Comparison of the sulfonamide inhibition profiles of the alpha-, beta- and gamma-carbonic anhydrases from the pathogenic bacterium Vibrio cholerae
Del Prete S, Vullo D, De Luca V, Carginale V, Osman SM, Alothman Z, Supuran CT, Capasso C
Bioorganic and medicinal chemistry letters Pergamon, Paese di pubblicazione: Regno Unito Lingua: inglese ISSN: 0960-894X 26 (8): 1941-1946. (2016) doi: 10.1016/j.bmcl.2016.03.014
Carbonic anhydrases (CA, EC 4.2.1.1) are ubiquitous metalloenzymes, which catalyze the conversion of carbon dioxide (CO2) to bicarbonate (HCO3) and protons (H+). In prokaryotes, the existence of genes encoding for alpha-, beta- and gamma-classes suggests that these enzymes play an important role in the prokaryotic physiology. It has been demonstrated, in fact, that their inhibition in vivo leads to growth impairment or growth defects of the microorganism. Ultimately, we started to investigate the biochemical properties and the inhibitory profiles of the alpha- and beta-CAs identified in the genome of Vibrio cholerae, which is the causative agent of cholera. The genome of this pathogen encodes for CAs belonging to alpha, beta and gamma classes. Here, we report a sulfonamide inhibition study of the gamma-CA (named VchCA gamma) comparing it with data obtained for the alpha- and beta-CA enzymes. VchCA gamma activity (k(cat) = 7.39 x 10(5) s(-1)) was significantly higher than the other gamma-CAs. The inhibition study with a panel of sulfonamides and one sulfamate led to the detection of a large number of nanomolar VchCA gamma inhibitors, including simple aromatic/heterocyclic sulfonamides (compounds 2-9, 11, 13-15, 24) as well as EZA, DZA, BRZ, BZA, TPM, ZNS, SLP, IND (K(I)s in the range of 66.2-95.3 nM). As it was proven that bicarbonate is a virulence factor of this bacterium and since ethoxzolamide was shown to inhibit this virulence in vivo, we propose that VchCA, VchCA beta and VchCA gamma may be a target for antibiotic development, exploiting a mechanism of action rarely considered up until now, i.e., interference with bicarbonate supply as a virulence factor. (C) 2016 Elsevier Ltd. All rights reserved.
