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Institute of Biosciences and Bioresources

National Research Council of Italy

Immacolata Fiume

Role: CTER-IV
Section: Technicians
Division: Naples
Tel: (39) 081-6132585-081-6132570
E-mail: immacolata.fiume@ibbr.cnr.it


EDUCATION AND TRAINING

1989 Diploma in Biological chemistry, “Istituto Tecnico Industriale Statale “Francesco Giordani”, Naples, Italy.

1992-1994 Study grant International Mass Spectrometry Centre of CNR, Naples, Italy.

WORKING EXPERIENCE

  • from 1th of January 2014 Mass Spectrometry and Proteomic Laboratory-Institute of Biosciences and Bioresources, CNR - UOS of Naples, Italy
  • 2002-2012 Mass Spectrometry and Proteomic Laboratory-Institute of Protein Biochemistry, CNR - Naples, Italy
  • 1994-2002 Intitute of Food Science and Technology, CNR - Avellino, Italy
  • 1990-1994 International Mass Spectrometry Centre, CNR - Naples, Italy

FIELD OF RESEARCH

  • Protein Biochemistry;
  • Protein purification and characterization;
  • Mass Spectrometry and Proteomics;
  • Phosphoproteomics;
  • Glycoproteomics;
  • ITRAQ and ICAT labeling for quantitative mass spectrometry analysis of proteins;
  • Exploiting exosomes for biomarker discovery studies;
  • Proteomics charecterizations of cell secretome and secretion mechanisms;
  • Identification of new diagnostic/prognostic biomarkers, and drug targets for the development of new therapeutic approaches
  • Development of new mass spectrometry-based analytical methods.

INVOLVEMENT IN RESEARCH PROJECTS

  • “Quantitative proteomics for the identification of differentially expressed proteins in cells treated with Daunomycin bioconjugates” CNR-MTA Agreement CNR/MTA agreement -
  • 2010/2012.
  • “Identification of reliable urinary biomarkers of Diabetic Nephrology by means of powerful and complementary proteomics strategyies” Pogetto Società Italiana di Nefrologia (SIN) - Ricercando 2011.
  • “Nuovi networks molecolari per il controllo dell’omeostasi energetica: implicazioni per il diabete di tipo 2 e l’obesità”, MIUR 2010-2013

TECHNICAL PROFICIENCY

  • Isoelectrofocusing, different elecrophoresis techniques (1D & 2D PAGE), Western blotting, , Gaschromatography, High Performance Liquid Chromatography (RP, HILIC, HPAE), Mass Spectrometry (FAB-MS, GC-MS, MALDI-TOF, LC-MS/MS, SELDI-TOFMS), Enzymatic assays.
  • Proteomic bioinformatics: Scaffold, Mascot.
 

Selected Publications
(full list available at CNR People)

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