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Institute of Biosciences and Bioresources

National Research Council of Italy

Diana Zuluaga

Role: research fellow
Section: Contract Staff
Division: Bari
Tel: (39) 080-5583400-222
E-mail: diana.zuluaga@ibbr.cnr.it


Work experience

Dates 15 Sep 2013 - ongoing
Position and employer Post-doc researcher at IBBR-CNR, Via G. Amendola, 165/A 70126 Bari.
Main activities and responsibilities
miRNA isolation and analysis in response to abiotic stress in Triticum durum
Reference Dr Gabriella Sonnante, gabriella.sonnante@ibbr.cnr.it


Dates 15 Dec 2012 - 14 Mar 2013
Position and employer Post-doc researcher at “Università degli Studi di Bari “A. Moro”, Dipartimento di Scienze del suolo, della pianta e degli alimenti”, Via G. Amendola, 165/A 70126 Bari
Main activities and responsibilities Characterisation of resistance to Orobanche spp. in Pisum sativum
Reference Dr Stefano Pavan, stefano.pavan@uniba.it


Dates 22-23 Mar 2012
Position and employer Research visit at the University of Nottingham, Plant and Crop Sciences Division, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD
Main activities and responsibilities Analysis of overexpression data of Brassica oleracea genes from two lines (several overexpressors of MYB29 and wild-type), through the use of Affymetrix Brassica Exon 1.0 ST Array and of the GeneSpring GX software.
Reference Dr Martin R. Broadley, martin.broadley@nottingham.ac.uk



Dates 27 Feb 2012 - 20 Mar 2012
Position and employer Research visit at the University of Reading, Department of Food and Nutritional Sciences, PO Box 226, Whiteknights, Reading, RG6 6AP, United Kingdom
Main activities and responsibilities Evaluation of antigenotoxic activity on colon cancer cells of the extract of a B. oleracea MYB29 overexpressor, through the comet assay (single cell electrophoresis).
Reference Dr Carol Wagstaff, c.wagstaff@reading.ac.uk


Dates 17-21 May 2010
Position and employer Research visit at the John Innes Centre, Crop Genetics Department, Norwich, NR4 7UH, United Kingdom
Main activities and responsibilities Transformation of Brassica oleracea using Agrobacterium tumefaciens
Reference Dr Penelope Sparrow, penelope.sparrow@jic.ac.uk


Dates 18 May 2009 - 15 May 2012
Position and employer Postdoc position at Laboratory of Genetics, Wageningen University. The Netherlands
Main activities and responsibilities Project title: “Design of a decision matrix to assess GM crop impact on the detrital food web”, by using Brassica oleracea genotypes with a high content of toxic aliphatic glucosinolates.
In particular:
- developed, through qRT-PCR and Brassica array, an expression map of the genes involved in the biosynthetic pathway of aliphatic glucosinolates of different B. oleracea genotypes;
- identified and quantified with HPLC the glucosinolates produced by different genotypes;
- modified, through cloning in a binary vector and transformation with Agrobacterium tomefaciens, the biosynthetic pathway of aliphatic glucosinolates in a B. oleracea wild genotype, in order to increase the production of sinigrin, an active substance useful for soil biofumigation. I repeated this procedure on a genotype derived from broccoli, obtaining a high production of glucoraphanin;
- sequenced some genes involved in the biosynthetic pathway of aliphatic glucosinolates in four B. oleracea genotypes;
- supported other colleagues working in my research group (see acknowledgements in Wijnker E, van Dun K, de Snoo CB, Lelivelt CLC, Keurentjes JJB, Naharudin NS, Ravi M, Chan SWL, de Jong H, Dirks R, 2012. Reverse breeding in Arabidopsis thaliana generates homozygous parental lines from a heterozygous plant. Nature Genetics. Doi: 10.1038/ng.2203, 5 pp).
The project was financed by The Netherlands Organisation for Scientific Research (NWO)
Reference Dr. Mark G.M. Aarts: Mark.Aarts@wur.nl


Dates 18 Feb 2009 - 15 May 2009
Position and employer Internship. National Institute of Agricultural Botany, Cambridge, UK.
Main activities and responsibilities Contributing to the project “Mapping of morphological traits in barley using genome-wide scans”
Reference Dr. James Cockram: james.cockram@niab.com
Dr. Donal M. O’Sullivan: donal.osullivan@niab.com

Dates 18 Feb 2009 - 15 May 2009
Position and employer Internship. National Institute of Agricultural Botany, Cambridge, UK.
Main activities and responsibilities Contributing to the project “Mapping of morphological traits in barley using genome-wide scans”
Reference Dr. James Cockram: james.cockram@niab.com
Dr. Donal M. O’Sullivan: donal.osullivan@niab.com


Dates Jul 2002 - Dec 2005
Position and employer Part-time lecturer at the Biology Institute. University of Antioquia, Medellín, Colombia.
Main activities and responsibilities Teaching undergraduate students the following courses: Genetic Engineering (Lecture and/or Laboratory), General Microbiology (Lecture and/or Laboratory) and General Botany (Laboratory).
Reference Prof. Jaime de J. Calle: jcalle@matematicas.udea.edu.co


Dates 29 Jun 2000 - 15 Jun 2002
Position and employer Researcher at the Biology Institute. University of Antioquia. Medellín, Colombia.
Main activities and responsibilities Contributing to the projects “Cytogenetic Analysis in the Worker Population Exposed to Aromatics Compounds in GCB-Ecopetrol, Santander, Colombia”, “Value of Occupational Risk in Workers from Cerro Matoso S.A., Córdoba, Colombia”
Reference Prof. Mauricio Camargo: mcamargo@matematicas.udea.edu.co


Dates 1 Jan 1999 - 20 Dec 2001
Position and employer Teaching assistant at the Biology Institute and School of Dentistry. University of Antioquia. Medellín, Colombia.
Main activities and responsibilities Contributing to General Microbiology and General Biology courses.
Reference Prof. Jaime de J. Calle: jcalle@matematicas.udea.edu.co


Education and Training
Dates 1 Jan 2006- 16 Jan 2009 (dissertation to be held in June 2009)
Qualification and organization Ph.D. candidate. International Doctoral Program in Agrobiodiversity.
Sant’Anna School of Advanced Studies. Pisa, Italy.
Principal subjects/occupational skills covered Research project: “Anthocyanin production and gene expression in AtMYB75 transgenic tomato plants”, financed by the Ministry of Education, University and Research.
Relevant courses: genetic transformation, plant physiology, multivariate analysis, world agroecosystems, management of agrobiodiversity in sustainable farming systems.
Reference Dr. Silvia Gonzali: s.gonzali@sssup.it, Tel: +39-349-2616585
Prof. Pierdomenico Perata: p.perata@sssup.it


Dates Jan 2003 - 10 Dec 2005
Qualification and organization M.Sc. with emphasis in Biotechnology. University of Antioquia. Medellín, Colombia.
Principal subjects/occupational skills covered Working towards a research thesis entitled “Direct regeneration and transformation from leaf tissue of Stevia rebaudiana plants”.
Relevant courses: GMOs, plant physiology, experimental design, somatic embryogenesis, Taxonomy of VA mycorrhizal fungi, plant molecular biology, genetic expression control, tissue culture, bio-informatics.
Reference Prof. Zulma Monsalve: zmonsalv@matematicas.udea.edu.co
Prof. Lucia Atehortua: latehor@quimbaya.udea.edu.co
Prof. Aura I. Urrea T.: aurrea@matematicas.udea.edu.co


Dates 1 Jan 1996 - 12 Jul 2002
Title of qualification awarded Biologist (Bachelor’s degree). University of Antioquia. Medellín, Colombia.
Principal subjects/occupational skills covered Working towards a research thesis entitled: “GSTM1 and GSTT1 for Value Susceptibility to Gastrointestinal Cancer in a Paisa Population, Antioquia, Colombia”.
Relevant courses: Microbiology, chemistry, Ecology, Botany, Cell Biology, Cytogenetics, Molecular Genetics, Genetic Engineering, Immunology, Evolution, Economic Botany, Experimental Design.
Reference Prof. Jaime de J. Calle: jcalle@matematicas.udea.edu.co
Prof. Mauricio Camargo: mcamargo@matematicas.udea.edu.co


Other Training Courses
Dates 4 Jul 2005 - 15 Jul 2005
Qualification and organization Attended a Theoretical and Practical Course: “Metagenomics: Applications and Potentialities”. National University of La Plata, Argentina.
Principal subjects Molecular Biology, Microbiology
Reference Dr. Mario Aguilar: aguilar@biol.unlp.edu.ar


Dates 29 Sep 2004 - 26 Nov 2004
Title of qualification awarded Attended a “Training Course in Methods of Genetic Transformation and Tissue Culture of Plants”. Research and Advances Studies Center from National Polytechnical Institute. (CINVESTAV), Irapuato, Gto, Mexico.
Principal subjects Plant Tissue Culture, Genetic Transformation (A. tumefaciens and biolistic methods).
Reference Dr. Rosa Maria Rangel: rrangel@ira.cinvestav.mx


Dates 19 Sep 2004 - 24 Sep 2004
Title of qualification awarded Attended the following theoretical and practical Courses: “Micropropagation of Ornamental Plants: African Violet, Sansieveria, Cactus and Ferns”, “Applications of Radioinduced Mutagenesis in Phytoimprovement” and “In vitro Culture of Strawberry Meristems”.
Faculty of Agricultural Sciences, Autonomous University of Mexico State. Toluca, Mexico.
Principal subjects Plant Tissue Culture
Reference Dr. Maria del Carmen Mendoza Castillo: somefi@taurus1.chapingo.mx


Other skills and competences

Mother tongue Spanish

Other languages
English Fluent
Italian Fluent
French Basic

Social skills and competences Participation in National and International courses, congresses and seminars.
Used to interact in multi-linguistic and multicultural environments.
Ability to fulfill my study tasks, to accomplish deadlines and to produce high-quality results even under high workload.

Computer skills and competences Word processor (MS Word, Wordperfect), Spreadsheet (MS Excel), Agenda (MS Outlook), Presentations (MS Powerpoint), Graphic Tools (Corel Draw), ContigExpress (for sequence analysis).

Driving license Third category (cars). Number 0143770, Republic of Colombia.

Awards and Honors “Young Researcher”. University of Antioquia. 2000-2005. Awarded as a Young Researcher. 20-years Sciences School. University of Antioquia. Dec. 2000.
Scholarships International Scholarship in Agrobiodiversity. (To perform Ph.D. program). S. Anna School of Advanced Studies. Pisa, Italy.
Fellowship from The Netherlands Organisation for Scientific Research (NWO), May 2009.
Fellowship from CABBIO to attend the course “Metagenomics: Applications and Potentialities”. National University of La Plata, Argentina. 4-15 July. 2005.
Instructor-Student while pursuing Master’s Degree. Nov. 2002-Dec. 2004.
Scholarship during undergraduate Biology Program. University of Antioquia. Medellín, Colombia. 1999-2001.

Publications and congress presentations

Diana L. Zuluaga, Pascuale L. Curci, Donatella Danzi, Gabriella Sonnante. 2014. Diversità genetica e delle risposte di geni alla carenza di azoto in frumento duro. Atti del X Convegno Nazionale Biodiversità. Roma, Italy 3-5 September

Pavan S., Schiavulli A., Zuluaga D.L., Marcotrigiano A.R., Zonno V., Ricciardi F., Bracuto V., Abdelaziz S., Zaza C., Bardaro N., Lotti C., Bouwmeester H., Ricciardi L. 2013. Proceedings of the 57th Italian Society of Agricultural Genetics Annual Congress. 16-19 September 2013. ISBN 978-88-904570-3-6

Diana L. Zuluaga, Ruud Verkerk, Rieta Gols, Mark G.M. Aarts 2012. Caratterizzazione della via biosintetica dei glucosinolati alifatici in un genotipo selvatico di Brassica oleracea e potenziali benefici per l’agricoltura e la salute umana. Atti del IX Convegno Nazionale Biodiversità. Bari, Italy 6-7 September 2012 Vol. 1. p. 71-75. ISBN 2-85352-497-3

Zuluaga D.L., Graham N., van Ommen Kloeke A. E. E., Klinder A., Wagstaff C., Verkerk R. and Aarts MGM 2012. Overexpression of MYB29 Transcription Factor Increases the Beneficial Effects for Agriculture and Human Health of Brassica oleracea plants. Proceedings of the 6th International symposium on Brassica and 18th Crucifer Genetics Workshop. 12-16 November 2012, Catania, Italy. P. 113.
Diana L. Zuluaga, A. E. Elaine van Ommen Kloeke, Ruud Verkerk, Neil Graham, Wilfred F. M. Röling, Jacintha Ellers, Dick Roelofs and Mark G.M. Aarts. Biofumigation effects of a wild Brassica oleracea on beneficial soil invertebrates. Submitted to Plant & Soil
Diana L. Zuluaga, Annett Klinder, Carol Wagstaff, Neil Graham, Ruud Verkerk and Mark G.M. Aarts. Overexpression of MYB29 transcription factor greatly increases the beneficial effects for human health and agriculture of Brassica oleracea crops. In preparation for Plant Biotechnology Journal
Roelofs D., van Ommen Kloeke E., Ellers J., Zuluaga Agudelo D., Hanhart C. and Aarts M. 2012. Towards the assessment of GM crop impact on the soil detritivorous food web. ERGO 2012 Symposium 2012, 16-17 February, Amsterdam - abstract.
Cockram J., White J., Zuluaga D.L., Smith D., Comadran J. et al. 2010. Genome-wide association mapping to candidate polymorphism resolution in the un-sequenced barley genome. Proc. Natl. Acad. Sci. U.S.A., 107: 21611-21616.
Diana Zuluaga, Elaine Ommen Kloeke, Jacintha Ellers, Dick Roelofs and Mark Aarts 2010. Risk assessment of Brassica oleracea glucosinolate biosynthesis on the soil detritus food web. 17th Crucifer Genetics Workshop, BRASSICA 2010. 5-9th Sept. Saskatoon, Canada.
Cockram, J., White, J., Zuluaga, D., Mackay, I., Luo, Z., Kearsey, M., Comadran, J., Marshall, D., Ramsay, L., Thomas, B., Harrap, D., Werner, P., Tapsell, C., Balding, D.J., AGOUEB, Waugh, R., O’Sullivan, D. 2009. Genome-wide association mapping in barley: from simulations to fine-mapping. Plant and Animal Genomes XVIII Conference, 9-13th January, San Diego, USA (P).
Cockram, J., White, J., Zuluaga, D., Mackay, I., Luo, Z., Kearsey, M., Comadran, J., Marshall, D., Ramsay, L., Thomas, B., Harrap, D., Werner, P., AGOUEB, Waugh, R., O’Sullivan, D. 2009. Genome-wide association mapping of multiple morphological traits in barley. Plant Genomics European Meetings, 7-10 October, Lisbon, Portugal (T).
Diana Lucia Zuluaga, Silvia Gonzali, Elena Loreti, Chiara Pucciariello, Elena Degl’Innocenti, Lucia Guidi, Amedeo Alpi and Pierdomenico Perata 2008. Arabidopsis thaliana MYB75/PAP1 Transcription Factor induces Anthocyanin Production in Transgenic Tomato Plants. Functional Plant Biology. 35(7): 606-618.
D. Zuluaga 2008. Arabidopsis thaliana MYB75/PAP1 Transcription Factor induces Anthocyanin Production in Transgenic Tomato Plants. Oral presentation at the Plant Biotechnology Session. XVI Congress of the Federation of European Societies of Plant Biology (FESPB). Tampere-Finland. 17-22 Aug.
D. Zuluaga, S. Gonzali, E. Loreti, C. Pucciariello, E. Degl’Innocenti, L. Guidi, A. Alpi and P. Perata 2008. Arabidopsis thaliana MYB75/PAP1 Transcription Factor induces Anthocyanin Production in Transgenic Tomato Plants. Abstracts Memoirs FESPB. Physiologia Plantarum. 133(3).
D. Zuluaga, S. Gonzali, E. Loreti, C. Pucciariello, E. Degl’Innocenti, L. Guidi, A. Alpi and P. Perata 2008. Arabidopsis thaliana MYB75/PAP1 Transcription Factor induces Anthocyanin Production in Transgenic Tomato Plants. Oral and Poster presentation in the XLVII (SIFV) Congresso della Società Italiana di Fisiologia Vegetale. Scuola Superiore Sant’Anna. Pisa-Italy, 30 Giugno - 2 Luglio.
D. Zuluaga, S. Gonzali, E. Loreti, C. Pucciariello, E. Degl’Innocenti, L. Guidi, A. Alpi and P. Perata. 2008. Arabidopsis thaliana MYB75/PAP1 Transcription Factor induces Anthocyanin Production in Transgenic Tomato Plants. Oral presentation in the Genetica e Genomica del Pomodoro. Il contributo italiano. Università degli studi della Tuscia. Viterbo-Italy, 29-30 Maggio.
Zuluaga DL, Monsalve ZI, Urrea A. 2004. Stevia rebaudiana Transitory Regeneration and Transformation Mediated by Agrobacterium tumefaciens. Presentation at XX Congreso Nacional de Citogenética. Toluca-Mexico, 19-24 Sept.
Castaño-Molina E, Camargo M, Santacoloma M, Arango L, Villegas CR, Zuluaga D, Arcos-Burgos M. 2003. GSTT1 and Not GSTM1 is Associated with Gastric Cancer Incidence in Paisa Community (Colombia). Environmental and Molecular Mutagenesis. ISSN 0893-6692. 41(3): 167.
Camargo M, Zuluaga D, 2002. Genotipificación molecular en tres genes de susceptibilidad potencial al cancer gastrointestinal. Presentation at II Congreso Internacional y V Congreso Colombiano de Genética, 23-26 Aprile 2002, Bogotá, Colombia.

 

Selected Publications
(full list available at CNR People)

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