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Institute of Biosciences and Bioresources

National Research Council of Italy

Maria Teresa Scarano

Role: Researcher
Section: Researchers and Technologists
Division: Portici
Tel: (39) 081-2539025-429
E-mail: mariateresa.scarano@ibbr.cnr.it


BIRTH DATE: 13th December 1962

BIRTHPLACE: Roma, ITALY

NATIONALITY: IT

PRESENT ADDRESS: Via Roma, 35 Campobasso, Italy

MARITAL STATUS: Married with two daughters

EDUCATION: University of Naples

DEGREE: Laurea in Agricultural Science, December 1988 (mark 110/110).

Researcher at the Institute of Biosciences and Bioresources (Research Division of Portici-Napoli) of the National Council of Research, Via Università, 133 (Parco Gussone) 80055 Portici (NA) tel. 081.2539025 - 429, fax 081.7753579.

POSTGRADUATE EXPERIENCE

1989: Consultant Agriconsulting Spa Roma;

Collaborator Studio Tecnico Agronomico, Campobasso;

Teacher "Agricultural Science" at Istituto Tecnico Geometri, Campobasso;

Agronomist Comune di Campodipietra (CB);

1990: Teacher "Agricultural Science" at Istituto Tecnico Agrario, Larino (Campobasso);

1991/1993: Postgraduate Fellowship of National Research Council at the “Institute of Mutagenesi e Differenziamento” in Pisa, about "Tissue culture and isolation of Temperature-sensitive mutants in Daucus carota" working with Prof. Vittoria Nuti Ronchi;

1993: Volunteer at the “Department Scienze Animali Vegetali e dell’Ambiente” in Campobasso, working on "Isolation, characterisation and utilisation of Lactobacillus plantarum strains in biological fermentation of green olives;

1994/1995: Postgraduate Fellowship of National Research Council at the University of California, Davis, CA-USA “Dept. of Environmental Horticulture” about “Somatic embryogenesis in Conifers”, with Prof. Don J. Durzan;

July 1, 1995: Researcher at the “Istituto di Ricerca per la Genetica degli Agrumi” CNR in Palermo, Italy.

1996: Participant in a project, within the scientific cooperation agreement between National Council of research (CNR) and the Chinese Academy of Agricultural Science (CAAS) practising a stage in Cina visiting the “Citrus Research Institute” in Beibei, Sichuan, from October 20 till November 4;

1997: Participant to the program “Biotecnology” organized by the ‘Dipartimento Innovazione’-E.N.E.A., within the Stride-Actinia project, practising short stages in Research Institutes located in France, Netherlands, Belgium and Germany from December 1 till 12;

from DECEMBER 16, 1997: permanent researcher at CNR, at the “Istituto di Ricerca per la Genetica degli Agrumi” CNR in Palermo, Italy. (former Centro di Studio per il Miglioramento Genetico degli Agrumi of Palermo), now named Institute of Plant Genetcs - Research Divison of Palermo;

2003: leader of research project entitled “Somatic hybridization by

protoplast fusion in Arachis hypogea”, within the scientific cooperation agreement between the National Council of Research (CNR) and the Council of Scientific and Industrial Research (CSIR), practiced a stage at the National Chemical Laboratory of Poone (India) from October 4 till 18;

2004:leader of research project entitled “Somatic hybridization by

protoplast fusion in Arachis hypogea”, within the scientific cooperation agreement between the National Council of Research (CNR) and the Council of Scientific and Industrial Research (CSIR), practiced a second stage at the National Chemical Laboratory of Poone (India) from October 1 till 15;

2005-2009: from January temporary transfer at the Department of Animal, Plant and Environmental Science at the University of Molise in Campobasso-Italy where she has worked and carried out projects on genetics of Fir, Grapevine and Olive trees;

2006:Teaching “Animal Genetics” for the Corso di Laurea in Scienze e Tecnologie delle Produzioni Animali of the Faculty of Agricultural Science, Università degli Studi del Molise, Via De Sanctis snc. Campobasso. Length 16 hours, from May 9 till 30;

2011 Moving to Research Division of Portici (NA) where she actually collaborates with the Potato Breeding & Genomics Lab (http://www.pbglab.it) studying the inheritance of nucleic and cytoplasmic traits of potato somatic hybrids and their related androgenetic haploids employing ISSR and PCR-RFLP markers.

from DECEMBER 8, 2013 On maternity leave

LABORATORY EXPERIENCE

  • Plant tissue culture;
  • Chemical mutagenesis and isolation of induced mutants;
  • Basic technique in cytology: Feulgen staining, mitotic cycle analysis, microdensitometric analysis, cryostate and microtome sectioning technique, fluorescence staining, in situ hybridisation;
  • Mono e bi-dimensional electrophoresis of proteins;
  • Western blot;
  • Analysis of isozymes by electrophoresis and histochemical staining;
  • Protoplast fusion and regeneration of somatic hybrids;
  • Molecular analysis with ISSR-PCR, PCR-RFLP, AFLP, SSR
  • Collaboration and preparation of practical exercise of the class "Plant somatic cell culture and regeneration" organised by Scuola di Specializzazione in Biotecnologie Vegetali of Facoltà di Agraria di Pisa;
  • Participation at weekly Journal Club;
  • Participation at “DNA sequencing course” at UC Davis, California, USA;
  • Participation at University classes at UC Davis: Plant Molecular Biology, Cellular Techniques, Cytogenetics
  • Participation at “3d Course of Techniques of “in vitro” culture and molecular biology”; Brescia, Italy
  • Participation at “3d Eurocoure on Sexual Plant Reproduction and Biotechnological Applications”, Siena, Italy
  • Participation at “1st Pratical Course on DNA labelling with Digoxigenin”. Palermo, Italy

ADDITIONAL INFORMATION

LANGUAGES:

English:TOEFL Exame (May-1995). Score: 607;

Certificate in Advance English (June 1998). Score: GRADE C

German Test: Zertificat Deutsch als Fremdsprache (ZDaF) (July 197) passed with: “sehr gut”;

Test: Zentrale Mittelstufenprüfung (ZMP) (July 2000). Score: “gut”.

List of Publications

"Sviluppo di tecnologie avanzate applicate alle piante", un poster sul progetto: "Ottimizzazione delle metodologie per la produzione di aploidi in specie di interesse agrario". Responsabile: G.Martini, Collaboratori: Giorgetti L., Scarano M.-T., Luccarini G., Pitto L., Brunori A., Collina Grenci F., Geri C., Turrini A., Vergara M.R., Colella C., Evangelista M.,.Boschi E, Caltavuturo L., Miarelli C., Nuti Ronchi V. (1993) .Convegno annuale M.A.F. Ischia, 22-26 marzo;

”Auxin induced reproductive organs as prymary response to culture of different plant species”. Giorgetti L., Scarano M.-T., Pitto L., Miarelli C., Luccarini G., Colella C:, Nuti Ronchi V. (poster) (1993). In Vitro 29A:75;

”Iniziale caratterizzazione di un mutante temperatura sensibile per l’embriogenesi in Daucus carota”. Scebba F., Scarano M.-T., Giorgetti L., Luccarini G., Caltavuturo L., Pitto L., Nuti Ronchi V. (poster) (1995). Atti XXXIX Convegno annuale S.I.G.A. Vasto Marina (CH), 27-30 settembre. pagg. 187-188;

“Possibilità d’impiego di Lattobacilli oleuropeinolitici nella deamarizzazione “al naturale” delle olive da mensa”, Ciafardini G., Scarano M.-T., (comunicazione orale). (1993). Atti Convegno su: “Tecniche, norme e qualità in Olivicoltura”. Potenza, 15-17 dicembre. pagg. 295-303;

“Indagine preliminare sulla coltura di microspore isolate in Citrus“, Germanà M.A., Scarano M.-T., Crescimanno F.G., (comunicazione orale). (1996). Atti III Giornate Scientifiche S.O.I. Erice, 10-14 Marzo. pagg. 7-8;

“First results on isolated microspore culture of Citrus”, Germanà M.A., Scarano M.-T., Crescimanno F.G. (poster) 1996. Proc. Int. Soc. Citriculture. Sun City Resort. South Africa, 12-17 Maggio 1996. pagg. 882-885;

“Confronto tra gli oli essenziali estratti dall’ibrido somatico “Valencia + Femminello” e dai parentali”, Fatta Del Bosco S., Palazzolo E., Scarano M.-T., Germanà M.A., Tusa N. (poster). (1996). XL Convegno S.I.G.A. Perugia, 18-21 settembre. pag. 156;

“Aspetti citoistologici sulle prime fasi di differenziazione in vitro di alcuni embrioni di Cipresso (Cupressus sempervirens L.)”, Scarano M.-T., Havel L, Durzan D.J. (poster). (1996). XL Convegno S.I.G.A. Perugia, 18-21 settembre. pag. 176;

“Xylogenesis in Cupressus callus involves apoptosis”, Havel L, Scarano M.-T., Durzan D.J. (1997) Advances in Horticultural Science. 11: 37-40;

“Comparison between essential oil yield and constituents of an allotetraploid somatic hybrid of Citrus and its parents”. Fatta Del Bosco S., Palazzolo E., Scarano M.-T., Germanà MA., Tusa N. (1998). Advances in Horticultural Science. 12: 72-77;

“Costituzione e caratterizzazione per mezzo della ‘SPME’ di ibridi somatici allotetraploidi per il miglioramento genetico del mandarino”. Scarano M.-T., Ferrante S., Ramirez S., Saiano F., Tusa N. (poster). (2000) XLIV Convegno S.I.G.A. Bologna, 20-23 settembre. pag. 135;

“The role of somatic hybridization in Citrus breeding for essential oil”. Fatta Del Bosco S., Ferrante S., Ramirez S., Saiano F., Scarano M.-T. (poster). (2000) XLIV Convegno S.I.G.A. Bologna, 20-23 settembre. pag. 137;

“Somatic hybridization: strategies and results in Citrus cultivar and rootstock improvement”. Tusa N., Scarano M.-T., Abbate L., Ferrante S., Fatta del Bosco S. (comunicazione orale). (2001) XLV Convegno S.I.G.A. Salsomaggiore Terme, 26-29 settembre;

“Flow cytometry analysis and modified AFLP for a rapid characterization of intraspecific hybrid crosses between “Femminello” lemon + “Valencia” orange cybrids and a selection of “Femminello” lemon tolerant to “Malsecco” disease”. Nardi L., Abbate L., Aiello M., Ferrante S., Lucretti S., Scarano M.-T., Tusa N. (poster). (2001) XLV Convegno S.I.G.A. Salsomaggiore Terme, 26-29 settembre;

“Molecular characterization of Citrus symmetric and asymmetric somatic hybrids by means of ISSR-PCR and PCR-RFLP”. Scarano M-T., Abbate L., Ferrante S., Lucretti S., Tusa N. (poster). (2002) XLVI Convegno S.I.G.A. Giardini Naxos, 18-21 settembre;

“Identification of zygotic and nucellar seedlings in Citrus interploid crosses by means of isozymes, flow cytometry and ISSR-PCR”. Tusa N., Abbate L., Ferrante S., Lucretti S., Scarano M.-T. (poster) (2002) XLVI Convegno S.I.G.A. Giardini Naxos, 18-21 settembre;

“ISSR-PCR technique: a useful method for characterizing new allotetraploid somatic hybrids of mandarin”. Scarano M.-T., Abbate L., Ferrante S., Lucretti S., Tusa N. (2002). Plant Cell Reports. 20:1162-1166, doi:10.1007/s00299-002-0450-3;

“Identification of zygotic and nucellar seedlings in Citrus interploid crosses by means of isozymes, flow cytometry and ISSR-PCR”. Tusa N., Abbate L., Ferrante S., Lucretti S., Scarano M.-T. (2002) Cellular and Molecular Biology Letters. 7:703-708;

“Redblush grapefruit + Avana mandarin somatic hybrids. Plantlet leaf essential oil study”. Alonzo G, Ramirez S., Saiano F., Abbate L., Ferrante S., Scarano M.-T., Tusa N. (2002) JEOBP. 5(2): 60-70;

“Applicazioni e risultati dell’ibridazione somatica per fusione di protoplasti nel miglioramento genetico del limone”. Tusa N., Fatta Del Bosco S., Abbate L., Ferrante S., Scarano M.-T. (2002) Atti III Workshop gruppo di lavoro SOI “Lo stato dell’arte nel miglioramento genetico delle principali specie ortoflorofrutticole d’interesse mediterraneo”. Valenzano (BA) 25-26 giugno 2002. 447-452;

“Molecular characterization of Citrus symmetric and asymmetric somatic hybrids by means of ISSR-PCR and PCR-RFLP”. Scarano M.-T., Abbate L., Ferrante S., Lucretti S., Tusa N. (2003). Proceedings of the 10th IAPTC&B Congress, Orlando-Florida, USA. (Ed. I. Vasil) Kluwer Academic Publishers, pp 549-550;

“Flow Cytometry, SSR and modified AFLP markers for the identification of zygotic plantlets in backcrosses between ‘Femminello’ lemon cybrids (2n and 4n) and a diploid clone of ‘Femminello’ lemon (Citrus limon L. Burm. F.) tolerant to mal secco disease”. Scarano M.-T., Tusa N., Abbate L., Lucretti S., Nardi L., Ferrante S. (2003) Plant Science. 164(6): 1009-1017, doi:10.1016/S0168-9452(03)00088-8;

“Identification of zygotic plantlets in backcrosses between ‘Femminello’ lemon cybrids (2n and 4n) and a diploid clone of ‘Femminello’ lemon (Citrus limon L. Burm. F.) tolerant to mal secco disease by means of Flow Cytometry, SSR and modified AFLP markers”. Scarano M.-T., Tusa N., Abbate L., Lucretti S., Nardi L., Ferrante S. (poster) (2003) 7th International Congress of Plant Molecular Biology. Barcelona (Spagna) 23-28 giugno. Book of abstract. Pag. 64;

“Construction of a bacterial artificial chromosome library of a seedless clone of Citrus sinensis cv “Vaniglia”. Ferrante S., Geraci G., Scarano M.-T. (poster) (2003). XLVII Convegno S.I.G.A. Verona, 24-27 settembre;

“SSR and capillary electrophoresis as a tool for distinguishing zygotic plantlets in lemon intraspecific crosses”. Scarano M.-T., Tusa N., Abbate, Graziano D., Reale S., Ferrante S. (poster) (2003). XLVII Convegno S.I.G.A. Verona, 24-27 settembre;

“PCR-based molecular markers approaches in Citrus genetic improvement” Scarano M.-T., Tusa N., Abbate L., Ferrante S., Reale S. (lecture) (2004). Proc. Int. Soc. Citriculture Vol. I: 118-123;

“Construction of a bacterial artificial chromosome library of a seedless clone of Citrus sinensis cv “Vaniglia”. Ferrante S., Geraci G., Scarano M.-T. (poster) (2004). Proc. Int. Soc. Citriculture;

“Somatic hybridization via protoplast fusion and characterization by molecular markers of new promising rootstocks in Citrus”. Ferrante S., Abbate L., Lucretti S., Scarano M.-T., Tusa N. (poster) (2004). XLVIII Convegno S.I.G.A. Lecce, 15-18 settembre;

“Molecular characterization of new citrus tetraploids hybrids by means of SSR markers”. Scarano M.-T., Abbate L., Ferrante S., Lucretti S., Reale S., Tusa N. (poster) (2005). XLIX Convegno S.I.G.A. Potenza, 12-15 settembre;

“Characterization of a novel satellite DNA sequence from Flying Dragon (Poncirus trifoliata)”. De Felice B., Wilson R.R., Ciarmiello L., Scarano M.-T., Ferrante S. (2006). Genetica. 127: 45-53, doi:10.1007/s10709-005-2479-z;

“Spatial distribution of genetic variation in natural Silver fir (Abies alba Lill.) stands based on microsatellite markers.” Scarano M.-T., Buonamici A., Sebastiani F., Pilla F., Reale S., Tognetti R., Vendramin GG. (poster) (2006). XL Convegno S.I.G.A. Ischia, 10-14 settembre;

“Nuclear and cytoplasmic characterization of Citrus asymmetric somatic hybrids by means of ISSR-PCR and CAPS”. Scarano M.-T., Tusa N., Abbate L., Lucretti S., Ferrante S. (poster) (2006). L Convegno S.I.G.A. Ischia, 10-14 settembre;

“Olive autochthonous germplasm of Molise: molecular characterisation by means of SSRs and SNPs”. Reale S., Angiolillo A., Baldoni L., D’Andrea M., Lima G., Scarano M.-T. (poster) (2006). Proceedings of OLIVEBIOTEQ 2006, 2nd International Seminar “Biotechnology and quality of Olive tree products around Mediterranean basin” In Proceedings of second international seminar on biotechnology and quality of olive tree products around the Mediterranean basin, Marsala, Italy (pp. 5-10);

“Construction of a bacterial artificial chromosome library of a seedless clone of Citrus sinensis cv Vaniglia”. Ferrante S., Geraci G., Scarano M.-T. (2007). Italus Hortus 14 (6), 22-23.

“Genetic variability in Trigla lucerna by microsatellite molecular markers isolation”. Pilla F., D’Andrea M., Merigioli A., Scarano M.-T., Forlini L., Roncarati A., Melotti P. (2007). Ital. J. Anim. Sci. Vol. 6 (suppl.1), 192-194;

“Cloning and characterization of genes involved in the production of secondary metabolite in Olea europaea L.”. Reale S., Fierro M., Petrella C., Petrella F., Pilla F., Rao R., De Felice B., Ciarmiello L.F., Scarano M.-T. (poster) (2007). LI Convegno S.I.G.A. Riva del Garda (TN) 23-26 settembre;

“Identification of unreduced gamete origin in Citrus interploid crosses(2X X 4X) by means of SSR markers”. De Patrizio A., Abbate L., Ferrante S., Lucretti S., Reale S., Scarano M.-T., Tusa N. (poster) (2007). LI Convegno S.I.G.A. Riva del Garda (TN) 23-26 settembre;

“Assessment of the origins of Citrus tetraploid hybrids (2n=4X) arising from different interploid crosses (2X x 4X) by means of SSR markers and PCR based dosage effects”. Ferrante S., Abbate L., De Patrizio A., Lucretti S., Reale S., Scarano M.-T., Tusa N. (poster) (2008) 11th International Citrus Congress. Wuhan (China) 26-30 ottobre;

“Cattle breed traceability in meat and cheese using DNA polymorphisms detected by the snapshot method”. D’Andrea M., Merigioli A., Scarano M.-T., Pilla F. (2009). Ital. J. Food Sci. 21: 365-373;

“Assessment of the origin of citrus tetraploid hybrids (2n=4x) arising from different interploid crosses (2x X 4x) by means of SSR markers and PCR based dosage effects.” Ferrante S., Lucretti S., Reale S., De Patrizio A., Abbate L., Tusa N., Scarano M.-T, (2009). Euphytica. 173: 223-233, doi:10.1007/s10681-009-0093-3;

“Morphological and molecular characterization of new putative olive cultivar of Molise, Italy”. D’Andrea M., D’Imperio M., Pilla F., Scarano M.-T., Viscosi V., Zullo B.A. (poster) (2009). LIII Convegno S.I.G.A. Torino 16-19 settembre;

“Integration between molecular and morphological markers for the exploitation olive germplasm (Olea europaea)”. D’Imperio M., Viscosi V., Scarano M.-T., D’Andrea M., Zullo B.A., Pilla F. (2011). Sci. Hort. 130: 229-240, doi:10.1016/j.scienta.2011.06.050;

“Caratterizzazione molecolare mediante marcatori micro satelliti delle principali cultivar di olivo molisane”. Scarano M.-T., D’Andrea M., Pilla F., Lima G. (poster) (2011). Convegno OLVIVA: ‘Ricerca e trasferimento delle innovazioni tecnologiche nel vivaismo olivicolo’. Bari 6-7 giugno;

“Manuale per l’identificazione e il riordino del patrimonio varietale di olivo”. (2011). A cura di: Baldoni L., Famiani F., Caruso T., Loconsole G., Saponari M.. Convegno OLVIVA: ‘Ricerca e trasferimento delle innovazioni tecnologiche nel vivaismo olivicolo’. Bari 6-7 giugno;

“Genomic stability of androgenetic haploids derived from Solanum tuberosum (+) S. bulbocastanum somatic hybrids”. Scarano M.-T., Cristiano L., Tavano R., Frusciante L., Aversano R. (poster) (2011). LV Convegno S.I.G.A. Assisi 19-22 settembre;

“Characterization of the main olive cultivars in Molise region by means of SSR molecular markers”. D’Andrea M., Pilla F., Lima G., Scarano M.-T. (poster) (2011). LV Convegno S.I.G.A. Assisi 19-22 settembre;

“Development of 23 microsatellite arkers for assessing genetic variability in the tub gurnard (Trigla lucerna L.)”. (2012). D’Andrea M., Roncarati A., Melotti P., Scarano M.-T., Pilla F. Animal Genetics. 43: 646-652. doi: 10.1111/j.1365-2052.2012.02319.x;

“Transcription analysis of Solanum commersonii and Solanum bulbocastanum synthetic polyploids”. (2012). Fasano C., Di Matteo A., Scarano M.-T., D’Agostino N., Aversano R., Carputo D. (poster): LVI Convegno S.I.G.A. Perugia 17-20 settembre;

“Impact of ploidy change on secondary metabolites and photochemical efficiency in Solanum bulbocastanum Dun.” (2013). Caruso I., Dal Piaz F., Malafronte M., De Tommasi N., Aversano R., Zottelec C.W., Scarano M.-T., Carputo D. Natural Product Communication. 8:1387-1392;

“Phenotypic and molecular characterization of plants regenerated from non-cryopreserved and cryopreserved wild Solanum lycopersicum Mill. seeds.” (2014). Zevallos B., Cejas I., Engelmann F., Carputo D., Aversano R., Scarano M.-T.; Yanes E., Martínez-Montero, M., Lorenzo J.C. Cryoletters. (in press).

 

Selected Publications
(full list available at CNR People)

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