Partner supervisor for the identification of genes and regulatory factors involved in grape seed development and for the identification of new genetic resources useful for seedless table grapes.

Duration: 3 years.

Lista Pubblicazioni

(lista completa disponibile su CNR People)

Biodiversity Evaluation and Preservation of Italian Stone Fruit Germplasm (Peach and Apricot) in Southern Italy

Savoia MA, Del Faro L, Turco A, Fanelli V, Venerito P, Montemurro C, Sabetta W

Anno: 2023

ll Fascino Sostenibile delle Piante: dal mondo della ricerca all’Agenda 2030

Cillo F, Stavolone L, Carluccio AV, Fanelli E, De Luca F, Troccoli A, Sabetta W, Mangini G, De Paola D, De Tullio M

Anno: 2022

“Good Wine Makes Good Blood”: An Integrated Approach to Characterize Autochthonous Apulian Grapevines as Promising Candidates for Healthy Wines

Sabetta W, Centrone M, D’agostino M, Difonzo G, Mansi L, Tricarico G, Venerito P, Picardi E, Ceci LR, Tamma G, Caponio F, Montemurro C, Volpicella M

Anno: 2022

Mysterious travellers: Identification of viral and host defense-related components in tomato fruit-derived nanovesicles

Sabetta W, Mammadova R, Kralj-iglic V, Schlosper G, Pocsfalvi G, Cillo F

Anno: 2022

The Relevance of Discovering and Recovering the Biodiversity of Apulian Almond Germplasm by Means of Molecular and Phenotypic Markers

Savoia MA, Del Faro L, Venerito P, Gaeta L, Palasciano M, Montemurro C, Sabetta W

Anno: 2022

Functional conservation of the grapevine candidate gene INNER NO OUTER for ovule development and seed formation

di Rienzo V, Imanifard Z, Mascio I, Gasser CS, Skinner DJ, Pierri CL, Marini M, Fanelli V, Sabetta W, Montemurro C, Bellin D

Anno: 2021

A TILLING by sequencing approach to identify induced mutations in sunflower genes

Fanelli V, Ngo J K, Thompson L V, Silva R B, Tsai H, Sabetta W, Montemurro C, Comai L, Harmer L S

Anno: 2021

Bioactive Potential of Minor Italian Olive Genotypes from Apulia, Sardinia and Abruzzo

Sabetta W, Mascio I, Squeo G, Gadaleta S, Flamminii F, Conte P, Di Mattia CD, Piga A, Caponio F, Montemurro C

Anno: 2021

Serendipitous in situ conservation of Faba bean landraces in Tunisia: a case study

Babay E, Khamassi K, Sabetta W, Miazzi MM, Montemurro C, Pignone D, Danzi D, Finetti-Sialer MM, Mangini G

Anno: 2020

Re.Ger.O.P.: An Integrated Project for the Recovery of Ancient and Rare Olive Germplasm

Miazzi MM, di Rienzo V, Mascio I, Montemurro C, Sion S, Sabetta W, Vivaldi GA, Camposeo S, Caponio F, Squeo G, Difonzo G, Loconsole G, Bottalico G, Venerito P, Montilon V, Saponari A, Altamura G, Mita G, Petrontino A, Fucilli V, Bozzo F

Anno: 2020

A simple and rapid method for genomic DNA extraction and microsatellite analysis in tree plants

Alice S, Sara S, Susanna G, Antonio SM, Luciana P, Valentina F, Valentina DR, Francesca T, Marilena MM, Cinzia M, Wilma S

Anno: 2019

Valorization of autochthonous Apulian grapevine varieties for spumante production

Fanelli V, Volpicella M A, Giampetruzzi A, Saldarelli P, Leoni C -, Ceci LR, Di Rienzo V, Venerito P, Taranto F, Giannini P, Bozzo F, Montemurro C -, Sabetta W A

Anno: 2019

Genotyping-by-sequencing-derived single-nucleotide polymorphism catalog from a grapevine ( Vitis vinifera L.) germplasm collection that includes the most representative Apulian autochthonous cultivars

Miazzi M, D’agostino N, Gadaleta S, Di Rienzo V, Fanelli V, Sabetta W, Montemurro C, Taranto F

Anno: 2019

Self-Incompatibility Assessment of Some Italian Olive Genotypes (Olea europaea L.) and Cross-Derived Seedling Selection by SSR Markers on Seed Endosperms

Montemurro C, Dambruoso G, Bottalico G, Sabetta W

Anno: 2019

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

Sabetta W, Vandelle E, Locato V, Costa A, Cimini S, Bittencourt Moura A, Luoni L, Graf A, Viggiano L, De Gara L, Bellin D, Blanco E, Pinto MC

Anno: 2019

GBS-derived SNP catalogue unveiled wide genetic variability and geographical relationships of Italian olive cultivars

D’agostino N, Taranto F, Camposeo S, Mangini G, Fanelli V, Gadaleta S, Miazzi MM, Pavan S, di Rienzo V, Sabetta W, Lombardo L, Zelasco S, Perri E, Lotti C, Ciani E, Montemurro C

Anno: 2018

Genetic flow among olive populations within the Mediterranean basin

di Rienzo V, Sion S, Taranto F, D’agostino N, Montemurro C, Fanelli V, Sabetta W, Boucheffa S, Tamendjari A, Pasqualone A, Zammit-Mangion M, Miazzi MM

Anno: 2018

Single nucleotide polymorphism (SNP) diversity in an olive germplasm collection

Taranto F, A, ND’Agostino, Pavan S, Fanelli V, V Di Rienzo, Sabetta W, Miazzi MM, Zelasco S, Perri E, Montemurro C

Anno: 2018

The preservation and characterization of Apulian olive germplasm biodiversity

V Di Rienzo, A, Miazzi MM, Fanelli V, Sabetta W, Montemurro C

Anno: 2018

Chemistry, biosynthesis, and antioxidative function of glutathione in plants

Sabetta W, Paradiso A, Paciolla C, de Pinto MC

Anno: 2017

A reliable analytical procedure to discover table grape DNA adulteration in industrial wines and musts

V Di Rienzo, Fanelli V, Miazzi MM, --Savino V, Pasqualone A, Summo C, Giannini P, Sabetta W, -Montemurro C

Anno: 2017

Nitric oxide and reactive oxygen species in PCD signaling

Locato V, Paradiso A, Sabetta W, De Gara L, de Pinto MC

Anno: 2016

Chemical and molecular characterization of crude oil obtained by olive-pomace recentrifugation

Pasqualone A, Di Rienzo V, Sabetta W, Fanelli V, Summo C, Paradiso VM, Montemurro C, Caponio F

Anno: 2016

Screening auxin response, in vitro culture aptitude and susceptibility to Agrobacterium-mediated transformation of Italian commercial durum wheat varieties

Sabetta W, Crosatti C, Soltész A, Di Rienzo V, Montemurro C

Anno: 2016

Traceability of PDO olive oil “Terra di Bari” using high resolution melting

Montemurro C, Miazzi MM, Pasqualone A, Fanelli V, Sabetta W, di Rienzo V

Anno: 2015

Fad7 gene identification and fatty acids phenotypic variation in an olive collection by EcoTILLING and sequencing approaches

Sabetta W, Blanco A, Zelasco S, Lombardo L, Perri E, Mangini G, Montemurro C

Anno: 2013

sunTILL: a TILLING resource for gene function analysis in sunflower

Sabetta W, Alba V, Blanco A, Montemurro C

Anno: 2011

Similarity Patterns and Stability of Environmental Response in Sunflower Hybrids

Alba V, Polignano GB, Montemurro C, Sabetta W, Bisignano V, Turi M, Ravaglia S, Troccoli A, Colecchia SA, Alba E, Blanco A

Anno: 2010

Essential oils, genetic relationships and in vitro establishment of Helichrysum italicum (Roth) G. Don ssp. italicum from wild Mediterranean germplasm

Morone-Fortunato I, Montemurro C, Ruta C, Perrini R, Sabetta W, Blanco A, Lorusso E, Avato P

Anno: 2010

SSR-based identification key of cultivars of Olea europaea L. diffused in Southern-Italy

Alba V, Montemurro C, Sabetta W, Pasqualone A, Blanco A

Anno: 2009

Microsatellite markers to identify specific alleles in DNA extracted from monovarietal virgin olive oils

Alba V, Sabetta W, Blanco A, Pasqualone A, Montemurro C

Anno: 2009

DNA microsatellite region for a reliable quantification of soft wheat adulteration in durum wheat-based foodstuffs by real-time PCR

Sonnante G, Montemurro C, Morgese A, Sabetta W, Blanco A, Pasqualone A

Anno: 2009

AFLP molecular markers to identify virgin olive oils from single Italian cultivars

Montemurro C, Pasqualone A, Simeone R, Sabetta W, Blanco A

Anno: 2008

Use of AFLP for differentiation of Metschnikowia pulcherrima strains for postharvest disease biological control

Spadaro D, Sabetta W, Acquadro A, Portis E, Garibaldi A, Gullino M

Anno: 2008

Characterization of the main Syrian olive cultivars

Khatib M, Achtar S, Blanco A, Sabetta W, Montemurro C

Anno: 2007

Effectiveness of microsatellite DNA markers in checking the identity of protected designation of origin extra virgin olive oil

Pasqualone A, Montemurro C, Summo C, Sabetta W, Caponio F, Blanco A

Anno: 2007