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Institute of Biosciences and Bioresources

National Research Council of Italy

Francesco Carimi

Role: Senior Researcher
Section: Researchers and Technologists
Division: Palermo
Tel: (39) 091-6574578
E-mail: francesco.carimi@ibbr.cnr.it


Head of the Palermo Division of the IBBR/CNR

Curriculum Vitae

Date and Place of Birth: October 4th 1961, Palermo, Italy

Nationality: Italian

Address: Institute of Biosciences and BioResources, Division of Palermo, National Research Council - Corso Calatafimi, 414 - I-90129, Palermo, Italy

Tel: ++39 091 6574578

Fax: ++39 091 423424

E-mail: francesco.carimi@ibbr.cnr.it

Scopus Author ID: 6603794044

WoS Researcher ID: E-2954-2016

Orcid ID: 0000-0002-7700-9527

Education and Training

Master of Science in Plant Science (Final mark of 110/110 with distinction) - University of Palermo, Italy, 1987. Main courses on Plant Biology, Plant Genetics, Plant Physiology, Plant Breeding, Plant Pathology, Agronomy

Skills and Strengths

- Strong management and leadership experience in planning and leading teams of researchers working on project regarding safeguard and sustainable management of bioresources in the agricultural, agro-food, environmental fields, in particular by developing applications aimed at the improvement and appraisal of agro-food and environmental productions.

- Extensive policy and coordination expertise with the transfer of knowledge to national and international scientific institutions in the field of biotechnologies applied to the genetic and sanitary improvement of crops.

- Ability to manage several scientific projects simultaneously, including monitoring, reporting, information flow, as well as laboratory and field activities.

Excellent research and analytical skills. Good oral and written communication skills.

Affiliations

- 2016: Member of Board of Directors of the Italian Network for Research in Organic Agriculture (RIRAB);

- 2010: Member of Scientific Committee of the Italian Technology Platform in Organic Farming PTBio;

- 2008: Coordinator of the Thematic Working Group “Biodiversity” of the Italian network for the research in organic farming (INROF);

- 2002: Member of Consortium of Research, Experimentation and Services for Sicilian Crop Breeding;

- 2001: Member of Research Consortium, Experimentation and Services for Sicilian nursery;

- 2000: Member - Scientific Council of Institute for Research of Citrus Genetics, CNR;

- 1999: Member of Mediterranean Citrus Network on Certification of Citrus;

- 1999: Member of Mediterranean Citrus Network of Food and Agriculture Organization of the United Nations (MeCiNet-FAO);

- 1999: Member of Ialian Society of Agricultural Genetics.

Fellowships and Awards

Two years fellowship award from National Council of Research for "DNA analysis in Citrus” tenable at Max-Planck-Institut für Züchtungsforschung, Germany - January 1990.

One year fellowship award from Angelo e Salvatore Lima Mancuso foundation for “Plant genetic Resources” - March, 1989.

Career Profile and Experience

2014-present - Head of the Section IBBR-PA of the Institute of Biosciences and BioResources of the National Research Council, Italy: Leads a team of researchers conducting molecular and cell biology researches on woody and herbaceous plants for genetic improvement and conservation of the main Mediterranean crops. Supervises PhD and Master theses of affiliated staff at the Institute of Plant Genetics, University of Palermo, University of Padua, CIHEAM IAMB - Mediterranean Agronomic Institute of Bari, University of Tehran and University Bounaama Djilali of Khemis-Miliana Algeria. Main working field is genetics, breeding, and molecular markers for cultivar identification and biotechnologies for genetic and sanitary improvement of different plant species.

2006-2014 - Head of the Section of IGV-PA of the Institute of Plant Genetics of the National Research Council, Italy: Leads a team of researchers conducting experiments on plant genetics.

2003-2005 - Senior Researcher, National Council of Research, c/o University of Padua: Conducts and supervises researches on programmed cell death in model systems at Interdepartmental Research Center for Innovative Biotechnologies (CRIBI) Laboratory, University of Padua.

2000-2002 - Senior Researcher, National Council of Research, Centre for the Study of Biomembranes: Conducts and supervises research on apoptosis in model systems, Padua.

1994-2000 - Researcher, National Council of Research: Research on Citrus genetics at Institute of Citrus Genetics, Palermo.

1991-1994 - Postdoctoral fellow, National Council of Research: Research on Citrus genetics at Institute of Citrus Genetics, Palermo.

1990 - 1991 - Postdoctoral fellow, Max-Planck-Institut für Züchtungsforschung, Cologne, Germany, on DNA marker technologies in plant genomes: Research on molecular marker technologies for citrus was developed under the supervision of the director Prof. Francesco Salamini.

Scientific Activities

  • More than 100 full papers published in International journals [cited more than 2400 times to the present (Google Scholar), with 13 most cited publications receiving more than 50 citations each] and 14 contributions to book chapter. Holds at present an H index of 30 (Google Scholar).
  • Scientific responsible of 24 national and international projects (Coordinator of project or CNR Unit), managing, under his scientific responsibility and supervision, funds amounting to 17.722.097.71 €.
  • Associated editor and member of editorial board of scientific journals.
  • Ad Hoc Reviewer for several scientific journals (Acta Horticulturae; Acta Physiologiae Plantarum; African Journal of Biotechnology; Agriculture; Aquatic Botany; Biologia; BMC Biotechnology; Comptes Rendus Biologies; Electronic Journal of Biotechnology; Federation of European Biochemical Societies letters (FEBS); Genetic Resources and Crop Evolution; International Journal of Molecular Sciences; International Journal of Tropical Biology and Conservation; In Vitro Cellular & Developmental Biology - Plant; Journal of Biotechnology; Journal of Forestry Research; Journal of Plant Growth Regulation; Plant Cell Reports; Plant Genetic Resources; Plant Growth Regulation; Planta; Plant Science; PLOS One; Physiologia Plantarum; Scientia Horticulturae; Spanish Journal of Agricultural Research; South African Journal of Botany; The Scientific World Journal; Tree Genetics & Genomes; VITIS).
  • Invited speaker and chairperson at international and national congresses. More than hundred communications to national and international congresses and conferences.
  • Teaching of international and national courses. Advise and direct national and international Graduate students, PhD students and Post Doc.

Coordinator or Scientific responsible of national and international projects:

  1. M.I.T.O. Innovative Models and Technologies for the quality and safety of Fruit and Vegetables products ( Project n. F/050081/01-03/X32);
  2. LIFE15 NAT/IT/000914 - CALMARSI LIFE - Measures of integrated conservation of Calendula maritima Guss., a rare endangered species of the Sicilian vascular flora;
  3. Collection, conservation and genetic improvement of the germplasm of grapevine minor cultivars (Vitis vinifera ssp. vinifera) and wild grapevine (Vitis vinifera ssp. sylvestris) in Sicily;
  4. Technologies and processes for the improvement of the shelf-life of products in the agri-food sector through the use of innovative edible films based on pectins (PONO1_02286);
  5. DIVIN: Development of innovative technologies for grapevine;
  6. Technological laboratories for the genetic traceability of agri-food supply chains. Sub-project: Genetic Traceability of Sicilian Wines;
  7. Genetic certification of genetic olive oil industry;
  8. Pilot project for training in the field of agri-food biotechnology;
  9. Conservation of the population of Calendula maritima Guss., a threatened species of Sicilian flora;
  10. CISIA: Innovative methods for the Made in Italy Agri-food industry. Sub-project: OR2.1.2 - Advanced tools for the genetic traceability of agro-food productions;
  11. Biological processes and environmental factors involved in the control of vegetative growth, fructification and the quality of olive oil (Olea europaea L.) in super-intensive plants. Subproject: In vitro study of the architecture of the foliage and of the root system of olive cultivars (Olea europea L.) with different potential for vegetative growth;
  12. Me.P. - Design and Development of a Biosensor for the detection in "real-time" of Heavy Metals;
  13. C.S. Virus Capture System;
  14. Tools and technologies for the typing and characterization of oils from Sicilian olives;
  15. Genetic and sanitary improvement of traditional Sicilian grapevine cultivars;
  16. Genetic certification of Sicilian wine;
  17. Comparison of new citrus varieties for the extension of the harvesting period and the production of fresh juices;
  18. Advanced Research in Citrus industry and their Applications;
  19. Production of improved Botrytis-tolerant Sauvignon Blanc clones combining in vitro and mutagenic technology;
  20. Conservation of the relict endemic Zelkova sicula population - Sub-project: Genetic characterization and ex situ conservation of Zelkova sicula;
  21. Improvement of the olive plant nursery: varietal characterization, health and innovation of the nursery technique (OLVIVA) - Sub-project Characterization of Sicilian olive varieties;
  22. Creation of a plant germplasm bank;
  23. Collection and improvement of Sicilian vegetal genetic resources. Subproject: In vitro culture for the conservation of Sicilian plant germplasm threatened by genetic erosion;
  24. Conservation in vivo and in vitro of minor varieties of fruit trees in danger of extinction typical of Mediterranean environments.

Teaching of international and national courses and seminars:

2018 January 15 - February 2: Coordinator of the Course ’Formation sur l’application des normes internationales dans l’analyse, la caracterisation et l’evaluation genetique - Agrumes et vigne’ within the Programme de Cooperation Technique Tuniso-Italien, Programme de Protection de l’Environnement Projet ‘Ressources phytogenetiques tunisiennes mieux conservees et valorisees’ at the Ministere de l’Equipement et de l’Amenagement Territorial et du Developpement Durable Banque Nationale de Genes, Tunis, Tunisia;

2017 October 15 - 19: Teacher of the Course ‘La gestion d’una banque de genes - Agrumes et vigne (1.3.3)’ within the Programme de Coopération Technique Tuniso-Italien, Programme de Protection de l’Environnement Projet ‘Ressources phytogénétiques tunisiennes mieux conservées et valorisées’ at the Ministère de l’Equipement et de l’Aménagement Territorial et du Développement Durable Banque Nationale de Gènes, Tunis, Tunisia;

- 2017 June 17 - 20: Teacher of the Course ‘Evaluation des ressources génétiques - agrumes et vigne (3.2.2)’ within the Programme de Coopération Technique Tuniso-Italien, Programme de Protection de l’Environnement Projet ‘Ressources phytogénétiques tunisiennes mieux conservées et valorisées’ at the Ministère de l’Equipement et de l’Aménagement Territorial et du Développement Durable Banque Nationale de Gènes, Tunis, Tunisia;

- 2015 December 1 - 3: Supervisor of the Course ’Training of Maltese officials on sanitation of plants, in vitro techniques and plant acclimatization’ funded by Ministry for Sustainable Development, Plant Health Directorate - Plant Health Directorate - Plant Biotechnology Centre, Malta;

- 2014 May 1 - November 1: Supervisor of the Training DIPLOMAzia ’Sciences for DIPLOMACY: Multi-sectorial training program’ at IGV-CNR. ’DIPLOMAzia’ is a training multi-sectorial program generated by an agreement between the National Research Council of Italy and the Ministry of Foreign Affairs and International Cooperation aimed at training 70 young graduates and officials from North Africa, Middle East and the Balkan region (Web site: https://www.cnr.it/en/diplomazia-program);

- 2011 April 9 - May 27: Expert Mission in Algiers and Chlef (Algeria) on ‘Techniques of genetic and health improvement of citrus, grapevine and Drupacee’ (Code Activity 3.1.1A) within the Programme de Cooperation ’Plant certification program to improve fruit production in Algeria’, funded by the Italian Ministry of Foreign Affairs - Directorate General for Development Cooperation, organized by the ’Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM);

- 2010 May 30 - June 12: Supervisor of the Course ‘Plant certification program to improve fruit production in Algeria’ internship for the Algerian technicians of the ‘Centre National de Controle et de Certification des semences et plants’ (CNCC-Alger) and of the Institute Technique de l’Arboriculture Fruitière et de la Vigne (ITAFV-Alger) organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM) (Activity Code 3.7.4A and 4.4.2A);

- 2009 March 26: Teacher of the Seminar entitled "Use of in vitro somatic embryogenesis in fruit crop sanitation" as part of the training program ‘Integrated Pest Management of Mediterranean Fruit Tree Crops" organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM);

- 2008 January 31 - February 6: Expert Mission at Jimah Bahla Agricultural Research Station Barkat Sharaf, Oman: ‘Biotechnologies for genetic improvement of crops’ as part of a cooperation program organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM);

- 2007 April 3 - 6: Teacher of the seminar entitled ‘Use of somatic embryogenesis in woody trees’ as part of a training program organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM);

- 2004 December: Teacher of the Advanced Training Course "Technological Innovation for the improvement of production and agri-food processes in SMEs", organized by the Scientific and Technological Park of Sicily S.c.p.a. he gave the following lessons: Use of somatic embryogenesis in Vitis and Citrus; Cytokinins: general inducers of PCD in plants as in animals; Apoptosis in plants; Senescence and Programmed Cell Death in Plants; Somaclonal variability in Plants;

- 2003 June 17: Teacher of the seminar entitled ‘Use of somatic embryogenesis in Citrus and Vitis’ as part of a training program organized by the "Center International de Hautes Etudes Agronomiques Méditerranéennes" (CIHEAM);

- 2003 March 7: Teacher of the seminar entitled ‘Use of biotechnologies for genetic and sanitary improvement’ as part of a training program organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM);

- 2002 March 18 - 23: Expert Mission (TC INS/05/031), Project Title: ‘Mutation breeding of horticultural crops’ Project; Number: C3-INS/5/031-01-01, Counterpart: Ismiyati Sutarto, CRDIRT, BATAN, Jakarta, project Organized by International Atomic Energy Agency (IAEA):

- 2002 March 18: Seminar at the Center for Research and Development of Isotopes and Radiation Technology in Jacarta (Indonesia) entitled ‘Mutation breeding of horticultural crops and tissue culture techniques’;

- 2002 March 19: Seminar at the Agriculture University of Bogor (Indonesia) entitled ‘Citrus tissue culture mutations’;

- 2002 March 20: at the Assessment Institute for Agricultural Technology in Malang (Indonesia) held a seminar entitled ‘Fruit crops and tissue culture mutations’;

- 2002 March 23: Seminar at the University of Tanjungpura in Pontianak (Indonesia) entitled ‘Tissue culture and mutation breeding’;

- 2001 June 7: Teacher of the Seminar entitled ‘The organization of a plant cell and tissue culture laboratory’ as part of a training program organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM);

- 2000 October 30 - November 3: Teacher of the Courses ‘Demonstration on somatic embryogenesis from style stigma for the sanitation, conservation and free exchange of citrus germplasm’ and ‘Strategies for the safe exchange of citrus germplasm’ held in Cairo as part of a training program ‘Advanced techniques for the collection, virus detection, sanitation and conservation of citrus germplasm’ organized by the ‘Center International de Hautes Etudes Agronomiques Méditerranéennes’ (CIHEAM).

Collaborations with the following national and international scientific institutions:

- Algeria - Crop Production & Sustainable Valorisation of Natural Resources Laboratory, University Djilali Bounaama, Khemis-Miliana;

- Algeria - Department of Agronomy, University of Alger;

- Algeria - Faculty of Science of Nature and Life, University Hassiba Benbouali, Chlef;

- Algeria - Institut Technique de l’Arboriculture Fruitière et de la Vigne (ITAFV), Tassala-El Merdja, Birtouta;

- Algeria - Laboratoire de Phytopathologie et Biologie Moléculaire. Ecole Nationale Supérieure d’Agronomie, El Harrach, Alger;

- Algeria - Laboratoire des Cultures in vitro, Ens Kouba, Alger;

- Algeria - Technical Institute of Fruit Tree and Vine (ITAF), Algiers;

- Algeria - University of Chlef, Plant Biotechnology Laboratory, Faculty of Science of Nature and Life, Chlef;

- Austria - Department of Ecology and Evolution, Plant Ecology, University of Salzburg, Hellbrunnerstr. Salzburg;

- Belgium - Bioversity International, Belgian office at KU Leuven;

- Belgium - Laboratory of Tropical Crop Improvement, Katholieke Universiteit Leuven, Leuven;

- China - Key Laboratory of Natural Pesticide and Chemical Biology of Ministry of Education, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou;

- Czech Republic - Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlarska, Brno;

- Czech Republic - Department of Botany, Faculty of Science, Palacky University in Olomouc, Slechtitelu Olomouc;

- Egypt - Central Laboratory of Organic Agriculture, Agricultural Research Center, Giza;

- Egypt - Department of Genetic, Faculty of Agriculture, Ain Shams University, Cairo;

- Egypt - Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo;

- Egypt - Department of Microbiology, Faculty of Agriculture, Minia University, Minia;

- Iran - Department of Horticulture, Faculty of Genetics and breeding of vegetables, Lorestan University, Khorramabad, Lorestan;

- Iran - Department of Horticulture, Faculty of Genetics and breeding of vegetables, University of Tehran, Pakdasht, Tehran;

- Israel - The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat-Gan;

- Italy - Dipartimento Scientifico e Tecnologico, University of Verona, Verona;

- Italy - Bioversity International, Maccarese, Rome;

- Italy - Centre international de hautes études agronomiques méditerranéennes (CIHEAM), Mediterranean Agronomic Institute of Bari;

- Italy - CRA - Centro di Ricerca per la Viticoltura, Conegliano;

- Italy - CRA-ORL, Unita di Ricerca per l’Orticoltura, Montanaso Lombardo;

- Italy - Dipartimento di Biologia Evolutiva e Funzionale, University of Parma, Parma;

- Italy - Dipartimento di Biotecnologie per il Monitoraggio Agroalimentare ed Ambientale, University of Reggio Calabria, Reggio Calabria;

- Italy - Dipartimento Regionale Azienda Foreste Demaniali, Ufficio Provinciale di Siracusa, Siracusa;

- Italy - Regione Siciliana - Dipartimento Interventi Infrastrutturali per l’Agricoltura, Palermo;

- Italy - Research Center, Genetics and Molecular Biology Department (IASMA), San Michele all’Adige;

- Italy - University of Padua, Department of Biology - Genetic and Plant Genomics Section;

- Italy - University of Palermo;

- Japan - Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki cho, Kita gun, Ikenobe;

- New Zealand - New Zealand Institute for Crop & Food Research Limited, Auckland;

- Oman - Jimah Bahla Agricultural Research Station - Barkat Sharaf;

- Romania - Department of Botany University of Cluj Napoca;

- Romania - La Facultatea de Protectia Mediului dell’Università di Oradea;

- Switzerland - Faculté de biologie et médecine Centre intégratif de génomique Quartier UNIL-Sorge, B’timent Génopode, Lausanne;

- Tunisia - Center of Biotechnology of Borj-Cédria. Laboratory of Plant Molecular Physiology, Hammam-Lif;

- Tunisia - Institut National de Recherche Agronomique de Tunisie (INAT), Tunis;

- Turkey - Department of Biology, Faculty of Life Science, Beytepe, Ankara;

- Turkey - Batı Akdeniz Agricultural Research Institute, Antalya;

- Turkey - Central Research Institute for Field Crops, Turkish Ministry of Food, Agriculture and Livestock, Ankara;

- Turkey - Çukurova University, Faculty of Agriculture, Department of Plant Protection, Adana;

- Turkey - Department of Molecular Biology and Genetic, Faculty of Life Sciences, Muğla Sıtkı Koçman University, Muğla;

- Turkey - Departments of Plant Pathology and Molecular Biology, Turkish Ministry of Agriculture and Rural Affairs;

- Turkey - Mugla University Agricultural Vocational School, Fethiye;

- Turkey - Plant Protection Institute (AZMA), Plant Pathology Department, Yüregir, Adana;

- Turkey -West Mediterranean Agricultural Research Institute (BATEM), Plant Pathology and Molecular Biology Departments, Antalya;

- United Kingdom - Conventions and Policy Section, Royal Botanic Gardens, Kew, Richmond, Surrey;

- United Kingdom - National Pollen and Aerobiology Research Unit (NPARU), The University of Worcester, Henwick Grove, Worcester;

- USA - Department of Plant Biology, Carnegie Institute for Science, 260 Panama Street, Stanford, California.

Advise and direct national and international Graduate students, PhD students, Post Doc and Training Course:

- Trifa Snoussi Hager - 2018 - Doctorat en Sciences de la Production Végétale, Université de Tunis, Tunisia; Chargée de Recherche à Institut National de la Recherche Agronomique de Tunisie;

- Raji Mohammad-Reza - 2015-2017 - PhD student in Horticulture-Biotechnology and Molecular Genetics of Horticultural Crops, at the Faculty of Aburaihan Campus, Department of Horticulture Sciences, University of Tehran, Tehran, Iran;

- Haddad Benalia - 2013-2016 - PhD student in Agronomic Science, entitled "Improving the micropropagation and molecular characterization of several varieties of olive (Olea europaea L.)", University Bounaama Djilali of Khemis-Miliana, Algeria;

- Vassallo Maurice - 2015 December 1st - 3rd - Executive Researcher at the Veterinary and Phytosanitary Regulation Department Plant Health Directorate - Plant Biotechnology Centre, Malta. “Training of Maltese officials on sanitation of plants, in vitro techniques and plant acclimatization”;

- Vella Roderick - 2015 December 1st - 3rd - Executive Researcher at the Veterinary and Phytosanitary Regulation Department Plant Health Directorate - Plant Biotechnology Centre, Malta. “Training of Maltese officials on sanitation of plants, in vitro techniques and plant acclimatization”;

- Sharaf Abdoallah - 2014 - Associate Professor at Ain Shams University, Cairo, Egypt. Training within the DIPLOMACY project on biotechnology for the genetic improvement of crops as a Postdoc;

- El Nagar Mahran - 2014 - Assistant Professor at Benha University, Faculty of Agriculture, Kalubia, Egypt. Training within the DIPLOMACY project on biotechnology for the genetic improvement of crops as a Postdoc;

- Meziane Malika - 2009-2010 - Master of Science in Integrated Pest Management of Mediterranean Fruit Crops at CIHEAM/IAMB - Thesis: Improvement of protocols for the elimination of citrus virus and virus-like diseases;

- Baysal Ömür - 2008-2011 - Post Doc. Training in Molecular Biology and Genetics; Professor of Molecular Biology and Genetics Muğla Sıtkı Kocman University Faculty of Science, Muğla, Turkey;

- Carra Angela - 2004-2010 - Research grant, National Research Council of Italy (CNR) - Cell biology research;

- Valenti Marco - 2009-2010 - University Internship - Acquisition of laboratory techniques of in vitro plant cultures;

- Poma Maria Angela - 2009-2010 - University Internship - Plant germplasm conservation;

- Mercati Francesco - 2009-2010 - PhD student in Biology applied to Agri-food and Forest Systems;

- Gristina Alessandro Silvestre - 2008-2010 - PhD student in Mediterranean agro-ecosystems - Morphological and genetic characterization of populations of Capparis spinosa L. in Sicily;

- Fiore Maria Carola - 2007-2010 - International PhD in "Environmental Agronomy" - Genetic evaluation of Sicilian ecotypes, varieties and populations of fennel;

- Siragusa Mirko - 2006-2009 - Research grant, National Research Council of Italy (CNR) - Genetic analysis of Mediterranean crops;

- Radicini Vincenza - 2008 - Degree thesis - Genetic characterization of Zelkova sicula;

- Giurlanda Giuseppa - 2007 - Degree thesis - Research on the identification of chloroplast microsatellites in Olea europea;

- Graffeo Maria - 2006-2007 - University internship - Basic cell culture techniques;

- Giannini Sergio - 2006-2007 - Research grant, National Research Council of Italy (CNR) - Genetic analysis of Mediterranean crops;

- Badalamenti Ornella - 2006-2007 - University internship - Basic cell culture techniques;

- Del Signore Maria Beatrice - 2005 - University internship - Acquisition of basic techniques for the in vitro regeneration of plant species;

- Tulone Alessandro - 2004-2005 - Degree thesis - Plant DNA analysis;

- Fiore Stefania - 2004 - Research grant, National Research Council of Italy (CNR) - In vitro regeneration of plant species;

- De Michele Roberto - 1998-2001 - Degree thesis - Analysis of apoptotic events associated with senescence in plant cells;

- D’Accardo Carmelo - 1997-1999 - Degree thesis - In vitro regeneration of grapevine rootstocks;

- Morgana Carmelina - 1997-1999 - Degree thesis - Somatic embryogenesis and sanitation in Vitis vinifera.

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Scientific responsible of national and international projects

Covered roles, activities and tasks - Period 2010-2018

1) Role: Scientific Coordinator of the project

Project title:

- M.I.T.O. Innovative Models and Technologies for the quality and safety of Fruit and Vegetables products ( Project n. F/050081/01-03/X32)

-M.I.T.O. Modelli Innovativi e Tecnologie per la qualità e la sicurezza dei prodotti Ortofrutticoli (Progetto n. F/050081/01-03/X32)

Type / Financing: "HORIZON2020" PON I&C 2014-2020, FESR 2014/2020 - Decree of funding 4 August 2016 Prot. No. 0004763

Total amount of the Project (€): 3.186.049.76

Financing Amount for IBBR-CNR Unit (€): 1.110.693.75

Other Italian or foreign partners: ENBIOTECH S.r.l., APOFRUIT ITALIA Soc. Coop. Agricola

Activity period: From: 2 January 2017 - To: 31 December 2019

Project Aims: With this project proposal, we intend to carry out industrial research and experimental development activities, aimed at creating new products and new processes. In particular, we intend to develop new diagnostic applications in molecular biology, which are quick and usable in the field, designing and developing new instruments and reagent kits in order to: 1) research and develop new protocols and new agronomic techniques to rationalize the use of plant protection products as a function of detecting or not (in different vegetative periods) plant pathogens and mycotoxins in fruit and vegetables from integrated production; 2) genetically certify some fruit and vegetable products to scientifically demonstrate their varietal belonging; 3) verify the absence, directly during the processing phases of agri-food products ready for direct consumption, of possible pathogens dangerous for the health of consumers; 3) directing research and development activities aimed at developing a new production process for processing cut fruit ready for consumption. The activities foresee a first phase in which (from the IBBR-CNR) the molecular markers will be searched for the genetic identification of the phytopathogens, the microorganisms that release toxins and some fruit and vegetable products.

2) Role: Coordinator of the project Francesco Carimi - Scientific Coordinator Giuseppe Garfi

Project title:

- LIFE15 NAT/IT/000914 - CALMARSI LIFE - Measures of integrated conservation of Calendula maritima Guss., a rare endangered species of the Sicilian vascular flora

Type / Financing: European Commission, LIFE Program, call 2015 - CUP B72F16000700006

Total amount of the Project (€): 1.020.982.00

Financing Amount for IBBR-CNR Unit (€): 831.204.00

Other Italian or foreign partners: Regional Department of the Environment - Sicilian Region

Activity period: From: 1 November 2016 - To: 1 November 2020

Project Aims:

Calendula maritima Guss. is a herbaceous plant species, with annual or short perennial life cycle depending on water availability as well as on the intensity and length of summer water stress. It plays a key functional role within a variety of coastal ecosystems, even affecting their physiognomy. Currently it often takes part to nitrophilous and ruderal communities concerned by high and continuous input of organic nutrients (stranded remains of marine phanerogams and macroalgae, faeces and food leftovers in proximity of seabird colonies, etc.), contributing remarkably to the overall biodiversity of coastal ecosystems. Despite its notable ecological amplitude and its adaptability to disturbed environments, C. maritima has a very discontinuous distribution. In fact, its area has been concerned by strong and continuous regression during the last 150 years, mainly due to habitat fragmentation triggered by intensive anthropogenic pressure. Moreover, recent studies have pointed out that hybridisation with the relative Calendula fulgida Raf. could represent a severe threat for survival of this endangered species. According to IUCN classification criteria C. maritima is ranked to the category “CR” (= Critically Endangered). The main goal of the project is the conservation of C. maritima; more in detail, specific actions aim at safeguarding the existing populations through the removal/mitigation of the main threats, such as: - Reduction and fragmentation of distribution area and further degradation of current habitats - Genetic pollution - Seasonal disturbances related to coastal use - Competition by alien invasive plant species. In order to face the above listed threats a number of actions has been identified, such as: - Reinforcement of some of the most depauperated populations through plant material of certain origin and genetic identity - Establishment of new populations - Promulgation of a protection rule specifically dedicated to the target species and applied on the whole national territory - Shared planning and management policies of sites hosting the target species populations in agreement with local bodies - Promotion of actions aimed at improving awareness of local people and stakeholders concerning the general issues of biodiversity conservation with special regard to C. maritima.

3) Role: Scientific Coordinator of the project for cultivated form Francesco Carimi - Scientific Coordinator for the wild forms Giuseppe Garfi

Project title:

- Collection, conservation and genetic improvement of the germplasm of grapevine minor cultivars (Vitis vinifera ssp. vinifera) and wild grapevine (Vitis vinifera ssp. sylvestris) in Sicily

- Recupero, conservazione e valorizzazione del germoplasma di vitigni minori (Vitis vinifera ssp. vinifera) e vite selvatica (Vitis vinifera ssp. sylvestris) in Sicilia

Type / Financing: Rural development programme for Sicilian Region, Department of Agricultural Resources and Food, Action 2.1.4/2 A, Decree of funding, Prot. Number 1264, 28 March 2013

Total amount of the Project (€): 494.318.97

Financing Amount for IBBR-CNR Unit (€): 494.318.97

Activity period: From: 28 March 2013 - To: 27 March 2018

Project Aims: The aim of the project is the conservation together with genetic and sanitary improvement of grapevine germplasm (Vitis vinifera ssp. vinifera) in Sicily through recovery and ex-situ conservation through the creation of a collection field of the germoplasm of 1) minor vines and / or at risk of extinction and 2) of the autochthonous relicts of wild vine (Vitis vinifera ssp. sylvestris).

The actions to be taken include the following activities: 1) Identification and collection of varieties of minor or endangered vines of domestic life and sylvatic populations in the whole regional territory, including the Circumsicilian islands; 2) Genetic characterization of the germplasm to be conserved; 3) Creation of a collection field for specific demonstration and dissemination actions; 4) Preparation and implementation of a website for dissemination and public consultation purposes; 5) Genetic and sanitary improvement of germplasm using the most modern biotechnologies.

4) Role: Scientific Coordinator of the project

Project title:

- Technologies and processes for the improvement of the shelf-life of products in the agri-food sector through the use of innovative edible films based on pectins (PONO1_02286)

- Tecnologie e processi per il miglioramento della shelf-life dei prodotti del comparto agroalimentare attraverso l’uso di film edibili innovativi a base di pectine (PONO1_02286)

Type / Financing: Ministry of Education, University and Research (MIUR), National Operative Program "Research and Competitiveness 2007-2013" Axis I - Communication for admission to funding, MIUR, Directorate General for Research Coordination and Development, Document number 6046 of 10 June 2011 - Concession Decree Number 834, 26 November 2012

Total amount of the Project (€): 6.691.239.00

Financing Amount for IBBR-CNR Unit (€): 1.553.023.00

Other Italian or foreign partners: IAMC-CNR, IBIM-CNR; ISAFOM-CNR, APOFRUIT Italia Soc. Coop. Agricola, Agroindustry Advanced Technologies S.p.A, CO.Ri.BLA - Consorzio di Ricerca sul Rischio Biologico in Agricoltura

Activity period: From: 1 February 2013 - To: 30 November 2015

Project Aims: The aim of the project is the development and testing of a technologically innovative solution, based on natural components, such as pectins extracted from citrus fruits or some antimicrobial molecules isolated from the same products on which they are applied, capable of significantly improving shelf life of fish and agricultural products. In particular, there will be an innovative gelatinous substance to be applied to the products being tested in the form of an invisible, odourless and tasteless "edible film" capable of slowing down the senescence of the products themselves by increasing their shelf-life. In detail, the studies of the program can be traced back to two macro-areas: design and development of the film production protocol on food products through the creation of the appropriate instrumentation. A procedural level, we will work for the next step starting from the identification of the "best basic pectin mix" to which, thanks to a research piano, adopted natural compounds with antimicrobial action (yeasts and / or peptides) and other natural substances (waxes) with the aim of identifying the best combination in terms of lengthening the shelf-life.

5) Role: Scientific Coordinator of the IBBR-CNR Unit

Project title:

- DIVIN: Development of innovative technologies for grapevine

- DIVIN: Développement des Interventions innovantes sur les cépages de Vignes

Type / Financing: Programme Opérationnel ENPI Italie - Tunisie 2007-2013, Measure 1.1. Development and Integration of Economic Supply Chain; Communication of admission to facilities, Sicilian Region, Presidency, Department of Programmation, Service V - European Territorial Cooperation and Decentralized Development Cooperation, Prot. Number 9315, 22 May 2013

Total amount of the Project (€): 674.107.06

Financing Amount for IBBR-CNR Unit (€): 118.318.31

Other Italian or foreign partners: Istituto per l’Ambiente Marino Costiero (IAMC-CNR); Centre de Biotechnologie de Borj Cédria, Tunisia; Institut National de la Recherche Agronomique, Tunisia; Direction Générale de la Production Agricole Ministère de l’Agriculture et des Ressources Hydrauliques, Tunisia; Associazione Strada del vino Alcamo DOC

Activity period: From: 22 November 2013 - To: 31 July 2016

Project Aims: The DIVIN project aims to support the integration of the wine sector, promoting innovative strategies for the analysis and genetic improvement of the typical vines of Sicily and Tunisia. The project involves the development of common strategies for the recovery and enhancement of the vines. In particular, the following activities were carried out: 1) Comparative analysis of territories and genetic and health characterization of cultivars. 2) Preparation of descriptive cards of the main autochthonous cultivars. 3) Rehabilitation of the main Sicilian and Tunisian native cultivars. 4) Development of protocols for the conservation of cultivars. 5) Planning of protocols for the genetic and health characterization of the main cultivars typical of Tunisia and Sicily. 6) Development of virus elimination protocols from cultivars.

6) Role: Scientific Coordinator of the IBBR-CNR Unit

Project title:

- Technological laboratories for the genetic traceability of agri-food supply chains. Sub-project: Genetic Traceability of Sicilian Wines

- Laboratori tecnologici per la tracciabilità genetica delle filiere agroalimentari. Sottoprogetto: Tracciabilità Genetica dei Vini Siciliani

Type / Financing: Call Sicilian Region Art. 128 of the regional law 12 May 2010, n. 11 and subsequent amendments and additions

Total amount of the Project (€): 80.000.00

Financing Amount for IBBR-CNR Unit (€): 20.000.00

Other Italian or foreign partners: Consorzio Regionale di Ricerca BioEvoluzione Sicilia, Istituto Zooprofilattico Sperimentale della Sicilia

Activity period: From: 19 January 2017 - To: 18 June 2017

Project Aims: The traceability of the products of agri-food chains is becoming increasingly important for the European economy, above all because of the pressure that consumers exercise to better know not only the nutritional value of food, but also the origin and authenticity of raw materials used for their obtainment. The "molecular" traceability, based on the direct verification of the composition of a food to reconstruct the supply chain, is a fundamental tool for imposing the required measures. The targets of molecular traceability are the DNA present in the products. The presence of DNA in food during technological transformations makes its detection easier and more immediate. The following development project aims to develop a genetic traceability system for Sicilian products of the Sicilian wine production chain that can be used by operators and visible to consumers. We aim to create a system that, through the application of molecular genetic technologies, allows us to recognize the origin of grapes and wine.

7) Role: Scientific Coordinator of the project

Project title:

- Genetic certification of genetic olive oil industry

- Campagna pilota per la certificazione genetica della filiera olivicola

Type / Financing: Call Sicilian Region Art. 128 of the regional law 12 May 2010, n. 11 and subsequent amendments and additions

Total amount of the Project (€): 145.000.00

Financing Amount for IBBR-CNR Unit (€): 80.000.00

Other Italian or foreign partners: Consorzio Regionale di Ricerca BioEvoluzione Sicilia

Activity period: From: 10 May 2015 - To: 30 November 2015

Project Aims: For the implementation of the project ’Pilot Campaign for genetic certification of the olive oil sector’, the BES needs to undertake a scientific collaboration with the IBBR, whose activities will be delegated to the genetic certification of the olive oil sector.

Activity carried out: The project aims to support the genetic traceability of the olive oils. The following activities were carried out within the project: a) finding and collecting the genetic databases necessary for the certification of the oil produced; b) planning of a free genetic certification pilot campaign for oils; c) issue of certifications to companies participating in the pilot project; d) development of a Cross-platform software (PC, tablet, smartphone) for results processing, sampling management, online certification release, analysis database management. Results obtained: creation of a platform for the genetic certification of the olive oil supply chain.

8) Role: Scientific Coordinator of the project

Project title:

- Pilot project for training in the field of agri-food biotechnology

- Progetto pilota per la formazione nel settore delle biotecnologie agroalimentari

Type / Financing: Operational Program Objective Convergence 2007-2013, European Social Fund, Sicilian Region - Axis IV - Human Capital: Public Notice n. 1 of January 26, 2012: Strengthening employability in the R & D system; Creation of research spin-offs; Sicilian Region - Regional Department of Education D.D.G. n. 2137 of 13/05/2014

Total amount of the Project (€): 370.000.00

Financing Amount for IBBR-CNR Unit (€): 370.000.00

Activity period: From: 1 October 2014 - To: 31 October 2015

Project Aims: The project involves the training of 9 unemployed young people living in the Sicily region in the field of agri-food biotechnology for: 1) Strengthening and developing the employability of talents with a high level of education and training, supporting their commitment and inclusion in training courses highly customized for the development of innovative business ideas; 2) Disseminate knowledge and transfer of research results by enhancing local human potential; 3) Strengthen online activities for research and technology transfer between research centres and companies; 4) Promote the work orientation of young people towards the research and innovation system and the creation of businesses in which the research and technology transfer sector is engaged.

Activity carried out: As part of the project, the following activities were carried out for the development of the training course in the cellular and molecular biotechnology sector applied to the main sectors of the Sicilian agri-food sector. The training course also dealt with the following thematic areas: a) Business planning; b) Organization of research and development activities; c) Market and marketing analysis; d) Administration, finance and accounting; e) Intellectual protection and certification; f) Communication and relations with the territory; g) Access to public and private funds and funding; h) Community research policies; i) Community planning; l) Coordination, management and reporting of a community project; m) Organization of promotional events and territorial animation.

9) Role: Scientific Coordinator of the project

Project title:

- Conservation of the population of Calendula maritima Guss., a threatened species of Sicilian flora

- Salvaguardia delle popolazioni di Calendula maritima Guss., specie minacciata della flora siciliana

Type / Financing: Institut Klorane, Fondation d’Entreprise pour la Protection et la Bonne Utilisation du Patrimoine Végétal (Laboratoires Pierre Fabre) - Castres, France (http://www.institut-klorane.org/flash/fr/) - Convenzione Institut Klorane/IGV-CNR Prot. 0000757 IGV-CNR

Total amount of the Project (€): 40.000.00

Financing Amount for IGV-CNR Unit (€): 36.000.00

Other Italian or foreign partners: Institut Klorane, Fondation d’Entreprise pour la Protection et la Bonne Utilisation du Patrimoine Végétal (Laboratoires Pierre Fabre) - Castres, France; Istituto Agrario “Abele Damiani” di Marsala; Conservatoire Botanique National de Brest, France (CBNB); the Group of Plant Specialists of the Mediterranean Islands of the CSE - International Union for Conservation of Nature; il Dipartimento di Biologia Ambientale e Biodiversità dell’Università di Palermo (DBABUP)

Activity period: From: 27 February 2012 - To: 26 February 2014

Project Aims: Calendula maritima is an endemic species of Sicily particularly threatened, whose populations colonize some stretches of the coast of the Province of Trapani. In order to contribute to the conservation of this precious element of the island’s biological heritage and its habitat, a project was developed to protect the species and populations most at risk. The project has a duration of two years and is carried out by a partnership that includes the Conservatoire Botanique National de Brest, France (CBNB), the Group of Plant Specialists of the Mediterranean Islands (CSE / IUCN), the Department of Environmental Biology and Biodiversity of the University of Palermo (DBABUP), the IBBR-CNR), the Agricultural Institute ‘Abele Damiani’ of Marsala and the Regional Province of Trapani (PRTP).

Activity carried out: Three types of targeted and complementary actions were carried out within the project. The first two aim to ensure the ex situ conservation (action 1) and in situ (action 2) of the genetic diversity of the Calendula populations, through the realization of a seed bank representative of its natural biological diversity, the realization of a nursery of conservation, the strengthening of threatened populations, the implementation of restoration actions and management of degraded habitats in which the last populations exist. The third action (action 3) is aimed at raising awareness and consists of education and information activities that will allow to enhance the actions carried out in the field and to sensitize the local population on the issues of biodiversity conservation in general. The IBBR-CNR coordinated the actions carried out in Sicily.

Results achieved: The objectives foreseen by the project have been achieved. In fact, the condition of Calendula maritima is known in the territory of Trapani and new individuals have been successfully introduced in different areas of the coast of Trapani.

Web Site: http://www.institut-klorane.co.uk/saving-calendula-maritima

10) Role: Scientific Coordinator of the IGV-CNR Unit

Project title:

- CISIA: Innovative methods for the Made in Italy Agri-food industry. Sub-project: OR2.1.2 - Advanced tools for the genetic traceability of agro-food productions

- CISIA: Metodologie innovative per il Made in Italy Agroalimentare. Sottoprogetto: OR2.1.2 - Strumenti avanzati per la tracciabilità genetica delle produzioni agroalimentari

Type / Financing: Ministry of Economy and Finance, Italy, "CISIA" law 191/2009

Total amount of the Project (€): 437.000.00

Financing Amount for IGV-CNR Unit (€): 80.000.00

Other Italian or foreign partners: CNR Institutes: ISPA; IGV; IPP; ISAFOM; ISPAAM; ISA; IVV; IBBA; IBIMET; IBAF; IBP; IGB; ICB; ITB. Other partners: DIBCA-UNIBA, DEZA-UNINA, ARBOPAVE-UNINA, DISPA-UNICT, IGA-UNIUD, DCA-UNITO, DSA-UNIMI, CRA-FG e CRA-AR. Società: Metapontum Agrobios, Barilla, Porto Conte Ricerche, Advanced Agroindustry Technology, Eureco, Agritest, Bionat Italia, EnBiotech, Molino Casillo, Molino Tandoi-Pellegrino e Marchesi Antinori. Consorzi: DOP Sardegna, Puglia Natura

Activity period: From: 1 July 2011 - To: 30 June 2014

Project Aims: The project plans to develop an advanced molecular diagnostic network aimed at ensuring the quality and safety of Agri-food productions. The genetic-molecular characterization of some of the organisms that contribute is then implemented at the base of these products, in order to improve production and enhance its use in relation to the production chain and the respective territory. Finally, new products / technologies will be identified for the agri-food industry, also in response to the growing demand for foods with a high health content.

11) Role: Scientific Coordinator of the IGV-CNR Unit

Project title:

- Biological processes and environmental factors involved in the control of vegetative growth, fructification and the quality of olive oil (Olea europaea L.) in super-intensive plants. Subproject: In vitro study of the architecture of the foliage and of the root system of olive cultivars (Olea europaea L.) with different potential for vegetative growth

- Processi biologici e fattori ambientali coinvolti nel controllo della crescita vegetativa, della fruttificazione e della qualità dell’olio nell’olivo (Olea europaea L.) in impianti superintensivi. Sottoprogetto: Studio in vitro dell’architettura della chioma e del sistema radicale di cultivar di olivo (Olea europaea L.) con diverso potenziale di crescita vegetativa

Type / Financing: Ministry of Education, University and Research (MIUR) - Research Projects of National Interest (PRIN) 2008

Total amount of the Project (€): 102.000.00

Financing Amount for IGV-CNR Unit (€): 25.000.00

Other Italian or foreign partners: University of Palermo, University of Perugia; University of Naples, University of Bari

Activity period: From: 22 March 2010 - To: 21 March 2012

Project Aims: The traditional Italian olive farms, characterized by high production costs, are rather weak and are also supported by the European Union’s aid policy. In order to make the Italian olive oil sector more competitive in Europe and worldwide, it is necessary to look at the new, more modern, dynamic, highly productive and easily mechanized cultivation systems, such as intensive and super-intensive plants currently very widespread in Spain. The aim of the project is the in vitro study of the architecture of the root system and of the foliage in different olive cultivars with different potential for vegetative growth. Two cultivars typical of Italian areas will be compared in vitro with two low vigour Spanish cultivars, used in super-intensive systems. The study will allow to identify a possible in vitro screening protocol of clones with characters useful for the introduction of Italian cultivars, of proven qualitative value, in super intensive cultivation plants.

Activity carried out: The architecture of the root system and the foliage in various olive cultivars with different potential for vegetative growth in vitro has been studied. Two cultivars typical of Sicilian areas, Cerasuola (high vigour) and Biancolilla (medium vigour), were compared in vitro with two Spanish cultivars (Arbequina and Arbosana) and one Greek (Koroneiki) with low vigour, used in super-intensive systems. The study made it possible to identify an in vitro clone screening protocol with characters useful for the introduction of Italian cultivars, of proven qualitative value, in super-intensive cultivation plants.

12) Role: Scientific Coordinator of the IGV-CNR Unit

Project title:

- Bio.Me.P. - Design and Development of a Biosensor for the detection in "real-time" of Heavy Metals

- Bio.Me.P. - Progetto e Sviluppo di un Biosensore per le rilevazione in "real-time" di Metalli Pesanti

Type / Financing: European Structural Funds - European Regional Development Fund (ERDF) (PO FESR 2007/2013, Action 4.1.1.2); Decree granting funding, Sicilian Region, Regional Department of Productive Activities, Prot. 5747/3, 13 December 2011

Total amount of the Project (€): 563.475.59

Financing Amount for IGV-CNR Unit (€): 77.545.00

Other Italian or foreign partners: BioDiagene, IAMC-CNR, IBIM-CNR

Activity period: From: 8 February 2012 - To: 7 February 2014

Project Aims: The project is based on results obtained from the previous research "Identification and characterization of fluorescent proteins with different emission spectrum from marine organisms and applications in the environmental field", funded by the CNR. The project aims to study the effect of environmental contaminants on the fluorescent emission intensity of the "Green Fluorescent Protein (GFP)" protein. This fluorescent protein, which has already been extracted from marine organisms (Aequorea coerulescens and Anemonia sulcata) and on which the first results have been obtained, will be synthesized in the laboratory so that it can be subsequently produced in medium quantities at reduced prices. The capacity of this protein will then be exploited for the design and development of a biosensor for "real-time" detection of heavy metals. In particular, the interaction characteristics between the protein and the metals will be exploited, which show a "quenching" (i.e. a reduction of the fluorescence emitted by the protein) proportional to the amount of metal present in the solution under test. Particularly interesting results have been obtained in the interaction with copper which, among other things, represents one of the most pollutants present in agriculture and that, even today, it is not possible to completely eliminate the treatment of some biological crops (such as the grapevine).

Activity carried out: A miniaturized optoelectronic system designed for fluorescence reading has been designed that, in particular, serves to stimulate (through low power LEDs) and measure (through photodiodes) the ability to emit light of the samples under examination at different lengths wave, depending on the type of analysis to be performed. The final system was finally validated through the measurement of samples obtained through hydroponic and off-soil cultivation.

Results obtained: Design and development of a portable and automatic instrument for detection in "real-time" of heavy metals. At the end of the project BioDiagene obtained the prototype of a unique instrument at an international level, able to perform, in real time and with low costs of use, analytical investigations for the estimation of the presence of heavy metals in the samples under examination.

13) Role: Scientific Coordinator of the project

Project title:

- V.C.S. Virus Capture System

Type / Financing: European Structural Funds - European Regional Development Fund (ERDF) (PO FESR 2007/2013, Action 4.1.1.1); Decree granting funding, Sicilian Region, Regional Department of Productive Activities, Prot. 2254/3, 23 October 2013

Total amount of the Project (€): 1.288.500.00

Financing Amount for IGV-CNR Unit (€): 625.200.00

Other Italian or foreign partners: Bionat Italia Spa, Azienda Agricola Misita, Vivai Mannone

Activity period: From: 30 October 2013 - To: 31 December 2015

Project Aims: Virus Capture System is a project that has led to the development of a new rapid system for the early diagnosis of pathogens present in grapevine plants. The system, based on biomolecular technologies (LAMP PCR), is capable of transmitting data outcomes, resulting from the analysis, to a remote system which feeds a georeferenced database that is used for the implementation of an agricultural decision support system. The system is simple to use and therefore can be handled by unskilled personnel while working on open fields. The results of the project are composed of a set of technologies and best practices that have been applied to the wine industry, and can be applied in all agri-food sectors, for the benefit of food security and the implementation of a global system for early detection, monitoring and predictive test surveys regarding the transmission of plant diseases.

Results obtained: Development of diagnostic kits for pathogens in plant material using LAMP/PCR technology.

14) Role: Scientific Coordinator of the project

Project title:

- Tools and technologies for the typing and characterization of oils from Sicilian olives

- Strumenti e tecnologie per la tipicizzazione e la caratterizzazione degli oli da olive siciliane

Type / Financing: European Structural Funds - European Regional Development Fund (ERDF) (PO FESR 2007/2013, Action 4.1.1.2); Decree granting funding, Sicilian Region, Regional Department of Productive Activities, Prot. 5750, 13 December 2011

Total amount of the Project (€): 657.150.00

Financing Amount for IGV-CNR Unit (€): 288.210.00

Other Italian or foreign partners: Bionat Italia

Activity period: From: 2 February 2012 - To: 1 February 2014

Project Aims: The aim of the project is the development of a system of genetic traceability of products of the olive industry (olives and extra virgin olive oil) that can be used by operators and visible to consumers. In particular, it aims to create a system that, through the application of molecular genetic technologies, allows recognizing the origin of the olives used in the production of commercial olive oil. Activities carried out: Methods have been developed to identify the cultivars used for the production of finished products. Within the project DNA-based markers such as chloroplast microsatellites (SSRcp) and SNP were tested and developed, whose analysis has the advantage of being associated with the PCR method, which allows amplifying small quantities of starting DNA as in the case of oils. The sequences identified were used to design specific primers to be used in Real Time PCR platforms, in order to analyze the DNA of Sicilian varieties and compare them with the profiles obtained from the autochthonous cultivar standards present in the CNR germplasm collection, to ensure and certify the authenticity of the finished product. The implications of the project results are of great importance in terms of prevention and detection of fraud, because it will be possible to verify compliance with the regulations or the presence in the finished product of genetic material that differs from that declared by the manufacturer, in a historical period in which the economic crisis is causing enormous difficulties in enhancing and spreading local productions at the expense of the cheapest but of dubious origin and quality. Results obtained during the project: 1) an analytical and methodological protocol for the identification of the varietal composition and the certification of typicality of extra virgin olive oil were designed, developed and validated; 2) an automatic analytical tool able to apply and make use of the protocol and the methodologies developed by non-specialized operators.

15) Role: Scientific Coordinator of the project

Project title:

- Genetic and sanitary improvement of traditional Sicilian grapevine cultivars

- Recupero e valorizzazione dei vitigni tradizionali siciliani

Type / Financing: European Structural Funds - European Regional Development Fund (ERDF) (PO FESR 2007/2013, Action 4.1.1.1); Decree granting funding, Sicilian Region, Regional Department of Productive Activities, Prot. 3408, 4 August 2011

Total amount of the Project (€): 1.004.938.42

Financing Amount for IGV-CNR Unit (€): 417.319.42

Other Italian or foreign partners: Bionat Italia, Vivai Mannone, Consorzio Isola Bio Sicilia, Cantine Bagliesi

Activity period: From: 3 October 2011 - To: 3 March 2015

Project Aims: The aim of the project is to identify, within the grapevine cultivated in Sicily, biotypes with qualitative characteristics and a high sanitary standard. The focus will be mainly on Catarratto, Grillo and some black varieties with high colouring power. Through clonal selection (constantly accompanied by genetic, biochemical and sanitary analyses) new and innovative plant material will be supplied to the wine-growing and nursery companies, safe from a genetic and health point of view, with characteristics that respond to market needs, such as for example, as regards white wines, clones with low concentrations of catechins (easily oxidizable polyphenolic substances), which will allow at the moment of bottling a reduction of browning risks and a greater "shelf-life" of the wine, a problem particularly felt for some cultivars such as the Catarratto. Parallel to the clonal selection, a population of Catarratto mutants will be established, using in vitro mutational breeding, characterized by a short internode and therefore by a reduced development, to rationalize the execution of cultivation techniques. For this purpose, in vitro regenerated somatic embryos are subjected to mutagenic treatment, at the end of which the mutagenized individuals will be selected. Mutagenized plants are transferred to the open field for further morphological and physiological observations. Finally, for the viruses elimination from the most interesting clones of Catarratto and Grillo, somatic embryogenesis will be used, an innovative in vitro rehabilitation technique that eliminates many viruses with high efficiency.

Activity carried out: Clonal selection activities were carried out in the main wine-growing areas of the region. From the most interesting selected clones, embryogenic lines were regenerated; from these new plants have been regenerated in vitro that have been subjected to sanitary analyses to verify their health status. Furthermore, new mutated clones were produced by subjecting the embryogenic cell lines to treatment with mutagens.

Results obtained: Genetic improvement of some native Sicilian grapevines, using classical and innovative techniques (clonal selection, somatic embryogenesis and mutagenesis). In particular, new clones of traditional Sicilian grapevines with important "commercial" characteristics have been regenerated in vitro after removal of viruses.

16) Role: Scientific Coordinator of the project

Project title:

- Genetic certification of Sicilian wine

- Certificazione Genetica del vino di Origine Siciliana

Type / Financing: Rural development programme for Sicilian Region, Department of Agricultural Resources and Food, Action 1.2.4, Decree of funding, Prot. 1362, 11 October 2011

Total amount of the Project (€): 965.664.25

Financing Amount for IGV-CNR Unit (€): 173.833.42

Other Italian or foreign partners: Azienda Vinicola Benanti, Aziende Agricole Planeta, Cantine Settesoli, Azienda Agricola Bonivini, Vivaio Luigi Saladino, Cantina Sociale Primavera, Cantine Siciliane Riunite, Graham e Associati

Activity period: From: 17 November 2011 - To: 17 May 2014

Project Aims: The purpose of the project is to achieve a new certification of the wine industry based on innovative genetic and biogeochemical methods. This new certification allows identifying, in a precise and scientific way, the product (genetic method) and the territory of origin (biogeochemical method). At the end of the project some Sicilian products (selected as pilot) will be certified with the new certification of genetic and biogeochemical quality. In particular, the leading company (Bionat Italia) intends to set up a laboratory for the certification of typicality and provenance of wines, offering services to companies in the wine industry.

Activities: The 17 Sicilian grapevine cultivars and the main clones present in the typical production areas were collected and genetically and sanitary characterized. DNA has been extracted from the various products of the wine chain, and amplified with molecular markers for genetic traceability. In particular, molecular markers were identified for the recognition of different varieties. Protocols for the traceability of wines have been developed.

Results obtained: Development of molecular genetic traceability technologies for the characterization of products of the wine chain (grafted grafts, grapes, must and wine), which allows to recognize / discriminate the products of vines during the different production phases.

17) Role: Scientific Coordinator of the IGV-CNR Unit

Project title:

- Comparison of new citrus varieties for the extension of the harvesting period and the production of fresh juices

- Introduzione di nuove varietà di agrumi destinate alla diversificazione del periodo di raccolta ed alla produzione di succhi freschi

Type / Financing: Rural development programme for Sicilian Region, Department of Agricultural Resources and Food, Action 1.2.4, Decree of funding, Prot. 1048, 22 August 2011

Total amount of the Project (€): 427.000.00

Financing Amount for IGV-CNR Unit (€): 69.000.00

Other Italian or foreign partners: Agroindustry Advanced Technologies S.p.a., ISAFOM-CNR, Citrus growers

Activity period: From: 26 September 2011 - To: 25 September 2013

Project Aims: The project aims to transfer the knowledge acquired by CNR, to transfer innovation with the aim of increasing the competitiveness of companies that want to use new varieties / citrus clones to expand the fruit harvesting calendar.

Activity carried out: The studies and researches supported are aimed at enhancing and improving the quality of citrus production with a view to increasing the competitiveness of companies in the sector. The qualitative improvement and the valorization of the product are obtained also with the increase of the varietal panorama and the extension of the cultivation and harvesting period. The application phase of these researches, aimed at introducing new varieties, leads to the strengthening of competitiveness within a market that must withstand the impact of the globalization of markets for marketing, even in non-EU areas. In this context, the cultivation of early and late citrus varieties will increase the commercial outlets occupying market niches with more profitable prices.

Results obtained: As part of the project, new clones / varieties, created by the CNR, were identified, capable of extending the collection calendar. New genotypes suitable for both traditional fresh consumption and new squeezing systems have been identified.

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Publications ISI/SCOPUS

  1. Pathirana R, Blouin A, Hedderley D, McLachlan A, Panis B, Carimi F (2018) Cryotherapy using droplet vitrification eliminates Three Leafroll Viruses from infected grapevine (Vitis vinifera L.) varieties. Submitted;
  2. Mercati F, Fontana I, Martorana A, El Nagar M, Gristina AS, Carimi F (2018) De novo transcriptome assembly and new molecular markers discovery in Capparis spinosa L. Submitted;
  3. Benalia H, Gristina AS, Elkader SA, Mercati F, Nassima H, Martorana A, Sharaf A, Carimi F (2018) Nuclear and chloroplast microsatellites highlight an exclusive genetic core among the main Algerian olive cultivars. Submitted;
  4. Selmi I, Pacifico D, Lehad A, Carimi F, Mahfoudhi N (2018) Genetic diversity of Grapevine rupestris stem pitting associated virus isolates from spontaneous and cultivated grapevines. Submitted;
  5. Faddetta T, Abbate L, Renzone G, Palumbo Piccionello A, Maggio A, Oddo E, Scaloni A, Puglia AM, Gallo G, Carimi F, Fatta Del Bosco S, Mercati F (2018) An integrated proteomic and metabolomic study to evaluate the effect of nucleus-cytoplasm interaction in a diploid citrus cybrid between sweet orange and lemon. Plant Molecular Biology. 98:407-425, DOI: 10.1007/s11103-018-0787-9;
  6. Selmi I, Lehad A, Pacifico D, Carimi F, Mahfoudhi N (2018) Prevalence and genetic diversity of Grapevine virus A isolates in Tunisia. Phytopathologia Mediterranea. 57(2), 237-244, DOI: 10.14601/Phytopathol_Mediterr-22123;
  7. Allegra A, Gallotta A, Carimi F, Mercati F, Inglese P, Martinelli F (2018) Metabolic profiling and post-harvest behavior of ‘Dottato’ fig (Ficus carica L.) fruit covered with an edible coating from O. ficus-indica. Frontiers in Plant Science. 9:1321, DOI: 10.3389/fpls.2018.01321, WOS:000443722400001;
  8. Zito P, Tavella F, Sajeva M, Carimi F, Dötterl S (2018) Inflorescence scents of Calendula maritima, C. suffruticosa subsp. fulgida, and their hybrid. International Journal of Plant Sciences. 179(5):415-421, DOI: 10.1086/697240, WOS:000433464300007;
  9. Polat I, Baysal O, Mercati F, Gümrükcü E, Sülü G, Kitapcı A, Devran Z, Carimi F (2018) Characterization of Botrytis cinerea isolates collected on pepper in Southern Turkey by using molecular markers, fungicide resistance genes and virulence assay. Infection, Genetics and Evolution. 60:151-159, DOI 10.1016/j.meegid.2018.02.019, WOS:000428317200021;
  10. Zito P, Serraino F, Carimi F, Tavella F, Sajeva M (2018) Inflorescence-visiting insects of a functionally dioecious wild grapevine. Genetic Resources and Crop Evolution. 65(5):1329-1335, DOI 10.1007/s10722-018-0616-7, WOS:000431788800002;
  11. Raji MR, Lotfi M, Tohidfar M, Zahedi B, Carra A, Abbate L, Carimi F (2018) Somatic embryogenesis of muskmelon (Cucumis melo L.) and genetic stability assessment of regenerants using flow cytometry and ISSR markers. Protoplasma. 255(3):873-883, DOI 10.1007/s00709-017-1194-9, WOS:000430302400015;
  12. Haddad B, Carra A, Saadi A, Haddad N, Mercati F, Gristina AS, Boukhalfa S, Djillali A, Carimi F (2018) In vitro propagation of the relict Laperrine’s olive (Olea europaea L. subsp. laperrinei). Plant Biosystems. 152(4):621-630, DOI 10.1080/11263504.2017.1306002, WOS:000432652900006;
  13. Selmi I, Pacifico D, Harbi Ben Slimane M, Garfi G, Carimi F, Mahfoudhi N (2017) First report of grapevine Rupestris stem pitting-associated virus in wild grapevines (Vitis vinifera spp. sylvestris) in Tunisia. Plant Disease. 102(2):458, DOI 10.1094/PDIS-05-17-0780-PDN, WOS:000423496600076;
  14. Bouamama-Gzara B, Selmi I, Chebil S, Melki I, Mliki A, Ghorbel A, Carra A, Carimi F, Mahfoudhi N (2017) Elimination of Grapevine leafroll associated virus-3, Grapevine rupestris stem pitting associated virus and Grapevine virus A from a Tunisian cultivar by somatic embryogenesis and characterization of the somaclones using ampelographic descriptors. The Plant Pathology Journal. 33:561-571, DOI 10.5423/PPJ.OA.06.2017.0122, WOS:000417010100004;
  15. Selmi I, Lehad A, Pacifico D, Carimi F, Mahfoudhi N (2017) First report of Grapevine Virus E and Grapevine Virus F in Tunisian grapevines. Journal of Plant Pathology. 99(2):543, DOI 10.4454/jpp.v99i2.3904, WOS:000410696900053;
  16. Selmi I, Pacifico D, Lehad A, Garfi G, Carimi F, Mahfoudhi N (2017) Occurrence of grapevine virus A in wild grapevines (Vitis vinifera subsp. sylvestris) in Tunisia. Journal of Plant Pathology. 99(3):799-817, DOI 10.4454/jpp.v99i3.3983, WOS:000418566300068;
  17. Meziane M, Frasheri D, Carra A, Boudjeniba M, D’Onghia AM, Mercati F, Djelouah K, Carimi F (2017) Attempts to eradicate graft-transmissible infections through somatic embryogenesis in Citrus ssp. and analysis of genetic stability of regenerated plants. European Journal of Plant Pathology. 148(1):85-95, DOI: 10.1007/s10658-016-1072-x, WOS:000399024900009;
  18. Gristina AS, De Michele R, Garfì G, La Mantia T, Fontana I, Spinelli P, Motisi A, Carimi F (2017) Urgent need for preservation of grapevine (Vitis vinifera L. Subsp. vinifera) germplasm from small circum-Sicilian islands as revealed by SSR markers and traditional use investigations. Genetic Resources and Crop Evolution. 64(6):1395-1415, DOI: 10.1007/s10722-016-0444-6, WOS:000404901300021;
  19. Fatta Del Bosco S, Napoli E, Mercati F, Abbate L, Carimi F, Ruberto G (2017) Somatic cybridization for Citrus: polyphenols distribution in juices and peel essential oil composition of a diploid cybrid from Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and sour orange (Citrus aurantium L.). Genetic Resources and Crop Evolution. 64(2):261-275, DOI: 10.1007/s10722-015-0348-x, WOS:000394345800003;
  20. Pasta S, Garfì G, Carimi F, Marcenò C (2017) Human disturbance, habitat degradation and niche shift: the case of the endemic Calendula maritima Guss. (W Sicily, Italy). Rendiconti Lincei. 28(2):415-424, DOI: 10.1007/s12210-017-0611-5, WOS:000401766800014;
  21. Pacifico D, Crucitti D, Stigliano E, Ciuffo M, Vallino M, Carimi F (2016) First Report of Colombian datura virus in Mandragora autumnalis in Sicily, Italy. Plant Disease. 100(11):2338-2339, DOI: 10.1094/PDIS-04-16-0445-PDN;
  22. Carimi F, Carra A, Panis B, Pathirana R (2016) Strategies for conservation of endangered wild grapevine (Vitis vinifera L. subsp. sylvestris (C.C. Gmel.) Hegi). Acta Horticulturae. 1115:81-86, DOI: 10.17660/ActaHortic.2016.1115.13;
  23. Carra A, Sajeva M, Abbate L, Siragusa M, Pathirana R, Carimi F (2016) Factors affecting somatic embryogenesis in eight Italian grapevine cultivars and the genetic stability of embryo-derived regenerants as assessed by molecular markers. Scientia Horticulturae. 204:123-127, DOI: 10.1016/j.scienta.2016.03.045;
  24. Sommella E, Pepe G, Pagano F, Conte G, Carimi F, Tenore GC, Novellino E, Manfra M, Russo M, Campiglia P (2016) Rapid screening of antioxidant anthocyanins in autochthonous Nero d’Avola grape clones by pre-column DPPH reaction coupled to UHPLC-UV/Vis-IT-TOF: a strategy to combine chemical data and genetic diversity. Food Analytical Methods. 9(10):2780-2790, DOI: 10.1007/s12161-016-0472-z;
  25. Zito P, Scrima A, Sajeva M, Carimi F, Dötterl S (2016) Dimorphism in inflorescence scent of dioecious wild grapevine. Biochemical Systematics and Ecology. 66:58-62, DOI: 10.1016/j.bse.2016.03.003;
  26. Carra A, Bambina M, Pasta A, Garfì G, Badalamenti O, Catalano C, Carimi F, Sajeva M (2016) In-vitro regeneration of Calendula maritima Guss. (Asteraceae), a threatened plant endemic to Western Sicily. Pakistan Journal of Botany. 48(2):589-593, ISSN: 05563321;
  27. Badalamenti O, Carra A, Oddo E, Carimi F, Sajeva M (2016) Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study. SpringerPlus. 5(201):1-4, DOI: 10.1186/s40064-016-1901-6;
  28. Pacifico D, Stigliano E, Sposito L, Spinelli P, Garfì G, Gristina AS, Fontana I, Carimi F (2016) Survey of viral infections in spontaneous grapevines from natural environments in Sicily. European Journal of Plant Pathology. 145(1):189-197, DOI 10.1007/s10658-015-0785-6;
  29. Pathirana R, McLachlan A, Hedderley D, Panis B, Carimi F (2016) Pre-treatment with salicylic acid improves plant regeneration after cryopreservation of grapevine (Vitis spp.) by droplet vitrification. Acta Physiologiae Plantarum. 38(1):1-12, DOI: 10.1007/s11738-015-2026-1;
  30. Ruisi P, Longo M, Martinelli F, Di Miceli G, Frenda AS, Saia S, Carimi F, Giambalvo D, Amato G (2015) Morpho-agronomic and genetic diversity among twelve Sicilian agro-ecotypes of lentil (Lens culinaris). The Journal of Animal & Plant Sciences. 25(3):716-728, ISSN: 10187081;
  31. Pathirana R, McLachlan A, Hedderley D, Carra A, Carimi F, Panis B (2015) Removal of leafroll viruses from infected grapevine plants by droplet vitrification. Acta Horticulturae. 1083:491-498, ISBN: 978-946261077-4;
  32. De Michele R, Carimi F, Frommer WB (2014) Mitochondrial Biosensors. International Journal of Biochemistry & Cell Biology. 48(1):39-44, DOI: 10.1016/j.biocel.2013.12.014;
  33. Ruberti C, Barizza E, Bodner M, La Rocca N, De Michele R, Carimi F, Lo Schiavo F, Zottini M (2014) Mitochondria change dynamics and morphology during grapevine leaf senescence. PLoS One. 9(7): e102012, DOI: 10.1371/journal.pone.0102012;
  34. Gristina AS, Fici S, Siragusa M, Fontana I, Garfì G, Carimi F (2014) Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex. Flora-Morphology, Distribution, Functional Ecology of Plants. 209(12):733-741, DOI: 10.1016/j.flora.2014.09.002;
  35. Polat I, Baysal O, Mercati F, Kitner M, Cohen Y, Lebeda A, Carimi F (2014) Characterization of Pseudoperonospora cubensis isolates from Europe and Asia using ISSR and SRAP molecular markers. European Journal of Plant Pathology. 139(3):641-653, DOI: 10.1007/s10658-014-0420-y;
  36. Baysal Ö, Lai D, H-H Xu, Siragusa M, Çalışkan M, Carimi F, Teixeria da Silva JA, Tör M (2013) A proteomic approach provides new insights into the control of soil-borne plant pathogens by Bacillus species. PLoS One. 8(1):e53182, DOI: 10.1371/journal.pone.0053182;
  37. Garfì G, Mercati F, Fontana I, Collesano G, Pasta S, Vendramin GG, De Michele R, Carimi F (2013) Habitat features and genetic integrity of wild grapevine Vitis vinifera L. subsp. sylvestris (C.C. Gmel.) Hegi populations: a case study from Sicily. Flora-Morphology, Distribution, Functional Ecology of Plants. 208(8-9):538-548, DOI: 10.1016/j.flora.2013.08.005;
  38. Baysal Ö, Karaaslan Ç, Siragusa M, Allesandro R, Carimi F, De Pasquale F, Teixeira Da Silva J (2013) Molecular markers reflect differentiation of Fusarium oxysporum forma speciales on tomato and forma on eggplant. Biochemical Systematics and Ecology. 47:139-147, DOI: 10.1016/j.bse.2012.10.005;
  39. Carra A, Sajeva M, Abbate L, Siragusa M, Sottile F, Carimi F (2012) In vitro plant regeneration of caper (Capparis spinosa L.) from floral explants and genetic stability of regenerants. Plant Cell, Tissue and Organ Culture. 109(2):373-381, DOI: 10.1007/s11240-011-0102-9;
  40. Fiore MC, Carimi F, Carra A, Sunseri F (2012) Efficient plant regeneration via somatic embryogenesis in bulbing fennel using immature flowers explants. In Vitro Cellular and Developmental Biology - Plant. 48(5):440-445, DOI: 10.1007/s11627-012-9441-4;
  41. Meziane M, Boudjeniba M, Frasheri D, D’Onghia AM, Carra A, Carimi F, Haddad N, Boukhalfa S, Braneci S (2012) Regeneration of Algerian Citrus germplasm by stigma/style somatic embryogenesis. African Journal of Biotechnology. 11(25):6666-6672, DOI: 10.5897/AJB10.2485;
  42. Carra A, Del Signore MB, Sottile F, Ricci A, Carimi F (2012) Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L. Plant Growth Regulation. 66(2):229-237, DOI: 10.1007/s10725-011-9645-3;
  43. Meziane M, Boudjeniba M, Frasheri D, D’Onghia AM, Carra A, Carimi F (2012). A first attempt on sanitation of Algerian Citrus genotypes by stigma/ style somatic embryogenesis. Acta Horticulturae. 940:713-718, ISBN: 978-906605685-5;
  44. Carimi F, Mercati F, De Michele R, Fiore MC, Riccardi P, Sunseri F (2011) Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d’Avola’ as revealed by microsatellite markers. Genetic Resources Crop Evolution. 58(7):967-975, DOI: 10.1007/s10722-011-9731-4;
  45. Garfì G, Carimi F, Pasta S, Rüh J, Trigila S (2011) Additional insights on the ecology of the relic tree Zelkova sicula Di Pasquale, Garfì et Quézel (Ulmaceae) after the finding of a new population. Flora-Morphology, Distribution, Functional Ecology of Plants. 206(5):407-417, DOI: 10.1016/j.flora.2010.11.004;
  46. Baysal O, Mercati F, Ikten H, Yildiz RC, Carimi F, Aysan Y, Teixeira da Silva JA (2011) Clavibacter michiganensis subsp. michiganesis: tracking strains using their genetic differentiations by ISSR markers in Southern Turkey. Physiological and Molecular Plant Pathology. 75(3):113-119, DOI: 10.1016/j.pmpp.2010.10.002;
  47. La Mantia T, Carimi F, Di Lorenzo R, Pasta S (2011) The agricultural heritage of Lampedusa (Pelagie Archipelago, South Italy) and its key role for cultivar and wildlife conservation. Italian Journal of Agronomy. 6(2):106-110, DOI: 10.4081/ija.2011.e17;
  48. Ruisi P, Siragusa M, Di Giorgio G, Graziano D, Amato G, Carimi F, Giambalvo D (2011) Pheno-morphological, agronomic and genetic diversity among natural populations of sulla (Hedysarum coronarium L.) collected in Sicily, Italy. Genetic Resources Crop Evolution. 58(2):245-257, DOI: 10.1007/s10722-010-9565-5;
  49. Baysal O, Siragusa M, Gümrükcü E, Zengin S, Carimi F, Sajeva M, Teixeira da Silva JA (2010) Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant. Biochemical Genetics. 48(5-6):524-537, DOI: 10.1007/s10528-010-9336-1;
  50. Carimi F, Mercati F, Abbate L, Sunseri F (2010) Microsatellite analyses for evaluation of genetic diversity among Sicilian grapevine cultivars. Genetic Resources Crop Evolution. 57(5):703-719, DOI: 10.1007/s10722-009-9506-3;
  51. De Michele R, Formentin E, Todesco M, Toppo S, Carimi F, Zottini M, Barizza E, Ferrarini A, Delledonne M, Fontana P, Lo Schiavo F (2009) Transcriptome analysis of Medicago truncatula leaf senescence: similarities and differences in metabolic and transcriptional regulations as compared with Arabidopsis, nodule senescence and nitric oxide signalling. New Phytologist. 181(3):563-575, DOI: 10.1111/j.1469-8137.2008.02684.x;
  52. Baysal O, Siragusa M, Ikten H, Polat I, Gümrükcü E, Yigit F, Carimi F, Teixeira da Silva JA (2009) Fusarium oxysporum f. sp. lycopersici races and their genetic discrimination by molecular markers in West Mediterranean region of Turkey. Physiological and Molecular Plant Pathology. 74(1):68-75, DOI: 10.1016/j.pmpp.2009.09.008;
  53. Siragusa M, Carimi F (2009) Development of specific primers for cpSSR analysis in caper, olive and grapevine using consensus chloroplast primer pairs. Scientia Horticulturae. 120(1):14-21, DOI: 10.1016/j.scienta.2008.09.002;
  54. Pathirana R, Cheah LH, Carimi F, Carra A (2009) Low temperature stored Botrytis cinerea in cryobank maintains pathogenicity in grapevine. Cryoletters. 30(1):84-84, ISSN: 0143-2044;
  55. Zottini M, Barizza E, Costa A, Formentin E, Ruberti C, Carimi F, Lo Schiavo F (2008) Agroinfiltration of grapevine leaves for fast transient assays of gene expression and for long-term production of stable transformed cells. Plant Cell Reports. 27(5):845-853, DOI: 10.1007/s00299-008-0510-4;
  56. Sajeva M, Carra A, De Pasquale F, Carimi F (2008) Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon). Plant Biosystems. 142(2):199-203, DOI: 10.1080/11263500802150258;
  57. Zottini M, Costa A, De Michele R, Ruzzene M, Carimi F, Lo Schiavo F (2007) Salicylic acid activates nitric oxide synthesis in Arabidopsis. Journal of Experimental Botany. 58(6):1397-1405, DOI: 10.1093/jxb/erm001;
  58. Siragusa M, Carra A, Salvia L, Puglia AM, De Pasquale F, Carimi F (2007) Genetic instability in calamondin (Citrus madurensis Lour.) plants derived from somatic embryogenesis induced by diphenylurea derivatives. Plant Cell Reports. 26(8):1289-1296, DOI: 10.1007/s00299-007-0326-7;
  59. Zottini M, Barizza E, Bastianelli F, Carimi F, Lo Schiavo F (2006) Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondria morphology. New Phytologist. 172(2):239-247, DOI: 10.1111/j.1469-8137.2006.01830.x;
  60. Carra A, De Pasquale F, Ricci A, Carimi F (2006) Diphenylurea derivatives induce somatic embryogenesis in Citrus. Plant Cell, Tissue and Organ Culture. 87(1):41-48, DOI: 10.1007/s11240-006-9132-0;
  61. Carimi F, Zottini M, Costa A, Cattelan I, De Michele R, Terzi M, Lo Schiavo F (2005) NO signaling in cytokinin-induced programmed cell death. Plant Cell Environment. 28(9):1171-1178, DOI: 10.1111/j.1365-3040.2005.01355.x;
  62. Carimi F, Barizza E, Gardiman G, Lo Schiavo F (2005) Somatic embryogenesis from stigmas and styles of grapevine. In Vitro Cellular and Developmental Biology - Plant. 41(3):249-252, DOI: 10.1079/IVP2004617;
  63. Zottini M, Terzi M, De Michele R, Lo Schiavo F, Carimi F (2005) NO signaling and cytokinins. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 141:238, doi:10.1016/j.cbpb.2005.05.019;
  64. Carimi F, Terzi M, De Michele R, Zottini M, Lo Schiavo F (2004) High levels of the cytokinin BAP induce PCD by accelerating senescence. Plant Science. 166(4):963-969, DOI: 10.1016/j.plantsci.2003.12.016;
  65. Morgana C, Di Lorenzo R, Carimi F (2004) Somatic embryogenesis of Vitis vinifera L. (cv. Sugraone) from stigma and style culture. Vitis. 43(4):169-173, ISSN: 00427500;
  66. Carimi F, Zottini M, Formentin E, Terzi M, Lo Schiavo F (2003) Cytokinins: new apoptotic inducers in plants. Planta. 216(3):413-21, ISSN: 0032-0935;
  67. Quatrini P, Gentile M, Carimi F, De Pasquale F, Puglia AM (2003) Effect of native arbuscular mycorrhizal fungi and Glomus mosseae on acclimatisation and development of micropropagated Citrus limon (L.) Burm. Journal of Horticultural Science & Biotechnology. 78(1):39-45, DOI: 10.1080/14620316.2003.11511584;
  68. Zottini M, Formentin E, Scattolin M, Carimi F, Lo Schiavo F, Terzi M (2002) Nitric oxide affects plant mitochondrial functionality in vivo. FEBS Letters. 515(1-3):75-78, DOI: 10.1016/S0014-5793(02)02438-9;
  69. Fiore S, De Pasquale F, Carimi F, Sajeva M (2002) Effect of 2.4-D and 4-CPPU on somatic embryogenesis from stigma and style transverse thin layers of Citrus. Plant Cell, Tissue and Organ Culture. 68(1):57-63, DOI: 10.1023/A:1012944100210;
  70. D’Onghia AM, Carimi F, De Pasquale F, Djelouah K, Martelli GP (2001) Elimination of citrus psorosis virus by somatic embryogenesis from stigma and style cultures. Plant Pathology. 50(2):266-269, DOI: 10.1046/j.1365-3059.2001.00550.x;
  71. Carimi F, Caleca V, Mineo G, De Pasquale F, Crescimanno FG (2000) Rearing of Prays citri on callus derived from lemon stigma and style. Entomologia Experimentalis et Applicata. 95(3):251-257, DOI: 10.1023/A:1004020310036;
  72. Carimi F, De Pasquale F, Crescimanno FG (1999) Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Reports. 18(11):935-940, DOI: 10.1007/s002990050687;
  73. De Pasquale F, Giuffrida S, Carimi F (1999) Minigrafting of shoots, roots, inverted roots and somatic embryos for rescue of in vitro regenerants of Citrus. J American Society Horticultural Science. 124(2):152-157, ISSN: 00031062;
  74. Carimi F, Tortorici MC, De Pasquale F, Crescimanno FG (1998) Somatic embryogenesis and plant regeneration from undeveloped ovules and stigma/style explants of sweet orange navel group [Citrus sinensis (L.) Osb.]. Plant, Cell, Tissue and Organ Culture. 54(3):183-189, DOI: 10.1023/A:1006113731428;
  75. Carimi F, De Pasquale F, Puglia AM (1998) In vitro rescue of zygotic embryos of sour orange, Citrus aurantium L., and their detection based on RFLP analysis. Plant Breeding. 117(3):261-266, DOI: 10.1111/j.1439-0523.1998.tb01936.x;
  76. Carimi F, Di Lorenzo R, Crescimanno FG (1997) Callus induction and somatic embryogenesis in carob (Ceratonia siliqua L.) from ovule culture. Scientia Horticulturae. 70(1):73-79, DOI: 10.1016/S0304-4238(97)00034-4;
  77. D’Onghia AM, De Pasquale F, Carimi F, Savino V, Crescimanno FG (1997) Somatic embryogenesis from style culture as possible means for virus elimination in Citrus. Journal Phytopathology. 145(2-3):77-79, DOI: 10.1111/j.1439-0434.1997.tb00367.x;
  78. Carimi F, De Pasquale F, Crescimanno FG (1995) Somatic embryogenesis in Citrus from styles culture. Plant Science. 105(1):81-86, DOI: 10.1016/0168-9452(94)04032-C, WOS:A1995QK33900009;
  79. Sajeva M, Carimi F (1995) Micropropagation of cacti and other succulent plants. Plant Biosystems (Giornale Botanico Italiano). 129(2):60, DOI: 10.1080/11263509509431064;
  80. Sajeva M, Carimi F, Van Jaarsveld E (1995) In vitro propagation of Aloe polyphylla siionland ex pillans (Aloaceae). Plant Biosystems (Giornale Botanico Italiano). 129(2):61 DOI: 10.1080/11263509509431064;
  81. De Pasquale F, Carimi F, Crescimanno FG (1994) Somatic embryogenesis from styles of different cultivars of Citrus limon (L.) Burm. Australian Journal of Botany. 42(5):587-594, DOI: 10.1071/BT9940587, WOS:A1994PT25700008;
  82. Carimi F, De Pasquale F, Crescimanno FG (1994) Somatic embryogenesis from styles of lemon (Citrus limon). Plant, Cell, Tissue and Organ Culture. 37(2):209-211, DOI: 10.1007/BF00043619, WOS:A1994PT25700008;
  83. Sajeva M, Carimi F, Crescimanno FG (1994) In vitro propagation and rescue of a clone of Nolina recurvata (Lem.) Hemsley (Agavaceae). Plant Biosystems (Giornale Botanico Italiano). 128(1):266, DOI:10.1080/11263509409437107, WOS:A1994MQ60200009;
  84. Inglese P, Barbera G, Carimi F (1994) The effect of different amounts of cladode removal on reflowering of cactus pear (Opuntia ficus-indica (L.) Miller). Journal of Horticultural Science and Biotechnology. 69(1):61-65, DOI:10.1080/14620316.1994.11515249, WOS:A1994MQ60200009;
  85. Barbera G, Carimi F, Inglese P, Panno M (1992) Physical, morphological and chemical changes during fruit development and ripening in three cultivars of prickly pear Opuntia ficus-indica (L.) Miller. Journal of Horticultural Science and Biotechnology. 67(3):307-312, ISSN : 0022-1589; DOI:10.1080/00221589.1992.11516253, WOS:A1992HY12000003;
  86. Barbera G, Carimi F, Inglese P (1992) Past and present role of the indian-fig prickly pear (Opuntia ficus-indica (L.) Miller, Cactaceae) in the agriculture of Sicily. Economic Botany. 46(1):10-20, DOI:10.1007/BF02985249, WOS:A1992HM74700002;
  87. Barbera G, Carimi F, Leone F (1988) Effects of different levels of water stress on yield and quality of lemon trees. Citriculture. 1(4):717-722, ISBN:3-8236-1136-4, WOS:A1989BT66J00086.

Book Chapters

  1. Abbate L, Ben Hadj Alouane RB, Askri F, Ben Mohamed H, Bouamama B, Carimi F, et al. (2016) Carte génétique des principales vignes autochtones Siciliennes et Tunisiennes. Arti Grafiche Campo s.r.l. - Alcamo, pp. 58;
  2. Carimi F, D’Onghia AM, Carra A, Djelouah K (2013) Somatic embryogenesis and virus elimination in Citrus. In: Aslam J, Srivastava PS, Sharma MP (Eds.) Somatic Embryogenesis and Gene Expression. Narosa Publishing House, pp 124-145. ISBN: 9788184872279;
  3. Carimi F, Pathirana R, Carra A (2013) Somatic Embryogenesis and Agrobacterium Mediated Genetic Transformation in Vitis. In: Aslam J, Srivastava PS, Sharma MP (Eds.) Somatic Embryogenesis and Genetic Transformation in Plants. Narosa Publishing House, pp 179-218. ISBN 9788184872279;
  4. Gamboni M, Carimi F, Migliorini P (2012) Mediterranean diet: an integrated view. In: Sustainable Diets and Biodiversity - Direction and solutions for policy, research and action. Burlingame B and Dernini S (Ed.). FAO Publisher, pp 262-273. ISBN 9789251072882;
  5. Carimi F, Pathirana R, Carra A (2012) Biotechnologies for grapevine germplasm management and improvement. In: Grapevines: Varieties, Cultivation and Management, Petra V. Szabo and Jorge Shojania (Eds.). Nova Science Publishers, New York, 8:199-249. ISBN 9781621003618;
  6. Pasta S, Garfì G, La Bella F, Rühl J, Carimi F (2011) An overview on the human exploitation of Sicilian native edible plants. In: Wild Plants: Identification, Uses and Conservation, Ryan E. Davis (Ed.). Nova Science Publishers, New York. 6: 195-268. ISBN 9781612099668;
  7. Meziane M, Frasheri D, Carra A, Djelouah K, Carimi F, D’Onghia AM (2009) Citrus sanitation methods for the elimination of Citrus tristeza virus (CTV). In: D’Onghia AM, Djelouah K, Roistacher CN (Eds.) Citrus Tristeza Virus and Toxoptera citricidus: a serious threat to the Mediterranean citrus industry. Bari: CIHEAM-IAMB, Options Méditerranéennes: Série B. Etudes et Recherches, 65: 177-180. ISBN 2853524361;
  8. De Michele R, Zottini M, Lo Schiavo F, Carimi F (2006) Cross Talk between Nitric Oxide and Growth Regulators. In: Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues (1st Edition), Teixeira da Silva JA (Ed.), Global Science Books, London, UK, 3: 37-43. ISBN 4903313069;
  9. Carimi F (2005) Chapter 26. Somatic embryogenesis protocol: Citrus. In: Protocol for Somatic Embryogenesis in Woody Plants, Jain SM & Gupta PK (Eds.). Springer, Dordrecht. Vol 77 pp 321-343. ISBN 9781402029844, DOI 10.1007/1-4020-2985-3_26;
  10. Carimi F, De Pasquale F (2003) Chapter 20. Micropropagation of Citrus. In: Jain M & Ishii K (Eds.), Micropropagation of woody trees and fruits. Kluwer Academic Publishers, Boston/Dordrecht/London. Vol 75 pp 589-619. ISBN 9789401039642. DOI 10.1007/978-94-010-0125-0_20;
  11. Carimi F, De Pasquale F, Fiore S, D’Onghia AM (2001) Sanitation of citrus germplasm by somatic embryogenesis and shoot-tip grafting. In: Improvement of the citrus sector by the setting up of the common conservation strategies for the free exchange of healthy citrus genetic resources. In D’Onghia AM, Menini U & Martelli GP (Eds.). Bari: CIHEAM-IAMB, Options Méditerranéennes: Série B. Etudes et Recherches, 33: 61-65. ISBN 2853522261;
  12. Carimi F, De Pasquale F, D’Onghia AM (2001) Conservation strategies of citrus germplasm: in vitro and in vivo. In D’Onghia AM, Menini U & Martelli GP (Eds.) Improvement of the citrus sector by the setting up of the common conservation strategies for the free exchange of healthy citrus genetic resources. Bari: CIHEAM-IAMB, Options Méditerranéennes: Série B. Etudes et Recherches, 33: 67-72. ISBN 2853522261;
  13. Carimi F (2001) Somatic embryogenesis and organogenesis in citrus for sanitation and in vitro In: D’Onghia AM, Menini U & Martelli GP (Eds.) Improvement of the citrus sector by the setting up of the common conservation strategies for the free exchange of healthy citrus genetic resources. Bari: CIHEAM-IAMB, Options Méditerranéennes: Série B. Etudes et Recherches, 33: 115-128. ISBN 2853522261;
  14. D’Onghia AM, Djelouah K, Carimi F, De Pasquale F, Savino V, Catalano L, Scillipoti O (2001). Country programmes. Italy. In D’Onghia AM, Menini U & Martelli GP (Eds.) Improvement of the citrus sector by the setting up of the common conservation strategies for the free exchange of healthy citrus genetic resources. Bari: CIHEAM-IAMB, Options Méditerranéennes: Série B. Etudes et Recherches, 33: 171-179. ISBN 2853522261.

Publications, Books and Articles

The full list of my publications is at the following URL:

http://scholar.google.it/citations?user=b0V2F2QAAAAJ&hl=en

http://www.cnr.it/people/francesco.carimi

https://www.researchgate.net/profile/Francesco_Carimi

http://ibbr.cnr.it/ibbr/people/francesco-carimi

 

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(full list available at CNR People)

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