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Institute of Biosciences and Bioresources

National Research Council of Italy

Giuseppe Perugino

Role: Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132496
E-mail: giuseppe.perugino@ibbr.cnr.it


Name PERUGINO Giuseppe

Address Via Pietro Castellino 111 - 80131, Napoli

Tel. +39 081 6132496

Fax +39 081 6132634

e-mail giuseppe.perugino@ibbr.cnr.it

Education

  • 2003 PhD Biological Chemistry and Molecular Biology, University of Naples “Federico II”, Faculty of Biological Sciences.
  • 1997 Degree in Biological Sciences, University of Naples “Federico II”, Faculty of Biological Sciences.

Work Experience

  • 2014 - today staff researcher at the Institute of Biosciences and BioResources (IBBR), National Research Council of Italy.
  • 01/01/2008 - 31/12/2013 staff researcher at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.
  • 01/05/2003 - 31/12/2007 research fellowship at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.
  • 01/11/1998 - 31/10/2002 PhD student at the University of Naples “Federico II”, Department of Organic and Biological Chemistry.
  • 15/06/1998 - 31/10/1998 Post lauream fellowship at the Institute of Protein Biochemistry (IBP), National Research Council of Italy.

Keywords

Hyperthermophilic Archaea - thermostable proteins and enzymes - Carbohydrate Active Enzymes - oligosaccharide biosynthesis - DNA repairing enzymes - genome stability -protein tags.

Selected Publications

  • VETTONE A, PERUGINO G., ROSSI M, VALENTI A, and CIARAMELLA M (2014). Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase. EXTREMOPHILES, ISSN: 1431-0651, doi: 10.1007/s00792-014-0662-9
  • VISONE V, VETTONE A, SERPE M, VALENTI A, PERUGINO G., ROSSI M, and CIARAMELLA M (2014). Chromatin Structure and Dynamics in Hot Environments: Architectural Proteins and DNA Topoisomerases of Thermophilic Archaea. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 15; p. 17162-17187, ISSN: 1422-0067, doi: 10.3390/ijms150917162
  • JAMROZE A, PERUGINO G., VALENTI A, RASHID N, ROSSI M, AKHTAR M, and CIARAMELLA M (2013). The reverse gyrase form Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289; p. 3231-3243, ISSN: 0021-9258, doi: 10.1074/jbc.M113.517649
  • MIGGIANO R, CASAZZA V, GARAVAGLIA S, CIARAMELLA M, PERUGINO G., RIZZI M, and ROSSI F. (2013). Biochemical and structural studies in the M. tubercolosis O6-methylguanine methyltransferase and mutated variants. JOURNAL OF BACTERIOLOGY, vol. 195; p. 2728-2736, ISSN: 1098-5530, doi: 10.1128/JB.02298-12
  • VALENTI A, DE FELICE M, PERUGINO G., BIZARD A, NADAL M, ROSSI M, and CIARAMELLA M. (2012). Synergic and opposing activities of thermophilic RecQ-like helicase and Topoisomerase 3 in Holliday junction processing and replication fork stabilization. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287; p. 30282-30295, ISSN: 0021-9258, doi: 10.1074/jbc.M112.366377
  • PERUGINO G., VETTONE A, ILLIANO G, VALENTI A, FERRARA MC, ROSSI M, and CIARAMELLA M. (2012). Activity and Regulation of an Archaeal DNA-Alkyltransferase: a Conserved Protein Involved in Repair of DNA Alkylation Damage. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287; p. 4222-4231, ISSN: 0021-9258
  • COBUCCI-PONZANO B, PERUGINO G., STRAZZULLI A, ROSSI M, and MORACCI M (2012). Thermophilic Glycosynthases for Oligosaccharides Synthesis. METHODS IN ENZYMOLOGY, vol. 510; p. 273-300, ISSN: 0076-6879, doi: 10.1016/B978-0-12-415931-0.00015-X
  • COBUCCI-PONZANO B, PERUGINO G., ROSSI M, and MORACCI M (2011). Engineering the stability and the activity of a glycoside hydrolase. PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 24 (1-2); p. 21-26, ISSN: 1741-0126
  • VALENTI A, PERUGINO G., ROSSI M, and CIARAMELLA M (2011). Positive supercoiling in thermophiles and mesophiles: of the good and evil. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 39; p. 58-63, ISSN: 0300-5127, doi: 10.1042/BST0390058
  • PERUGINO G., MORACCI M, and ROSSI M (2010). THERMOPHILIC ENZYMES OF POTENTIAL INDUSTRIAL USE: STRUCTURE AND FUNCTION. In: PECHKOVA E. AND RIEKEL C.. SYNCHROTON RADIATION AND STRUCTURAL PROTEOMICS (Pan Stanford Series on Nanobiotechnology, Series Editor Prof. Nicolini C.). vol. 3, p. 319-355,
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G., and ROSSI M (2010). Ischia (Naples) hosted the Eigth Carbohydrate Bioengineering Meeting on May 10-13, 2009. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 28; p. 156-156, ISSN: 1024-2422
  • VALENTI A, PERUGINO G., VARRIALE A, D’AURIA S, ROSSI M, and CIARAMELLA M (2010). The Archaeal Topoisomerase Reverse Gyrase Is a Helix-destabilizing Protein That Unwinds Four-way DNA Junctions. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285; p. 36532-36541, ISSN: 0021-9258
  • PERUGINO G., VALENTI A, ROSSI M, and CIARAMELLA M (2010). NUOVE PROTEINE TERMOSTABILI PER LA MARCATURA SPECIFICA DI PROTEINE IN VIVO E IN VITRO. NA2010A000013. Consiglio Nazionale delle Ricerche
  • PERUGINO G., VALENTI A, D’AMARO A, ROSSI M, and CIARAMELLA M (2009). Reverse gyrase and genome stability in hyperthermophilic organisms. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 37; p. 69-73, ISSN: 0300-5127
  • VALENTI A, PERUGINO G., NOHMI T, ROSSI M, and CIARAMELLA M (2009). Inhibition of translesion DNA polymerase by archaeal reverse gyrase. NUCLEIC ACIDS RESEARCH, vol. 37; p. 4287-4295, ISSN: 0305-1048, doi: 10.1093/nar/gkp386
  • PERUGINO G., VALENTI A, D’AMARO A, CACACE A, NAPOLI A, ROSSI M, and CIARAMELLA M (2008). Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine. NUCLEIC ACIDS RESEARCH, vol. 36; p. 4587-4597, ISSN: 0305-1048
  • DI LAURO B, STRAZZULLI A, PERUGINO G., LA CARA F, BEDINI E, CORSARO MM, ROSSI M, and MORACCI M (2008). Isolation and characterization of a new family 42 ß-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues. BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1784; p. 292-301, ISSN: 0006-3002
  • AUSILI A, COBUCCI-PONZANO B, DI LAURO B, D’AVINO R, PERUGINO G., BERTOLI E, SCIRÈ A, ROSSI M, TANFANI F, and MORACCI M (2007). A comparative infrared spectroscopic study of glycoside hydrolases from extremophilic archaea revealed different molecular mechanisms of adaptation to high temperatures. PROTEINS, vol. 67; p. 991-1001, ISSN: 0887-3585
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G., and ROSSI M (2007). Biotechnology. In: RICARDO CAVICCHIOLI. Archaea: molecular and cellular biology. p. 478-495, WASHINGTON, DC 20036-2904: America Society for Microbiology
  • PERUGINO G., FALCICCHIO P, CORSARO MM, MATSUI I, PARRILLI M, ROSSI M, and MORACCI M (2006). Preparation of a glycosynthase from the b-glycosidase of the hyperthermophilic Archaeon Pyrococcus horikoshii. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 24; p. 23-29, ISSN: 1024-2422
  • GLOSTER TM, ROBERTS S, PERUGINO G., ROSSI M, MORACCI M, PANDAY N, TERINEK M, VASELLA A, and DAVIES GJ (2006). Structural, kinetic, and thermodynamic analysis of glucoimidazole-derived glycosidase inhibitors. BIOCHEMISTRY, vol. 45; p. 11879-11884, ISSN: 0006-2960
  • MORACCI M, COBUCCI-PONZANO B, PERUGINO G., GIORDANO A, TRINCONE A, and ROSSI M (2006). Recent developments in the synthesis of oligosaccharides by hyperthermophilic glycosidases. Handbook of Carbohydrate Engineering. Eds Taylor and Francis Group
  • COBUCCI-PONZANO B, PERUGINO G., DI LAURO B, MAZZONE M, ROSSI M, and MORACCI M (2005). Glycoside hydrolases from hyperthermophilic Archaea. Biocatalysis: Chemistry and Biology. p. 127-149, Eds Research Signpost
  • FALABELLA P, PERUGINO G., CACCIALUPI P, RIVIELLO L, VARRICCHIO P, TRANFAGLIA A, ROSSI M, MALVA C, GRAZIANI F, MORACCI M, and PENNACCHIO F (2005). A novel Fatty Acid Binding Protein produced by teratocytes of the aphid parasitoid Aphidius ervi. INSECT MOLECULAR BIOLOGY, vol. 14; p. 195-205, ISSN: 0962-1075
  • TRINCONE A, GIORDANO A, PERUGINO G., ROSSI M, and MORACCI M (2005). Highly productive autocondensation and transglycosylation reactions with Sulfolobus solfataricus glycosynthase. CHEMBIOCHEM, vol. 6; p. 1431-1437, ISSN: 1439-4227
  • PERUGINO G., COBUCCI-PONZANO B, ROSSI M, and MORACCI M (2005). Recent advances in the oligosaccaride synthesis promoted by catalytically engineered glycosidases. ADVANCED SYNTHESIS & CATALYSIS, vol. 347; p. 941-950, ISSN: 1615-4150
  • GLOSTER TM, ROBERTS S, DUCROS V.M, PERUGINO G., ROSSI M, HOOS R, MORACCI M, VASELLA A, and DAVIES GJ (2004). Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors. BIOCHEMISTRY, vol. 43; p. 6101-6109, ISSN: 0006-2960
  • PERUGINO G., TRINCONE A, ROSSI M, and MORACCI M (2004). Oligosaccharide synthesis by glycosynthases. TRENDS IN BIOTECHNOLOGY, vol. 22; p. 31-37, ISSN: 0167-7799
  • TRINCONE A, PAGNOTTA E, GIORDANO A, PERUGINO G., ROSSI M, and MORACCI M (2003). Enzymatic synthesis of 2-deoxyglycosides using the beta-glycosidase of the archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21; p. 17-24, ISSN: 1024-2422
  • TRINCONE A, GIORDANO A, PERUGINO G., ROSSI M, and MORACCI M (2003). Glycosynthase-catalysed syntheses at pH below neutrality. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 13; p. 4039-4042, ISSN: 0960-894X
  • COBUCCI-PONZANO B, PERUGINO G., TRINCONE A, MAZZONE M, DI LAURO B, GIORDANO A, ROSSI M, and MORACCI M (2003). Applications in biocatalysis of glycosyl hydrolases from the hyperthermophilic archaeon Sulfolobus solfataricus. BIOCATALYSIS AND BIOTRANSFORMATION, vol. 21; p. 215-221, ISSN: 1024-2422
  • PERUGINO G., TRINCONE A, GIORDANO A, VAN DER OOST J, KAPER T, ROSSI M, and MORACCI M (2003). The activity of hypethermophilic glycosynthases is significantly enhanced at acidic pH. BIOCHEMISTRY, vol. 42; p. 8484-8493, ISSN: 0006-2960
  • TRINCONE A, GIORDANO A, PERUGINO G., COBUCCI-PONZANO B, ROSSI M, and MORACCI M (2003). Glycosyl hydrolases from Sulfolobus solfataricus: enzymology and applications. Recent Research Developments in Biotechnology & Bioengineering. p. 129-145, Eds Research Signpost
  • MORACCI M, TRINCONE A, COBUCCI-PONZANO B, PERUGINO G., CIARAMELLA M, and ROSSI M (2001). Enzymatic synthesis of oligosaccharides by two glycosyl hydrolases from Sulfolobus solfataricus. EXTREMOPHILES, vol. 5; p. 145-152, ISSN: 1431-0651
  • TRINCONE A, PERUGINO G., ROSSI M, and MORACCI M (2000). A novel thermophilic glycosynthase that effects branching glycosylation. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 10; p. 365-368, ISSN: 0960-894X
  • POUWELS J, MORACCI M, COBUCCI-PONZANO B, PERUGINO G., VAN DER OOST J, KAPER T, LEBBINK J.H, DE VOS W.M, CIARAMELLA M, and ROSSI M (2000). Activity and stability of hyperthermophilic enzymes: a comparative study on two archaeal beta-glycosidases. EXTREMOPHILES, vol. 3; p. 157-164, ISSN: 1431-0651
  • MORACCI M, TRINCONE A, PERUGINO G., CIARAMELLA M, and ROSSI M (1998). Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles. BIOCHEMISTRY, vol. 37; p. 17262-17270, ISSN: 0006-2960
 

Selected Publications
(full list available at CNR People)

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