National Research Council of Italy

Institute of Biosciences and BioResources

DISBA logo CNR logo
IBBR publication #1877

Introducing a multiplex IgE diagnostic test, a new nanobead-based tool for allergy diagnosis: reporting on IgE reactivity of single allergen preparations and reproducibility performances

Mari A, Mitterer G, Rafaiani C, Ciancamerla M, Bernardi ML, Alessandri C, Tuppo L, Giangrieco I, Ferrara R, Zennaro D, Tamburrini M, Ciardiello MA, Harwanegg C

Allergy 72: 117-117. (2017)

Introduction:Allergy diagnosis is currently based on the use ofpurified allergenic proteins and protein extract. Diagnosis can be per-formed in vivo using a limited number of extracts, whereas all aller-genic preparations can be tested in vitro by measuring specific IgE.In addition to third generation singleplex lab tests, multiplex testingtools are available since 10 years, allowing to have many IgE resultsfrom a single sample by testing allergens as micro spots on a rigidsurface. Recently a new generation of lab diagnostic device has beenreleased, the FABER test, adopting modern nanotechnologies,namely nanobeads, for allergen immobilization.Objectives:To report the general set up of a multiplex test andthe evaluation of some of its performances.Results:The test is based on 244 molecules (122) and extracts (122),coupled to nano-particles. Particles are arrayed to a solid phase matrix,to form a one-step comprehensive array-based testing solution, using120ll of serum per test. Each allergen particle population can be indi-vidually optimized to achieve the maximum testing performance. Theproof of the IgE binding for the 244 allergen preparations has beenobtained by using routine serum samples from the sera bank. The eval-uation of the IgE binding was obtained by using a standard polyclonalcommercial preparation obtained by pooling human sera. The test wasused on 22 consecutive batches. 243 allergen preparations, even therarest one in term of prevalence in the general population, gave posi-tive IgE result using single serum samples. Negative results arerecorded for HSA which is the negative control test. 174 out of 244allergens gave positive IgE results. Average CV values were between15 and 25%. Allergen specificities having values in the lower IgE rangehad a lower reproducibility rather than those in the higher range, any-how never dropping to negative IgE detection. The multiplex testreproducibility performance was not related to the kind of allergenpreparations, namely purified molecule or verified extracts.Conclusions:This test is a new lab test for multiplex specific IgEdetection using allergenic molecules and extracts at the same timeshowing good performances. All steps in assembling the test are ver-ified and the present study reports that all allergens bind IgE. Evalua-tion of missing allergen specificities is performed with in-housemade serum pools and performances could be further improved.ABSTRACTS|1

Actions
Select by Year
Select by Type
Select by Author
*
*
*
*
Istituto di Bioscienze e Biorisorse (IBBR/CNR)
Via G. Amendola 165/A, I-70126 Bari (Italy)
Copyright © 2012-2024. All Rights Reserved.