National Research Council of Italy

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IBBR publication #1891

Multiplex IgE diagnostic test: IgE sensitization to furry animal dissected by allergenic molecules and extracts

Alessandri C, Zennaro D, Rafaiani C, Ferrara R, Bernardi ML, Ciancamerla M, Giangrieco I, Tuppo L, Tamburrini M, Ciardiello MA, Mari A

Allergy 72: 662-662. (2017)

Introduction:Exposure to animal allergens is a risk factor for thesensitization and symptoms. The risk of unpredictable symptoms ishigher when the patient does not know her/his sensitization and forinstance go for buying a pet or begins a job with high exposure toanimal shedding. The test is a new multiplex test for specific IgEdetection using 122 molecular allergens and 122 allergenic extracts,coupled to chemically activated nano-particles.Objectives:To investigate profiles of animal epithelia sensitizationby means of allergenic molecules and extracts immobilized on themultiplex test.Results:The tested allergenic preparations, all spotted on the mul-tiplex array, were eight epithelia extracts (dog, guinea pig, hamster,horse, cat, mouse, rabbit, rat), seven genuine allergenic proteins (Canf 1, Can f 2, Can f 5, Equ c 1, Fel d 1, Mus m 1, Rat n 1). Out of the1751 routinely tested patients 568 (32.44%) turned out to be sensi-tized to at least one of the 15 tested allergenic preparation. Themost recognized extract was cat epithelium with 57% sensitizationprevalence. The least recognized extract is horse epithelium with1.4% sensitization prevalence. The most recognized protein is Fel d1 with 63% sensitization prevalence. The least recognized proteinsare Mus m 1 and Rat n 1 with 0.4% sensitization prevalence each. Itis worth noting that around 5% of the 568 patients are also sensi-tized to serum albumins (Bos d 6, Can f 3, Equ c 3, Fel d 2, Mus m4, Ory c 6, Rat n 4, Sus s 1) with several different patterns of IgErecognition.Conclusions:The present study results confirm differences in sen-sitization towards different animal epithelia. Allergy testing using sin-gle species markers, serum albumin and epithelium extracts whenmolecules are not available yet, might help in decision making in theneed for choosing future non risky exposures to furry animals. Thesensitization to serum albumin creates a cluster of IgE co-reactivitythat must be clinically interpreted in the light of the inhalation of theunprocessed allergen. The use of homologous lipocalin, showingdivergent IgE binding in routinely tested patients, might help inunderstanding the IgE epitope distribution in this group of allergens.

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