*

Institute of Biosciences and Bioresources

National Research Council of Italy

Stefania Filosa

Role: Senior Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132717
E-mail: stefania.filosa@ibbr.cnr.it


Research interest

Stefania Filosa gruop activity is mainly focused on understanding the role of oxidative stress in different biological processes such as apoptosis and differentiation.

Oxidative stress is linked to pathological and physiological processes including aging. NADPH is critical in maintaining appropriate levels of the cellular redox buffer GSH. NADPH is mainly generated by Glucose-6-phosphate dehydrogenase (G6PD), the first and key Pentose Phosphate Pathway regulatory enzyme.

Using gene targeting approach in mouse embryonic stem (ES) cells, we generated an ES cell line unable to produce G6PD (G6pdΔ). G6pdΔ ES cells proliferate in vitro but are extremely sensitive to oxidative stress. By studying ES cell in vitro differentiation, we found that G6PD is indispensable for definitive erythropoiesis.

To characterize how oxidative stress may influence neuronal development, we are investigating the possibility to differentiate G6pdΔES cells into neuronal cells. Moreover, neuronal differentiated G6pdΔ cells could be a tool to test in vitro new drugs able to interfere with the molecular mechanisms of oxidative stress mediated neuronal degeneration.

SHORT CV

Education

1993 Ph. D. in Veterinary Pharmacology and Toxicology Science" at the University of Naples "Federico II

1989 Laurea, "summa cum laude", in Biological Science, University of Naples "Federico II".

Research experience and professional:

Since 2011 Scientific Consultant IRCCS Neuromed, Pozzilli (IS) Italy.Studynig the rule of oxidative-stress in Rett Syndrome.

Since 2007 CNR Senior Researcher

Since 2004 Scientific Coordinator of the Stem Cell Fate Lab at Institute of Genetics and Biophysics "Adriano Buzzati Traverso"-CNR, Naples, Italy

Since 2001 Group leader at Institute of Genetics and Biophysics "Adriano Buzzati Traverso"-CNR, Naples, Italy. Studying the role of enzymes involved in the defence against oxidative-stress during cell differentiation and development

2001 - 2007 CNR Researcher

1997 - 2001 Telethon scientists at IIGB-CNR, Naples. Studing the role of the NADPH producing enzyme in oxidative-stress

1995 - 1997 Long term EMBO postdoctoral fellow in at IGBMC, Illkirch-C.U. de Strasbourg France. Carrying out embryological and genetic analysis of central nervous system in early mouse development.

1993 - 1995 Postdoctoral at IIGB-CNR, Naples. Studying the molecular genetics of human G6PD.

1989 - 1993 PhD student studying the influence of antiprotozoary drug on G6PD.

1988 Summer student at the RPMS in London. Characterizing, at molecular level, a new human G6PD variant.

1982 Student in Biological Science at the University of Naples "Federico II" in the Department of Genetics, General and Molecular Biology.

Selected Publications

1: Manganelli G., Masullo U., Filosa S. HTS/HCS to Screen Molecules Able to Maintain Embryonic Stem Cell Self-Renewal or to Induce Differentiation: Overview of Protocols (2014) Stem Cell Reviews and Reports 10 (6):802-819

2:De Felice C, Della Ragione F, Signorini C, Leoncini S, Pecorelli A, Ciccoli L, Scalabrì F, Marracino F, Madonna M, Belmonte G, Ricceri L, De Filippis B, Laviola G, Valacchi G, Durand T, Galano JM, Oger C, Guy A, Bultel-Poncé V, Guy J, Filosa S*, Hayek J*, D’Esposito M*. 2014. Oxidative brain damage in Mecp2-mutant murine models of Rett syndrome. (2014) Neurobiol Dis. 68:66-77.

*Co-last authors

3:Poeta L., Fusco F., Drongitis D., Shoubridge C., Manganelli G., Filosa S., Paciolla M., Courtney M., Collombat P., Lioi M.B., Gecz J., Ursini M.V., Miano M.G. A regulatory path associated with X-linked intellectual disability and epilepsy links KDM5C to the polyalanine expansions in ARX. (2013) Am J Hum Genet 92 (1):114-125.

4:Manganelli G., Fico A., Masullo U., Pizzolongo F., Cimmino A., Filosa S. Modulation of the pentose phosphate pathway induces endodermal differentiation in embryonic stem cells. (2012) PLoS One 7 (1):e29321.

5:Fico A., Manganelli G., Cigliano L., Bergamo P., Abrescia P., Franceschi C., Martini G., Filosa S. 2-deoxy-d-ribose induces apoptosis by inhibiting the synthesis and increasing the efflux of glutathione. (2008) Free Radical Biology & Medicine, 45 (2):211-217.

6:Fico A., Manganelli G., Simeone M., Guido S., Minchiotti G., Filosa S. High throughput screening compatible single-step protocol to differentiate ES cells in neurons. (2008) Stem Cells and Development, 17 (3):573-584.

7:Fico A., Paglialunga F., Filosa S. Reply to ’Role of glucose-6-phosphate dehydrogenase for oxidative stress and apoptosis’. (2006). Cell Death Differ. 13(3):529-530

8:Paglialunga F., Fico A., Iaccarino I., Notaro R., Luzzatto L., Martini G., Filosa S. G6PD is indispensable for erythropoiesis after the embryonic-adult hemoglobin switch. (2004) Blood 104(10): 3148-52.

9:Fico A., Paglialunga F., Cigliano L., Abrescia P., Verde P., Martini G., Iaccarino I., Filosa S. Glucose-6-phosphate dehydrogenase plays a crucial role in protection from redox-stress-induced apoptosis. (2004) Cell Death Differ. 11(8):823-31

10:Filosa S., Fico A., Paglialunga F., Balestrieri M., Crooke A., Verde P., Abrescia P., Bautista J.M., Martini G. Failure to increase glucose consumption through the pentose-phosphate pathway results in the death of glucose-6-phosphate dehydrogenase gene-deleted mouse embryonic stem cells subjected to oxidative stress. (2003) Biochem J 370:935-43

 

Selected Publications
(full list available at CNR People)

Page Contents

Related Contents

Other Personal Pages