National Research Council of Italy

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IBBR publication #1897

Multiplex IgE diagnostic test: the most comprehensive view on IgE sensitization to vegetables, fruits and seeds due to lipid transfer proteins

Alessandri C, Giangrieco I, Tuppo L, Ferrara R, Zennaro D, Bernardi ML, Rafaiani C, Ciancamerla M, Tamburrini M, Ciardiello MA, Mari A

Allergy 72: 664-664. (2017)

Introduction:The plant LTP family consists of ubiquitous proteinstypically involved in defense mechanisms against the attack of bacte-ria, fungi and viruses. They constitute an important cause of allergicsensitization in the Mediterranean area. A new nanobead-based IVDtest for specific IgE detection using 122 molecular allergens and 122allergenic extracts, all coupled to chemically activated nano-particles.Eight LTP purified from kiwi, hazelnut, pomegranate, peach, walnut,corn, tomato and wheat are immobilized together with 25 extractsfrom plant-derived foods bearing, along with other allergens, theirLTP.Objectives:To describe the LTP sensitization in a population of1751 patients by using molecules and extracts.Results:Out of the 1751 routinely tested patients 208 (11.88%)turned out to be sensitized to at least one LTP. The sensitizationprevalence of each specific LTP within the 208 patients wasdetected as follows: Pru p 3 94%, Jug r 3 49%, Cor a 8 30%, Zea m14 30%, Pun g 1 23%, Act d 10 21%, Tri a 7k-LTP 3%, Sola l 6 2%.Pru p 3 reaches 100% prevalence in pediatric patients between 0 to10 years old population. The 6% Pru p 3 negative subjects turnedout to be mono-sensitized to one of the other tested LTP. The avail-ability of extracts from many other vegetables, fruits and seedsallowed the definition of the sensitization to a broader number ofrelevant LTP. An IgE reactivity was detected to allergenic sourceswhose content in LTP is still unknown. A different IgE reactivity hasbeen recorded for the 9 k-LTP and the 7 k-LTP. Patients recognizingthe latter group have a broader recognition pattern and higher IgElevels.Conclusions:The specific advantage of a MULTIPLEX isle testrelies on the chance of testing patients to a broader panel of LTP aswell as to a large number of extracts, complementing the results onthe single allergenic molecules.The availability of the MULTIPLEX test of allergens belonging to theBet v 1-like proteins, Profilins, CCD markers and Anti-Microbial Pep-tide groups allows to greatly improve the patient sensitization profil-ing. The use of extracts to consumed food or newly introduced onesincreases our knowledge on allergens.

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