National Research Council of Italy

Institute of Biosciences and Bioresources

Donatella Danzi

Role: research fellow
Section: Contract Staff
Division: Bari
Tel: (39) 0805583400-222
E-mail: donatella.danzi@ibbr.cnr.it


Personal Information

First name(s)/ Surname(s): Donatella Danzi

Address Street : Piazza Mattarella, 3

postcode: 75012, Bernalda (MT), Italy

Mobile number: 3207948605

E-mail address: donatella.danzi@ibbr.cnr.it donatella.danzi@pec.it

Nationality: Italian

Date of birth: 26/03/1984

Research interests: Plant Genomics, Plant Phenomics, Biodiversity, Biochemistry

Professional Experience

Date: ( 01/07/2017 - present)

Short-Term Scientific Missions (STSM) COST Action FA 1306

Research Project: “Mining of new below-ground traits in a durum wheat germplasm collection. Analysis of the response to water stress in a collection of durum wheat genotypes using phenotyping platform”.

Institute: Institute of Bio- and Geosciences Plant Sciences (IBG-2) Forschungszentrum Jülich Wilhelm- Johnen-Straße 52428 Jülich (Germany).

Date: ( 25/02/2017 - 30/06/2017)

Scholarship of the Department of Biology, Agriculture and Food Science - National Research

Council of Italy (DISBA - CNR) (Award DISBA 2016).

Research Project: “Mining of new below-ground traits in a durum wheat germplasm collection.”

Institute: Institute of Bio- and Geosciences Plant Sciences (IBG-2) Forschungszentrum Jülich Wilhelm- Johnen-Straße 52428 Jülich (Germany).

Date: (01/09/2015 - 24/02/2017)

Fellowship n. IBBR-BS-003-2015-BA

Research Project: R.G.V. - FAO IV - “International Treaty on Plant Genetic Resources for Food and Agriculture " and CISIA “ integrated knowledge for sustainability and innovation of Made in Italy food ". Biochemistry and genetic characterization of biomass fiber components (lignin, cellulose, hemicellulose, etc.) in a collection of durum wheat genotypes. Analysis of the response to water stress in a collection of durum wheat genotypes using phenotyping Lemnatech platform.

Institute: Joint Research Unit (ALSIA-IBBR) - Institute of Biosciences and Bioresources, National Research Council (CNR) - Metapontum Agrobios.

Date: ( 01/02/2014 - 31/05/2015)

Postdoctoral Fellow n. IBBR-001-2014-BA

Research Project: PON 01145 ISCOCEM - “Technological Development and Innovation for the sustainability and competitivity of South Italy Cereal Genetics”. Isolation and characterization of genes involved in nitrogen metabolism in durum wheat .

Institute: Biosciences and Bioresources, National Research Council (CNR) - Bari - Street G. Amendola 165/A 70126 Bari .

Date: (15/09/2010 - 15/12/2010)

Continued Coordinated Collaboration

Institute: Biosciences and Bioresources, National Research Council (CNR) - Bari - Street G. Amendola 165/A 70126 Bari .

Date: ( 01/03/2010 -31/07/2010)

Training

Institute: Council for Research and Experimentation in Agriculture CRA-SCA - Bari - Street Celso Ulpiani 5, 70125 Bari

Education

Date: ( 01/01/2011 - 31/12/2013)

Ph.D. in Plant Genetics and Biotechnology, School of Plant Science and Enviromental Technologies , University of Bari “ A. Moro” .

Research Project: "Screening BAC library of artichoke and characterization of some genes isolated".

Institute: Biosciences and Bioresources, National Research Council (CNR) - Bari - Street G. Amendola 165/A 70126 Bari .

Date: 2011

Qualification to the profession of Biologist, University of Bari “ A. Moro” - Bari (Italy)

Date: (2007-2009)

M.S. in Genomic Biotecnology , final mark 110 /110 e lode

University of Rome “La Sapienza” - Rome (Italy)

Thesis title: “Study of changes of pectic component to improve the enzymatic saccharification of plant biomass”.

Date: (2003 - 2007)

B.S. in Biomedic Biotechnology, final mark 105/110

University of Basilicata - Potenza (Italy)

Thesis title: “Helicobacter and autoimmune diseases: correlation with Hashimoto’s thyroiditis”

Languages

Italian: mother tongue

English: fluent

Laboratory skills

DNA: extraction, digestion, quality and concentration analysis, purification, sequencing. Gene isolation techniques and structural characterization. Labelling (fluorescent probe) and detection. Recombinant DNA techniques.

Bacterial Artificial Chromosomes (BAC): BAC library screening, plasmid extraction, BAC clones analysis .

RNA e microRNA: extraction, electrophoresis, quality and concentration analysis, retrotrascription.

Polymerase Chain Reaction (PCR): standard, real time. High Resolution Melt (HRM): microsatellite and SNP analysis.

Electrophoresis techniques: band cutting and purification agarose gel, SDS-PAGE for protein analysis, EMSA (Electrophoretic mobility shift assay) for interaction protein/DNA.

Protein: purification, quantification, characterization.

Cell wall analysis: extraction, determination of total sugars in the wall, extraction of pectin component, determining the degree of methylation of the pectin, immunochemical assays to determine different epitopes pectic using antibodies monoclonal, cup plate to assess enzymatic activity, pretreatment and saccharification of plant biomass, extraction and quantification of lignin content in plant biomass.

Green house activity: hydroponic cultivation of wheat, knowledge of phenological phases and morphological analysis related, analysis stomatic conductance by means leaf porometer, determination of green index by means SPAD o DUALEX, sampling methods, analysis for determination leaf relative water content (RWC).

Phenomics: High throughput phenotyping approach for precise characterization and selection of the germplasm collection and for the identification of more adaptable varieties.

Root phenotyping : study of root architecture by blotting paper and rhizotron system.

Statistical analysis

Descriptive statistics, Software SigmaPlot for PCA analysis, analysis of variance

Computer skills

ECDL (European Computer Driving License) Bioinformatics: sequence manipulation and analysis (Chromas), international database (NCBI, EMBl), multi alignment (ClustalW, Blast), structural characterization of genes and promoters ( Softberry, Place promoter, Plantpan promoter), phylogenetic analysis (MEGA).

Advanced Courses Attended & Roles Covered At International Events

  • 54th S.I.G.A. Annual Congress. Matera, 27-30 september 2010  Symposium: “ Empowering New Discoveries trough Next-Generation Sequencing” Bari, 01 june 2010
  • Advanced course: "Using diversity to understand fundamental processes of speciation, genome organization " . Viterbo , 20-22 june 2011
  • 57th S.I.G.A. Annual Congress. Foggia, 16-19 september 2013  Advanced course: “’From genetic diversity to molecular plant breeding through "OMIC" studies” 3rd Annual Meeting PGB Network. Bari, 9-13 june 2014  X National Conference on Biodiversity. Roma, 3-5 september 2014
  • MiSeq Illumina training. Bari, 22-23 january

Publications & Posters presented at International Conference

  • COMPLETE CHLOROPLAST GENOME OF THE MULTIFUNCTIONAL CROP GLOBE ARTICHOKE AND COMPARISON WITH OTHER ASTERACEAE CURCI P.L, DE PAOLA D, DANZI D, VENDRAMIN G.G, SONNANTE G PLoS ONE; 2015, 10(3):e0120589. doi:10.1371/ journal.pone. 0120589
  • GATTO A., DANZI D., CURCI P.L., KHALDI S., MORGESE A., SONNANTE GI., DE PAOLA D., BLANCO E., SONNANTE G. WILD CARDOON VARIATION AND THE DOMESTICATION OF ARTICHOKE Proceedings of the Joint Meeting AGI-SIBV-SIGA Assisi, Italy - 19/22 September, 2011 ISBN 978-88-904570-2-9
  • DANZI D., IOVENE M., MORGESE A., PIGNONE D., SONNANTE G. CHARACTERIZATION AND UTILIZATION OF A BAC LIBRARY FROM ARTICHOKE Proceedings of the 57th Italian Society of Agricultural Genetics Annual Congress Foggia, Italy - 16/19 September, 2013 ISBN 978-88-904570-3-6
  • JANNI M., DE PAOLA D., CURCI P.L., DANZI D., MORGESE A., SONNANTE G ANALYSIS OF DURUM WHEAT RESPONSE TO NITROGEN STARVATION. Proceedings of the 57th Italian Society of Agricultural Genetics Annual Congress Foggia, Italy - 16/19 September, 2013 ISBN 978-88-904570-3-6
  • CURCI P.L., DE PAOLA D., DANZI D., SONNANTE G. DISCOVERING ARTICHOKE CLOROPLAST GENOME: A POWERFUL RESOURCE FOR PHYLOGENETIC STUDIES AND BARCODING APPLICATIONS. Proceedings of the 57th Italian Society of Agricultural Genetics Annual Congress Foggia, Italy - 16/19 September, 2013 ISBN 978-88-904570-3-6
  • CURCI P. L., DE PAOLA D., DANZI D., SONNANTE G. CYNARA WHOLE CHLOROPLAST GENOME: EVOLUTIONARY IMPLICATIONS AND BARCODING Conference EPSO. Grecia, 1-4 settembre 2013
  • BLANCO E., NEGRO D., SABETTA W., MORGESE A., DANZI D., DE LISI A., SARLI G., SONNANTE G. VALORIZZAZIONE DELLA BIODIVERSITA’ CINARICOLA: CARATTERIZZAZIONE GENETICA E BIOCHIMICA DI ALCUNE VARIETA’ DI CARCIOFO COLTIVATO. X National Conference on Biodiversity. Roma, 3-5 september 2014
  • CURCI P. L.., DE PAOLA D.,DANZI D., SONNANTE G DIVERSITA’ DEL GENOMA CLOROPLASTICO IN CYNARA SVELATA DAL SEQUENZIAMENTO NGS (Next Generation Sequencing) X National Conference on Biodiversity. Roma, 3-5 september 2014
  • ZULUAGA D L., CURCI P. L., DANZI D., SONNANTE G. DIVERSITA’ DELLE RISPOSTE DEI GENI ALLA CARENZA DI AZOTO IN FRUMENTO DURO X National Conference on Biodiversity. Roma, 3-5 september 2014
  • BLANCO E., SABETTA W., DANZI D., MORGESE A., SONNANTE G. INSIGHT INTO THE REGULATION OF FLAVONOID BIOSYNTHESIS IN ARTICHOKE: ISOLATION OF MYB TRASCRIPTION FACTORS Proceedings of the 58th Italian Society of Agricultural Genetics Annual Congress Alghero, Italy - 15/18 September, 2014
  • ZULUAGA D.L., DE PAOLA D., DANZI D., JANNI M., SONNANTE G. NOVEL AND CONSERVED MIRNAS INVOLVED IN PLANT DEVELOPMENT AND NITROGEN METABOLISM IN DURUM WHEAT International Conference: From seed to pasta e beyond. A sustainable durum wheat chain for food security and healthy Lives, Bologna, Italy- 31 May, 2 June, 2015
  • CURCI P.L, DE PAOLA D, DANZI D, SONNANTE G CHLOROPLAST GENOME DIVERSITY IN CYNARA IX International Simposium on Artichoke, Cardoon and their wild relatives. La Plata, Argentina 2015
  • BLANCO E., SABETTA W., DANZI D., MORGESE A., NEGRO D., SARLI G., DE LISI A.,PAOLOCCI F., SONNANTE G REGULATION OF FLAVONOID BIOSYNTHESIS IN ARTICHOKE: ISOLATION AND CHARACTHERIZATION OF A MYB TRANSCRIPTION FACTOR Proceedings of the 59th Italian Society of Agricultural Genetics Annual Congress Milano, Italy -8 /11 September, 2015

Technical support

  • GENETIC MAP OF ARTICHOKE 3 WILD CARDOON: TOWARD A CONSENSUS MAP FOR CYNARA CARDUNCULUS GABRIELLA SONNANTE , ANGELA GATTO ,ANITA MORGESE , FRANCESCO MONTEMURRO ,GIULIO SARLI , EMANUELA BLANCO,DOMENICOPIGNONE Springer-Verlag ; 2011, Volume 123, pp 1215-1229
  • GENETICS AND GENOMICS OF THE GLOBE ARTICHOKE: WHERE HAVE WE GOT TO?- RECENT ADVANCES AT CNR-IGV ITALY GABRIELLA SONNANTE Acta horticulturae International Society for Horticultural Science; 2013, Volume 983, pp 173-177
  • POPULATION STRUCTURE OF CYNARA CARDUNCULUS COMPLEX ANDTHE ORIGIN OF THE CONSPECIFIC CROPS ARTICHOKE AND CARDOON ANGELA GATTO, DOMENICO DE PAOLA, FRANCESCA BAGNOLI, GIOVANNI GIUSEPPE VENDRAMIN, GABRIELLA SONNANTE Annali of Botany ; 2013, Volume 112, pp 855-865
  • THE miRNA OF DURUM WHEAT ISOLATION AND CHARACTERISATION OF CONSERVED AND NOVEL microRNAs AND THEIR TARGET GENES DOMENICO DE PAOLA, DIANA ZULUAGA, GABRIELLA SONNANTE BMC Genomics ; 2016; 17:505

Awards

Award DISBA 2016 for presentation proposal research “Mining of new below-ground traits in a durum wheat germplasm collection” . Grant For 2017 Mobility To Foreign Laboratories

 

Selected Publications
(full list available at CNR People)

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