National Research Council of Italy

Institute of Biosciences and BioResources

DISBA logo CNR logo

Eleonora Palagano

Role: Researcher
Section: Researchers and Technologists
Division: Florence
Tel: (39) 0555225727
E-mail: eleonora.palagano@ibbr.cnr.it

Country: Italy
Email: eleonora.palagano@cnr.it
eleonora.palagano@ibbr.cnr.it

Education

2019. Qualified as Professional Biologist, University of Florence
2015-2018. Phd in experimental medicine and medical biotechnology, University of Milan
2010-2012. Master degree in Pharmacology and Medical Biotechnology, University of Florence (Italy)
110/110 cum laude
experimental thesis in the laboratory of Biochemistry, Trinity College, Dublin (Ireland)
2007-2010. Degree in Medical Biotechnology, University of Florence (Italy)
110/110 cum laude
experimental thesis in the laboratory of Biochemistry, University of Florence, Florence (Italy)

Work Experience

2019-present
Tenured Scientist, Research III level at CNR Institute of Biosciences and Bioresources (IBBR) UOS Florence, Italy

2018-2019
Post-Doc research fellow at laboratory Human Genome CNR-UOS, Istituto Clinico Humanitas, Rozzano (MI), Italy
“Systems biology for the functional validation of the genetic determinants of skeletal diseases”

1) Identification of a new mutation in NBAS gene in two patients affected by a rare disease, acrofrontofacionasal dysostosis type 1
2) Generation and phenotypical characterization of a novel transgenic mouse model carrying NBAS intronic mutation
3) Bone characterization of a novel transgenic mouse model

2015-2018
Phd student in experimental medicine and medical biotechnology, Istituto Clinico Humanitas, Rozzano (MI), Italy

“New insights in bone biology from exome sequencing of rare skeletal diseases”
1) Identification of synonymous mutations in two well-known genes associated with osteopetrosis disease.
2) Exome sequencing analysis to describe a novel homozygous mutation in the FERMT3 gene in an osteopetrotic patient corrected by hematopoietic stem cell transplantation
3) Analysis of the osteogenic differentiation impairment in Rankl deficient cells

2014
Laboratory Human Genome CNR-UOS, Istituto Clinico Humanitas, Rozzano (MI), Italy

“Systems biology for the functional validation of the genetic determinants of skeletal diseases”
1) Exome sequencing analysis of osteopetrotic patients and in vitro validation
2) Identification of a deep intronic mutation in two siblings affected by osteopetrosis

2013
Laboratory of proteomics, IFOM the FIRC Institute of Molecular Oncology Foundation, Milan, Italy
“Sp140 PHD Finger as a potential target for chronic lymphocytic leukemia”

1) Study of the biological role of Sp140 protein by mass spectrometry analysis

Technical skills and competences

Scientific writing (reports, grant proposal) and literature database screening
Biochemistry: immunoblotting, immunoprecipitation, pull down, Mass Spectrometry (MS) analysis
Molecular biology: site direct mutagenesis, Polymerase Chain Reaction (PCR), molecular cloning, DNA and RNA extractions from cells, Peripheral Blood Mononuclear Cells (PBMC) purification, electrophoresis of nucleic acids
Cell biology: cell culture, immunofluorescence, immunohistochemistry, transient gene expression, cell viability studies, osteoclast differentiation
Softwars and programs: Microsoft Office programs Excel, Word and PowerPoint, Human Splicing Finder, Primer3, ApE-a plasmide editor, Photoshop, GraphPad Prism7, ImageJ
Languages: Italian mother tongue, good spoken and written English

Awards and Grants

May 2017. 44th ECTS CONGRESS NEW INVESTIGATOR AWARD. Salzburg, Austria.
May 2018. Travel Award 7th Osteoimmunology Conference. Crete, Greece.
May 2019. ECTS Travel Award. Budapest, Hungary.

Publications

  • Capo V. et al., Expanded circulating hematopoietic stem/progenitor cells as novel cell source for the treatment of TCIRG1 osteopetrosis, Haematologica. 2020 Jan
  • Palagano E. et al., Generation of an immunodeficient mouse model of tcirg1-deficient autosomal recessive osteopetrosis, Bone Rep. 2020 Jan
  • Menale C. et al., Absence of Dipeptidyl Peptidase 3 increases oxidative stress and causes bone loss, J Bone Miner Res. 2019 Jul
  • Penna S. et al., One Disease, Many Genes: Implications for the Treatment of Osteopetroses, Front Endocrinol (Lausanne). 2019 Feb
  • Sobacchi C. et al., 3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells, Int J Mol Sci. 2018 Oct
  • Menale C. et al., MSC-seeded biomimetic scaffolds as factory of soluble RANKL in Rankl-deficient osteopetrosis, Stem Cells Transl Med. 2018 Sep
  • Palagano E. Zuccarini G. et al., Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1, Bone. 2018 Jun
  • Palagano E. et al., Genetics of osteopetrosis, Curr Osteoporos Rep. 2018 Feb;16(1):13-25
  • Faggioli F. et al., B lymphocytes limit senescence-driven fibrosis resolution and favor hepatocarcinogenesis in mouse liver injury, Hepatology. 2017 Nov 3
  • Sobacchi C. et al., Soluble factors on stage to direct mesenchymal stem cells fate, Front Bioeng Biotechnol. 2017 May 17;5:32.
  • Schena F. Menale C. et al., Murine Rankl-/- Mesenchymal Stromal Cells display an osteogenic differentiation defect improved by a RANKL-expressing lentiviral vector, Stem Cells. 2017 May;35(5):1365-1377
  • Palagano E. et al., Hematopoietic Stem Cell Transplantation corrects Osteopetrosis in a child carrying a novel homozygous mutation in the FERMT3 gene, Bone. 2017 Jan 14;97:126-129
  • E. Palagano et al., Synonymous mutations add a layer of complexity in the diagnosis of human Osteopetrosis, J Bone Miner Res. 2017 Jan;32(1):99-105
  • Palagano E. et al., Buried in the middle, but guilty: intronic mutations in the TCIRG1 gene cause human Autosomal Recessive Osteopetrosis, J Bone Miner Res. 2015 Oct;30(10):1814-21
  • O’Callaghan K. et al., Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1.5-benzoxazepines, Mol Med Rep. 2015 Sep;12(3):3748-54
  • Zucchelli C. et al., Structure of human Sp140 PHD finger: an atypical fold interacting with Pin, FEBS J. 2014 Jan;281(1):216-31

Oral presentations

  • Mutations in the Neuroblastoma Amplified Sequence (NBAS) gene in Acrofrontofacionasal Dysostosis type 1, Forum in Bone and Mineral Research 19th Meeting (February, 28 - March 1, 2019) Milan, Italy
  • Invited Speaker: Rare... rarissime anzi possibili: le osteopatie rare, una sfida multidisciplinare, XVIII Congresso Nazionale SIOMMMS (October 25-27, 2018) Naples, Italy
  • NBAS gene mutation in two patients affected by Acrofrontofacionasal Dysostosis type 1, Sybil Satellite Meeting (May 13-16, 2017) Salzburg, Austria
  • Peculiar osteopetrotic patients: new insights on bone biology from exome sequencing, 44th Annual European Calcified Tissue Society Congress (May 13-16, 2017) Salzburg, Austria
  • NBAS gene mutation in two patients affected by Acrofrontofacionasal Dysostosis type 1, Forum in Bone and Mineral Research 17th (February 24-25, 2017) Rome, Italy
  • Atypical mutations in the diagnosis of human osteopetrosis: an additional layer of complexity, Workshop Biometra (September 26, 2016) Milan, Italy
  • Synonymous but not silent mutations in the diagnosis of human Osteopetrosis: an additional layer of complexity, Forum in Bone and Mineral Research 16th Meeting (June 15-16, 2016) Torino, Italy
  • NBAS is the gene mutated in two patients affected by Acrofrontofacionasal Dysostosis type , 2nd Skeleton meeting (May 12-13, 2016) Rome, Italy
  • New insights on bone biology from exome sequencing of osteopetrotic patients, ECTS PhD Training (September 13-16, 2015) Siena, Italy
  • Intronic mutations in the TCIRG1 gene cause human Autosomal Recessive Osteopetrosis, Forum in bone & mineral research (March 6-7, 2015) Torino, Italy
  • Exome sequencing of osteoimmunological diseases: from diagnosis to cure of osteopetrosis and other genetic skeletal diseases, 1st Skeleton meeting (June 10-11, 2015) Milan, Italy
  • Molecular diagnosis of osteopetrotic patients with atypical presentations using traditional approaches and exome sequencing, Forum in bone & mineral research (May 23-24, 2014) Siena, Italy

Conference and school

  • Training Course on RNA-Seq Data Analysis, July 18-20, 2017 University of Milan, Italy
  • NBAS gene mutation in two patients affected by Acrofrontofacionasal Dysostosis type 1, Humanitas Research Day, May 3, 2017 Milan, Italy (Poster)
  • Course on Exome analysis with GALAXY, September 19-20, 2016 Milan, Italy
  • PhD training ECTS, July 2-5, 2016 Oxford, United Kingdom (Poster)
  • 43rd Annual European Calcified Tissue Society Congress, May 14-17, 2016 Rome, Italy (Poster)
  • Humanitas Research Day, May 10, 2016 Milan, Italy (Poster)
  • Data analysis Next Generation Sequencing in Galaxy, November 18-20, 2014 Pula (CA), Italy
  • 3rd course in Next Generation Sequencing, May 6-10, 2014 Bertinoro, Italy (Poster)
  • Late Summer Practical Proteomics Seminar, September 2-3, 2013 IMP/IMBA, Vienna, Austria
 

Selected Publications

(full list available at CNR People)

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