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Institute of Biosciences and Bioresources

National Research Council of Italy

Giuseppe Perugino

Role: Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132496
E-mail: giuseppe.perugino@ibbr.cnr.it


Name PERUGINO Giuseppe

Address Via Pietro Castellino 111 - 80131, Napoli

Tel. +39 081 6132496

Fax +39 081 6132634

e-mail giuseppe.perugino@ibbr.cnr.it

alternative email g.perugino@icloud.com

Education

  • 2003 PhD Biological Chemistry and Molecular Biology, University of Naples “Federico II”, Faculty of Biological Sciences.
  • 1997 Degree in Biological Sciences, University of Naples “Federico II”, Faculty of Biological Sciences.

Work Experience

  • 2014 - today staff researcher at the Institute of Biosciences and BioResources (IBBR), National Research Council of Italy.
  • 01/01/2008 - 31/12/2013 staff researcher at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.
  • 01/05/2003 - 31/12/2007 research fellowship at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.
  • 01/11/1998 - 31/10/2002 PhD student at the University of Naples “Federico II”, Department of Organic and Biological Chemistry.
  • 15/06/1998 - 31/10/1998 Post lauream fellowship at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.

Keywords

Hyperthermophilic Archaea - thermostable proteins and enzymes - Carbohydrate Active Enzymes - oligosaccharide biosynthesis - DNA repair enzymes - genome stability - biotechnology - protein tags.

Publications

  • MATTOSSOVICH R, MERLO R, FONTANA A, d’IPPOLITO G, TERNS MP, WATTS EA, VALENTI A, PERUGINO G (2020). A journey down to hell: new thermostable protein-tags for biotechnology at high temperatures. EXTREMOPHILES, vol. 24, p. 81-91, ISSN: 1431-0651, doi: 10.1007/s00792-019-01134-3
  • DEL PRETE S, MERLO R, VALENTI A, MATTOSSOVICH R, ROSSI M, CARGINALE V, SUPURAN CT, PERUGINO G, CAPASSO C. (2019). Thermostability enhancement of the a-carbonic anhydrase from Sulfurihydrogenibium yellowstonense by using the anchoring-and-self-labelling- protein-tag system (ASLtag). JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 34, p. 946-954, ISSN: 1475-6366, doi: 10.1080/14756366.2019.1605991
  • LO GULLO G, MATTOSSOVICH R, PERUGINO G, LA TEANA A, LONDEI P, BENELLI D (2019). Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate. ARCHAEA, vol. 1, p. 1-10, ISSN: 1472-3654, doi: 10.1155/2019/9848253
  • MERLO R, DEL PRETE S, VALENTI A, MATTOSSOVICH R, CARGINALE V, SUPURAN CT, CAPASSO C, PERUGINO G (2019). An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 34, p. 490-499, ISSN: 1475-6366, doi: 10.1080/14756366.2018.1559161
  • PERUGINO G, STRAZZULLI A, MAZZONE M, ROSSI M, MORACCI M (2019). Effects of Random Mutagenesis and In Vivo Selection on the Specificity and Stability of a Thermozyme. CATALYSTS, vol. 9, p. 440-454, ISSN: 2073-4344, doi: 10.3390/catal9050440
  • SERPE M, FORENZA C, ADAMO A, RUSSO N, PERUGINO G, CIARAMELLA M, VALENTI A (2019). the DNA Alkylguanine DNA Alkyltransferase-2 (AGT-2) Of Caenorhabditis Elegans Is Involved In Meiosis And early Development Under physiological Conditions. SCIENTIFIC REPORTS, vol. 9, p. 6889-6903, ISSN: 2045-2322, doi: 10.1038/s41598-019-43394-1
  • STRAZZULLI A, PERUGINO G, MAZZONE M, ROSSI M, WITHERS SG, MORACCI M (2019). Probing the role of an invariant active site His in family GH1 ß-glycosidases. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol. 34, p. 973-980, ISSN: 1475-6366, doi: 10.1080/14756366.2019.1608198
  • BETTOTTI P, VISONE V, LUNELLI L, PERUGINO G, CIARAMELLA M, VALENTI A (2018). Structure and Properties of DNA Molecules Over The Full Range of Biologically Relevant Supercoiling States. SCIENTIFIC REPORTS, vol. 8, p. 1-14, ISSN: 2045-2322, doi: 10.1038/s41598-018-24499-5
  • ROSSI F, MORRONE C, MASSAROTTI A, FERRARIS DM, VALENTI A, PERUGINO G, MIGGIANO G (2018). Crystal structure of a thermophilic O6-alkylguanine-DNA alkyltransferase-derived self-labeling protein-tag in covalent complex with a fluorescent probe. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 500, p. 698-703, ISSN: 0006-291X, doi: 10.1016/j.bbrc.2018.04.139
  • MIGGIANO R, VALENTI A, FRANCA R, RIZZI M, PERUGINO G, CIARAMELLA M. (2017). Every OGT Is Illuminated ... by Fluorescent and Synchrotron Lights. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 18, p. 1-18, ISSN: 1422-0067, doi: 10.3390/ijms18122613
  • MORRONE C, MIGGIANO R, SERPE M, MASSAROTTI A., VALENTI A, DEL MONACO G, ROSSI M, ROSSI F, RIZZI M, PERUGINO G, CIARAMELLA M (2017). Interdomain interactions rearrangements control the reaction steps of a thermostable DNA alkyltransferase. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, vol. 1861, p. 86-96, ISSN: 0304-4165, doi: 10.1016/j.bbagen.2016.10.020
  • VISONE V, HAN W, PERUGINO G, DEL MONACO G, SHE Q, ROSSI M, VALENTI A, CIARAMELLA M.¿ (2017). In vivo and in vitro protein imaging in thermophilic archaea by exploiting a novel protein tag. PLOS ONE, vol. 12, p. 1-19, ISSN: 1932-6203, doi: journal.pone.0185791
  • MIGGIANO R, PERUGINO G, CIARAMELLA M, SERPE M, REJMAN D, PAV O, POHL R, GARAVAGLIA S, LAHIRI S, RIZZI M, and ROSSI F (2016). Crystal structure of Mycobacterium tuberculosis O6-methylguanine-DNA methyltransferase protein clusters assembled onto damaged DNA. BIOCHEMICAL JOURNAL, vol. 473, p. 123-133, ISSN: 0264-6021, doi: 10.1042/BJ20150833
  • VETTONE A, SERPE M, HIDALGO A, BERENGUER J, DEL MONACO G, VALENTI A, ROSSI M, CIARAMELLA M, PERUGINO G (2016). A novel thermostable protein-tag: optimization of the Sulfolobus solfataricus DNA-alkyl- transferase by protein engineering. EXTREMOPHILES, vol. 20, p. 1-13, ISSN: 1431-0651, doi: 10.1007/s00792-015-0791-9
  • PERUGINO G, MIGGIANO R, SERPE M, VETTONE A, VALENTI A, LAHIRI S, ROSSI F, ROSSI M, RIZZI M, and CIARAMELLA M (2015). Structure-function relationships governing activity and stability of a DNA alkylation damage repair thermostable protein. NUCLEIC ACIDS RESEARCH, vol. 43, p. 8801-8816, ISSN: 1362-4962, doi: 10.1093/nar/gkv774
  • JAMROZE A, PERUGINO G, VALENTI A, RASHID N, ROSSI M, AKHTAR M, and CIARAMELLA M (2014). The reverse gyrase form Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, p. 3231-3243, ISSN: 0021-9258, doi: 10.1074/jbc.M113.517649
  • VETTONE A, PERUGINO G, ROSSI M, VALENTI A, and CIARAMELLA M (2014). Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase. EXTREMOPHILES, vol. 18, p. 895-904, ISSN: 1431-0651, doi: 10.1007/s00792-014-0662-9
  • VISONE V, VETTONE A, SERPE M, VALENTI A, PERUGINO G, ROSSI M, and CIARAMELLA M (2014). Chromatin Structure and Dynamics in Hot Environments: Architectural Proteins and DNA Topoisomerases of Thermophilic Archaea. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 15, p. 17162-17187, ISSN: 1422-0067, doi: 10.3390/ijms150917162
  • MIGGIANO R, CASAZZA V, GARAVAGLIA S, CIARAMELLA M, PERUGINO G, RIZZI M, and ROSSI F. (2013). Biochemical and structural studies in the M. tubercolosis O6-methylguanine methyltransferase and mutated variants.. JOURNAL OF BACTERIOLOGY, vol. 195, p. 2728-2736, ISSN: 1098-5530, doi: 10.1128/JB.02298-12
  • COBUCCI-PONZANO B, PERUGINO G, STRAZZULLI A, ROSSI M, and MORACCI M (2012). Thermophilic Glycosynthases for Oligosaccharides Synthesis. METHODS IN ENZYMOLOGY, vol. 510, p. 273-300, ISSN: 0076-6879, doi: 10.1016/B978-0-12-415931-0.00015-X
  • PERUGINO G, VETTONE A, ILLIANO G, VALENTI A, FERRARA MC, ROSSI M, and CIARAMELLA M. (2012). Activity and Regulation of an Archaeal DNA-Alkyltransferase: a Conserved Protein Involved in Repair of DNA Alkylation Damage. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, p. 4222-4231, ISSN: 0021-9258
  • VALENTI A, DE FELICE M, PERUGINO G, BIZARD A, NADAL M, ROSSI M, and CIARAMELLA M. (2012). Synergic and opposing activities of thermophilic RecQ-like helicase and Topoisomerase 3 in Holliday junction processing and replication fork stabilization.. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, p. 30282-30295, ISSN: 0021-9258, doi: 10.1074/jbc.M112.366377
  • COBUCCI-PONZANO B, PERUGINO G, ROSSI M, and MORACCI M (2011). Engineering the stability and the activity of a glycoside hydrolase. PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 24 (1-2), p. 21-26, ISSN: 1741-0126
  • VALENTI A, PERUGINO G, ROSSI M, and CIARAMELLA M (2011). Positive supercoiling in thermophiles and mesophiles: of the good and evil. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 39, p. 58-63, ISSN: 0300-5127, doi: 10.1042/BST0390058
  • Cobucci Ponzano, Beatrice, PERUGINO, GIUSEPPE, Trincone, Antonio, Mazzone, Marialuisa, DI LAURO, BARBARA, Giordano, Assunta, ROSSI, MOSE’, MORACCI, Marco (2010). Applications in Biocatalysis of Glycosyl Hydrolases from the Hyperthermophilic Archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 215-221, ISSN: 1024-2422, doi: 10.1080/10242420310001618555
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G, and ROSSI M (2010). Ischia (Naples) hosted the Eigth Carbohydrate Bioengineering Meeting on May 10-13, 2009. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 28, p. 156, ISSN: 1024-2422
  • PERUGINO G, MORACCI M, and ROSSI M (2010). THERMOPHILIC ENZYMES OF POTENTIAL INDUSTRIAL USE: STRUCTURE AND FUNCTION. In: PECHKOVA E. AND RIEKEL C.. SYNCHROTON RADIATION AND STRUCTURAL PROTEOMICS (Pan Stanford Series on Nanobiotechnology, Series Editor Prof. Nicolini C.). vol. 3, p. 319-355
  • PERUGINO G, VALENTI A, ROSSI M, and CIARAMELLA M (2010). NUOVE PROTEINE TERMOSTABILI PER LA MARCATURA SPECIFICA DI PROTEINE IN VIVO E IN VITRO. NA2010A000013, Consiglio Nazionale delle Ricerche
  • VALENTI A, PERUGINO G, VARRIALE A, D’AURIA S, ROSSI M, and CIARAMELLA M (2010). The Archaeal Topoisomerase Reverse Gyrase Is a Helix-destabilizing Protein That Unwinds Four-way DNA Junctions. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285, p. 36532-36541, ISSN: 0021-9258
  • PERUGINO G, VALENTI A, D’AMARO A, ROSSI M, and CIARAMELLA M (2009). Reverse gyrase and genome stability in hyperthermophilic organisms. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 37, p. 69-73, ISSN: 0300-5127
  • VALENTI A, PERUGINO G, NOHMI T, ROSSI M, and CIARAMELLA M (2009). Inhibition of translesion DNA polymerase by archaeal reverse gyrase. NUCLEIC ACIDS RESEARCH, vol. 37, p. 4287-4295, ISSN: 0305-1048, doi: 10.1093/nar/gkp386
  • DI LAURO B, STRAZZULLI A, PERUGINO G, LA CARA F, BEDINI E, CORSARO MM, ROSSI M, and MORACCI M (2008). Isolation and characterization of a new family 42 ß-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues. BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1784, p. 292-301, ISSN: 0006-3002, doi: 10.1016/j.bbapap.2007.10.013
  • VALENTI A, PERUGINO G, D’AMARO A, CACACE A, NAPOLI A, ROSSI M, and CIARAMELLA M (2008). Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine. NUCLEIC ACIDS RESEARCH, vol. 36, p. 4587-4597, ISSN: 0305-1048
  • AUSILI A, COBUCCI-PONZANO B, DI LAURO B, D’AVINO R, PERUGINO G, BERTOLI E, SCIRÈ A, ROSSI M, TANFANI F, and MORACCI M (2007). A comparative infrared spectroscopic study of glycoside hydrolases from extremophilic archaea revealed different molecular mechanisms of adaptation to high temperatures. PROTEINS, vol. 67, p. 991-1001, ISSN: 0887-3585, doi: doi: 10.1002/prot.21368
  • Ausili, Alessio, Cobucci Ponzano, Beatrice, DI LAURO, BARBARA, D’Avino, Rossana, PERUGINO, GIUSEPPE, Bertoli, Enrico, Scirè, Andrea, ROSSI, MOSE’, Tanfani, Fabio, MORACCI, Marco (2007). A comparative infrared spectroscopic study of glycoside hydrolases from extremophilic archaea revealed different molecular mechanisms of adaptation to high temperatures. PROTEINS, vol. 67, p. 991-1001-1001, ISSN: 0887-3585, doi: 10.1002/prot.21368
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G, and ROSSI M (2007). Biotechnology. In: RICARDO CAVICCHIOLI. Archaea: molecular and cellular biology. p. 478-495, WASHINGTON, DC 20036-2904:America Society for Microbiology
  • GLOSTER TM, ROBERTS S, PERUGINO G, ROSSI M, MORACCI M, PANDAY N, TERINEK M, VASELLA A, and DAVIES GJ (2006). Structural, kinetic, and thermodynamic analysis of glucoimidazole-derived glycosidase inhibitors. BIOCHEMISTRY, vol. 45, p. 11879-11884, ISSN: 0006-2960
  • PERUGINO G, FALCICCHIO P, CORSARO MM, MATSUI I, PARRILLI M, ROSSI M, and MORACCI M (2006). Preparation of a glycosynthase from the b-glycosidase of the hyperthermophilic Archaeon Pyrococcus horikoshii. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 24, p. 23-29, ISSN: 1024-2422
  • COBUCCI-PONZANO B, PERUGINO G, DI LAURO B, MAZZONE M, ROSSI M, and MORACCI M (2005). Glycoside hydrolases from hyperthermophilic Archaea. In: Biocatalysis: Chemistry and Biology. p. 127-149, Eds Research Signpost
  • FALABELLA P, PERUGINO G, CACCIALUPI P, RIVIELLO L, VARRICCHIO P, TRANFAGLIA A, ROSSI M, MALVA C, GRAZIANI F, MORACCI M, and PENNACCHIO F (2005). A novel Fatty Acid Binding Protein produced by teratocytes of the aphid parasitoid Aphidius ervi. INSECT MOLECULAR BIOLOGY, vol. 14, p. 195-205, ISSN: 0962-1075
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G, GIORDANO A, TRINCONE A, and ROSSI M (2005). Recent developments in the synthesis of oligosaccharides by hyperthermophilic glycosidases. In: Handbook of Carbohydrate Engineering. Eds Taylor and Francis Group
  • PERUGINO G, COBUCCI-PONZANO B, ROSSI M, and MORACCI M (2005). Recent advances in the oligosaccaride synthesis promoted by catalytically engineered glycosidases. ADVANCED SYNTHESIS & CATALYSIS, vol. 347, p. 941-950, ISSN: 1615-4150
  • TRINCONE A, GIORDANO A, PERUGINO G, ROSSI M, and MORACCI M (2005). Highly productive autocondensation and transglycosylation reactions with Sulfolobus solfataricus glycosynthase. CHEMBIOCHEM, vol. 6, p. 1431-1437, ISSN: 1439-4227
  • GLOSTER TM, ROBERTS S, DUCROS V.M, PERUGINO G, ROSSI M, HOOS R, MORACCI M, VASELLA A, and DAVIES GJ (2004). Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors. BIOCHEMISTRY, vol. 43, p. 6101-6109, ISSN: 0006-2960
  • PERUGINO G, TRINCONE A, ROSSI M, and MORACCI M (2004). Oligosaccharide synthesis by glycosynthases. TRENDS IN BIOTECHNOLOGY, vol. 22, p. 31-37, ISSN: 0167-7799
  • COBUCCI-PONZANO B, PERUGINO G, TRINCONE A, MAZZONE M, DI LAURO B, GIORDANO A, ROSSI M, MORACCI M (2003). Applications in Biocatalysis of Glycosyl Hydrolases from the Hyperthermophilic Archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 215-221, ISSN: 1029-2446, doi: 10.1080/10242420310001618555
  • Cobucci Ponzano, Beatrice, PERUGINO, GIUSEPPE, Trincone, Antonio, Mazzone, Marialuisa, DI LAURO, BARBARA, Giordano, Assunta, ROSSI, MOSE’, MORACCI, Marco (2003). Applications in Biocatalysis of Glycosyl Hydrolases from the Hyperthermophilic Archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 215-221, ISSN: 1024-2422, doi: 10.1080/10242420310001618555
  • PERUGINO G, TRINCONE A, GIORDANO A, VAN DER OOST J, KAPER T, ROSSI M, and MORACCI M (2003). The activity of hypethermophilic glycosynthases is significantly enhanced at acidic pH. BIOCHEMISTRY, vol. 42, p. 8484-8493, ISSN: 0006-2960
  • TRINCONE A, GIORDANO A, PERUGINO G, COBUCCI-PONZANO B, ROSSI M, and MORACCI M (2003). Glycosyl hydrolases from Sulfolobus solfataricus: enzymology and applications. In: Recent Research Developments in Biotechnology & Bioengineering. p. 129-145, Eds Research Signpost
  • TRINCONE A, GIORDANO A, PERUGINO G, ROSSI M, and MORACCI M (2003). Glycosynthase-catalysed syntheses at pH below neutrality. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 13, p. 4039-4042, ISSN: 0960-894X
  • TRINCONE A, PAGNOTTA E, GIORDANO A, PERUGINO G, ROSSI M, and MORACCI M (2003). Enzymatic synthesis of 2-deoxyglycosides using the beta-glycosidase of the archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21, p. 17-24, ISSN: 1024-2422
  • MORACCI M, TRINCONE A, COBUCCI-PONZANO B, PERUGINO G, CIARAMELLA M, and ROSSI M (2001). Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases from Sulfolobus solfataricus. EXTREMOPHILES, vol. 5, p. 145-152, ISSN: 1431-0651
  • POUWELS J, MORACCI M, COBUCCI-PONZANO B, PERUGINO G, VAN DER OOST J, KAPER T, LEBBINK J.H, DE VOS W.M, CIARAMELLA M, and ROSSI M (2000). Activity and stability of hyperthermophilic enzymes: a comparative study on two archaeal beta-glycosidases. EXTREMOPHILES, vol. 3, p. 157-164, ISSN: 1431-0651
  • TRINCONE A, PERUGINO G, ROSSI M, and MORACCI M (2000). A novel thermophilic glycosynthase that effects branching glycosylation. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 10, p. 365-368, ISSN: 0960-894X
  • MORACCI M, TRINCONE A, PERUGINO G, CIARAMELLA M, and ROSSI M (1998). Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles. BIOCHEMISTRY, vol. 37, p. 17262-17270, ISSN: 0006-2960
 

Selected Publications
(full list available at CNR People)

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