National Research Council of Italy

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IBBR publication #1858

Faber IgE test: a standard multiplex diagnostic tool to explore allergic sensitization across different European regions: Romania, Croatia, Italy

Miskovic S, Nicolae CA, Grgic P, Zennaro D, Ferrara R, Bernardi ML, Rafaiani C, Ciancamerla M, Tamburrini M, Giangrieco I, Tuppo L, Ciardiello MA, Mari A, Alessandri C

Allergy 72: 354-355. (2017)

Introduction:IgE-mediated Allergic diseases are progressivelyaffecting more people globally. The allergenic sources vary from onegeographic area to another. To compare the prevalence of IgE sensi-tization between Countries the diagnostic tests should be pre-defined in terms of allergenic preparations and be as comprehensiveas possible. The use of a broad panel of allergens facilitates thestudy design. The FABER test for IgE detection bearing 244 aller-gens, both molecules (122) and extracts (122), is the most advancedtool for this purpose including inhalant and food allergens.Objectives:A pilot study has been performed comparing IgE sensi-tization in three European Countries (Croatia, HR; Romania, RO;Italy, IT). FABER 244 version has been used to detect specific IgE.Serum samples were all tested in a single lab for the routine diagnos-tic workup. The three groups of 68 (HR), 60 (RO) and 69 (IT)patients were matched for both age (0.6-75 yo, average: 28 yo) andgender distribution (F/M=1.11:1).Results:The sensitization for all dust mite allergens appears to behomogeneous in the three groups with the highest values in RO forDer p 2 (45%) and the lowest in HR for Der p 7 (1.5%). Der p 23,showed a significant difference in prevalence between IT (26.1%)and RO (8.3%). Grass, Fagales, Parietaria, Olive and Cypress pollenmarker allergens were as follows: Bet v 1 (HR 7%, IT 10%, RO 5%),Par j 2 (HR 6%, IT 16%, RO 2%), Ole e 1 (HR 18%, IT 22%, RO 5%),Cup a 1 (HR 29%, IT 46%, RO 23%), Phl p 1 (HR 13%, IT 36%, RO13%), Phl p 5 (HR 7%, IT 17%, RO 2%), and Amb a 1 (HR 9%, IT 0%,RO 15%). The allergenic molecules bearing CCD appear to be morerecognized by RO patients, whereas LTP sensitization was recordedhigher in RO (15%) compared to IT (13%) and HR (9%). Other aller-gen sensitizations were: Tropomyosin (HR 6%, IT 3%, RO 3%); Pro-filin (HR 4%, IT 7%, RO 5%); Parvalbumin (HR 6%, IT 3%, RO 2%).Conclusions:IgE detection by means of the FABER multiplex testallows to precisely detect sensitization prevalence to inhalant andfood allergens using data from the routine diagnosis. FABER testingpreliminary discloses differences and similarities among differentCountries having different climates and eating habits. Due to the354|ABSTRACTS small number of observations, unwanted biases could be introducedin the patient enrolled in this preliminary study. To draw any conclu-sion the study using the same FABER multiplex tool on largercohorts of patients will be performed.

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