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Institute of Biosciences and Bioresources

National Research Council of Italy

Anna Valenti

Role: Researcher
Section: Researchers and Technologists
Division: Naples
Tel: (39) 081-6132496
E-mail: anna.valenti@ibbr.cnr.it


POSITION

Permanent position as Researcher at Institute of Biosciences and BioResources- National Research Council of Italy 


WORK EXPERIENCE

dates (12/2012 - to now) Permanent position as researcher

Institute of Biosciences and BioResources- National Research Council of Italy 


▪ Structure and function of proteins and enzymes involved in DNA metabolism in mesophilic and thermophilic microorganisms. Mechanisms of genome stability and repair in model organisms. Nanopores application to study DNA-protein interaction and DNA structure.

Sector Molecular Biology and Biochemistry

dates (03/2012 - 12/2012) Temporary position as researcher

Institute of Protein Biochemistry- National Research Council of Italy 


▪ Structure and function of proteins and enzymes involved in DNA metabolism in mesophilic and thermophilic microorganisms

Sector Molecular Biology and Biochemistry

dates (10/2008 - 01/2012) Post-doctoral fellow

Institute of Protein Biochemistry- National Research Council of Italy 


▪ Structure and function of proteins and enzymes involved in DNA metabolism (topoisomerases, helicases, DNA binding proteins, translesion DNA polymerases)

Sector Molecular Biology and Biochemistry

dates (10/2008 - 01/2012) Research fellow and PhD student

Institute of Protein Biochemistry- National Research Council of Italy 


▪ Mechanisms of genome stability and repair in microorganisms

Business or sector Molecular Biology and Biochemistry

EDUCATION AND TRAINING

dates (from 11/2004 - to 11/2007) PhD degree in Applied Biology

Date of graduation 07/02/2008

Institution: University of Naples “Federico II”

Title of Thesis: " Risposta UV in Sulfolobus solfataricus: regolazione della girasi inversa da danno al DNA"

dates (from 11/1996 - to 03/2004) Degree in Biology

Date of graduation 29/03/2004

Institution University of Naples “Federico II”

Title of Thesis: "La girasi inversa dell’Archeon termofilo Sulfolobus solfataricus"

dates (from 09/1991 - to 06/1996) High school diploma

Date of graduation 06/1996

Liceo Scientifico- A. Romita- Campobasso

Organisational / managerial skills/leadership

currently Principal investigator of Research Unit of reasearch project FIRB- Ministero Italiano dell’Università e Ricerca

Technical skills and competence

-Purification, biochemical analysis and engineering of proteins and enzymes involved in DNA metabolism (topoisomerases, helicases, DNA binding proteins, DNA polymerases);


-Immobilization of protein on nanopore material

-Production of DNA substrates with specific structure and topology


-Analysis of DNA topology

-In vivo and in vitro protein-protein interaction techniques (co-immunoprecipitation, pull-down, south-western blot, FRET, Surface plasmon resonance)

- In vivo and in vitro DNA-protein interaction techniques (EMSA, footprinting, crosslinking, chromatin immunoprecipitation)
- qualitative and quantitative gene expression analysis (western blot, northern blot, real-time RT-PCR)


- manipulation of bacteria, yeast and nematodes

PUBLICATIONS

Visone V., Vettone A., Serpe M., VALENTI A., Perugino G., Rossi M. and Maria Ciaramella. (2014) Chromatin structure and dynamics in hot environment: architectural proteins and DNA topoisomerases of thermophilic archaea. Review. INTERNATION JOURNAL OF MOLECULAR SCIENCES in press

Vettone A., Perugino G, Rossi M, VALENTI A, Ciaramella M. Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase. (2014) Review Extremophiles. Epub ahead of print

Jamroze A, Perugino G, VALENTI A, Rashid N, Rossi M, Akhtar M, Ciaramella M. (2013) The reverse gyrase from Pyrobaculum calidifontis, a novel extremely thermophilic DNA topoisomerase endowed with DNA unwinding and annealing activities. THE JOURNAL OF BIOLOGICAL CHEMISTRY

VALENTI A., De Felice M, Perugino G, Bizard A, Nadal M, Rossi M, Ciaramella M. (2012). Synergic and opposing activities of thermophilic RecQ-like helicase and Topoisomerase 3 in Holliday junction processing and replication fork stabilization. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

Perugino G, Vettone A, Illiano G, VALENTI A., Ferrara MC, Rossi M, Ciaramella M. (2012). Activity and Regulation of Archaeal DNA Alkyltransferase: conserved protein involved in repair of DNA alkylation damage. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

VALENTI A., Perugino G, Rossi M, Ciaramella M. (2011). Positive supercoiling in thermophiles and mesophiles: of the good and evil. BIOCHEMICAL SOCIETY TRANSACTIONS, ISSN: 0300-5127

VALENTI A., Perugino G, Varriale A, D’Auria S, Rossi M, Ciaramella M. (2010). The archaeal topoisomerase reverse gyrase is a helix-destabilizing protein that unwinds four-way DNA junctions. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

VALENTI A., Perugino G, Nohmi T, Rossi M, Ciaramella M. (2009). Inhibition of translesion DNA polymerase by archaeal reverse gyrase. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Perugino G, VALENTI A., D’amaro A, Rossi M, Ciaramella M. (2009). Reverse gyrase and genome stability in hyperthermophilic organisms. BIOCHEMICAL SOCIETY TRANSACTIONS, ISSN: 0300-5127

D’Amaro A, VALENTI A., Napoli A, Rossi M, Ciaramella M. (2008). The prefoldin of the crenarchaeon Sulfolobus solfataricus. PROTEIN AND PEPTIDE LETTERS, ISSN: 0929-8665

VALENTI A., Perugino G, D’Amaro A, Cacace A, Napoli A, Rossi M, Ciaramella M. (2008). Dissection of reverse gyrase activities: insight into the evolution of a thermostable molecular machine. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Romano V, Napoli A, Salerno V, VALENTI A., Rossi M, Ciaramella M. (2007). Lack of strand-specific repair of UV-induced DNA lesions in three genes of the archaeon Sulfolobus solfataricus. JOURNAL OF MOLECULAR BIOLOGY, ISSN: 0022-2836

VALENTI A., Napoli A, di Simone C, Ferrara MC, Romano V, Rossi M and Ciaramella M (2006). Reverse gyrase and DNA damage response in thermophilic Archaea . ITALIAN JOURNAL OF BIOCHEMISTRY, ISSN: 0021-2938

D’Amaro A, VALENTI A., Napoli A, Rossi M and Ciaramella M (2006). Characterization of a thermophilic molecular chaperone: The prefoldin of the crenarchaeon Sulfolobus solfataricus. ITALIAN JOURNAL OF BIOCHEMISTRY, ISSN: 0021-2938

VALENTI A., Napoli A, Ferrara MC, Nadal M, Rossi M, Ciaramella M. (2006). Selective degradation of reverse gyrase and DNA fragmentation induced by alkylating agent in the archaeon Sulfolobus solfataricus. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Napoli A, VALENTI A., Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M. (2005). Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus. NUCLEIC ACIDS RESEARCH, ISSN: 0305-1048

Napoli A, VALENTI A., Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M. (2004). Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus. THE JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN: 0021-9258

PROJECTS

-FIRB-Futurio in Ricerca (2013-2016): Materiali Nanoporosi: lavagnE autoasseMblAnti per lo sTudio della struttura e delle Interazioni del DNA (NEMATIC). PI of Research Unit: Anna Valenti

-TELETHON (2012-2014): New pharmacological targets in Fanconi Anemia. PI of Research Unit: Maria Ciaramella

-AIRC (2011-2013): Cisplatin sensitivity/tolerance/resistance genetic pathways in hereditary cancer predisposition. PI of Research Unit: Maria Ciaramella

 

Selected Publications
(full list available at CNR People)

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